| Rank | Senator (party-state) | Seniority date | Other factors |
|---|
| 1 | Benjamin Ruggles (AJ-OH) | March 4, 1815 | |
| 2 | Mahlon Dickerson (J-NJ) | March 4, 1817 |
| 3 | William Rufus de Vane King (J-AL) | December 14, 1819 |
| 4 | Nehemiah Rice Knight (AJ-RI) | January 9, 1821 |
| 5 | Horatio Seymour (AJ-VT) | March 4, 1821 |
| 6 | Thomas Hart Benton (J-MO) | August 10, 1821 |
| 7 | Samuel Smith (J-MD) | December 17, 1822 |
| 8 | Samuel Bell (AJ-NH) | March 4, 1823 | Former governor |
| 9 | Robert Young Hayne (N-NH) [6] |
| 10 | Josiah Stoddard Johnston (AJ-LA) | January 15, 1824 |
| 11 | Littleton Waller Tazewell (J-VA) [7] | December 7, 1824 |
| 12 | William Hendricks (AJ-IN) | March 4, 1825 | Former representative |
| 13 | Elias Kent Kane (J-IL) | Illinois 24th in population (1820) |
| 14 | Hugh Lawson White (J-TN) | October 28, 1825 |
| 15 | Ashur Robbins (AJ-RI) | October 31, 1825 |
| 16 | Ezekiel Forman Chambers (AJ-VA) | January 24, 1826 |
| 17 | Nathaniel Silsbee (AJ-MA) | May 31, 1826 |
| 18 | Powhatan Ellis (J-MS) [8] | March 4, 1827 | Former senator |
| 19 | John Tyler (J-VA) | Former representative (4 years, 2 months) |
| 20 | Samuel Augustus Foot (AJ-CT) | Former representative (4 years, 0 months) |
| 21 | Isaac Dutton Barnard (J-PA) [9] |
| 22 | Daniel Webster (AJ-MA) | June 8, 1827 |
| 23 | John Holmes (AJ-ME) | January 15, 1829 | Former representative |
| 24 | Charles Edward Dudley (J-NY) |
| 25 | George Mortimer Bibb (J-KY) | March 4, 1829 | Former senator (3 years) |
| 26 | George Michael Troup (J-GA) | Former senator (1 year) |
| 27 | Edward Livingston (J-LA) [10] | Former representative (12 years) |
| 28 | Peleg Sprague (AJ-ME) | Former representative (4 years) |
| 29 | Theodore Frelinghuysen (AJ-NJ) | New Jersey 13th in population (1820) |
| 30 | John Middleton Clayton (AJ-DE) | Delaware 22nd in population (1820) |
| 31 | Felix Grundy (J-TN) | October 19, 1829 |
| 32 | John Forsyth (J-GA) | November 9, 1829 |
| 33 | Bedford Brown (J-NC) | December 9, 1829 |
| 34 | Arnold Naudain (AJ-DE) | January 13, 1830 |
| 35 | George Poindexter (J-MS) | October 15, 1830 |
| 36 | John McCracken Robinson (J-IL) | December 11, 1830 |
| 37 | Gideon Tomlinson (AJ-CT) | March 4, 1831 | Former representative (8 years); Connecticut 14th in population (1820) |
| 38 | Gabriel Moore (J-AL) | Former representative (8 years); Alabama 19th in population (1820) |
| 39 | Willie Person Mangum (J-NC) | Former representative (3 years) |
| 40 | Stephen Decatur Miller (N-NC) [11] | Former representative (2 years) |
| 41 | William Learned Marcy (J-NY) [12] | New York 1st in population (1820) |
| 42 | William Wilkins (J-PA) | Pennsylvania 2nd in population (1820) |
| 43 | Thomas Ewing (AJ-OH) | Ohio 5th in population (1820) |
| 44 | Isaac Hill (J-NH) | New Hampshire 15th in population (1820) |
| 45 | Samuel Prentiss (AJ-VT) | Vermont 16th in population (1820) |
| 46 | Alexander Buckner (J-MO) | Missouri 23rd in population (1820) |
| 47 | Robert Hanna (AJ-IN) [13] | August 19, 1831 |
| 48 | Henry Clay (AJ-KY) | November 10, 1831 |
| George Augustus Waggaman (AJ-LA) | November 15, 1831 |
| George Mifflin Dallas (J-PA) | December 13, 1831 |
| John Tipton (J-IN) | January 3, 1832 |
| John Black (J-MS) | November 12, 1832 |
| William Cabell Rives (J-VA) | December 10, 1832 |
| John Caldwell Calhoun (N-SC) | December 29, 1832 |
|
