| 2024 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 28 October – 3 November |
| Edition | 20th |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| Champions | |
| Singles | |
 Federico Agustín Gómez | |
| Doubles | |
 Karol Drzewiecki / Piotr Matuszewski | |
The 2024 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2024 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 28 October and 3 November 2024.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| ARG | Francisco Comesaña | 97 | 1 |
| ARG | Federico Coria | 101 | 2 |
 COL | Daniel Elahi Galán | 113 | 3 |
| ARG | Román Andrés Burruchaga | 129 | 4 |
| ARG | Marco Trungelliti | 135 | 5 |
| ARG | Juan Manuel Cerúndolo | 149 | 6 |
 CZE | Vít Kopřiva | 150 | 7 |
| ARG | Federico Agustín Gómez | 157 | 8 |
The following players received wildcards into the singles main draw:
Francisco Castro Marcos Lee Chan Baratau Ángel VélizThe following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Alex Barrena 
Gerard Campana Lee
Mathys Erhard
Garrett Johns Mariano Kestelboim 
Ryan Nijboer Federico Agustín Gómez def. 
Tomás Barrios Vera 6–1, 6–4. Karol Drzewiecki / Piotr Matuszewski def. 
Luís Britto / Marcelo Zormann 6–4, 7–6(7–2).
