10-30cm long flat-bottomed laboratory tube made of glass
Determination of iron by a colorimetric methodDetermination of iron by a colorimetric method, top view
Nessler cylinders (also named color comparison cylinders or color comparing cylinders) are laboratory tubes with a fixed volume, made of glass with optically plane bottom. On the walls there are marks of the nominal stroke volume (usually 100 ml) and possibly one half-way mark (usually 50 ml).
Nessler cylinders with a capacity of 100 cc and 50 cc
Nessler cylinders are used for colorimetric analysis, such as APHA color. The color of the substance contained in a Nessler cylinder is visually compared with the model. The tubes are often used to carry out a series of calibrations of solutions of increasing concentrations, which functions as a comparative scale. To minimize differences in the subjective impression of the color of the solution of the substance to be analyzed, cylinders of a series should have the same characteristics - height, diameter and thickness of glass. Basically there are four types of used cylinders:
high with a capacity of 50 ml (about 300mm in height, approximately 21mm in diameter)
low with a capacity of 50 ml (about 175mm in height, approximately 25mm in diameter)
high with a capacity of 100 ml (about 375mm in height, approximately 24mm in diameter)
low capacity of 100 ml (about 210mm in height, approximately 34mm in diameter)
These parameters (except volume), however, can vary significantly between two cylinders.
Hehnera cylinders differ in that they are scaled to full height.
Due to the spread of automated spectrophotometers, colorimetric assay methods using Nessler cylinders and visual assessment are used very rarely. However, Nessler cylinders are still used as reaction vessels, in the same way as test tubes.
External links
Witold Hermanowicz, Vera Dożańska, John Dojlido, Bohdan Koziorowski: Physico-chemical examination of water and wastewater. New York: Arcade, 1976, pp 19–21. UKD 628.31:628.1.03:543.3.
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