Round of 32
Heat 1
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 1 | Alexandra Engen |  Sweden | Q |
| 2 | 17 | Cécile Ravanel |  France | Q |
| 3 | 16 | Katrin Leumann |  Switzerland | |
| 4 | 32 | Noelia Rodriguez |  Argentina |
Heat 2
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 8 | Anneke Beerten |  Netherlands | Q |
| 2 | 9 | Linda Indergand | Switzerland | Q |
| 3 | 25 | Ana Zupan |  Slovenia | |
| 4 | 24 | Georgia Gould |  United States |
Heat 3
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 4 | Jolanda Neff | Switzerland | Q |
| 2 | 13 | Laura Turpijn | Netherlands | Q |
| 3 | 20 | Kajsa Snihs | Sweden | |
| 4 | 29 | Heidi Rosasen Sandsto |  Norway |
Heat 4
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 5 | Eva Lechner |  Italy | Q |
| 2 | 12 | Anne Terpstra | Netherlands | Q |
| 3 | 28 | Barbara Benkó |  Hungary | |
| 4 | 21 | Mary McConneloug | United States |
Heat 5
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 15 | Elisabeth Brandau |  Germany | Q |
| 2 | 31 | Michelle Hediger | Switzerland | Q |
| 3 | 18 | Maaris Meier |  Estonia | |
| 4 | 2 | Jenny Rissveds | Sweden | DNF |
Heat 6
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 10 | Anna Oberparleiter | Italy | Q |
| 2 | 7 | Aleksandra Dawidowicz |  Poland | Q |
| 3 | 26 | Rosara Joseph |  New Zealand | |
| 4 | 23 | Alessia Bulleri | Italy |
Heat 7
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 3 | Lea Davison | United States | Q |
| 2 | 19 | Serena Calvetii | Italy | Q |
| 3 | 30 | Rowena Fry (cyclist) |  Australia | |
| 4 | 14 | Andrea Waldis | Switzerland |
Heat 8
| Rank | Seed | Athlete | Country | Note |
|---|---|---|---|---|
| 1 | 6 | Ramona Forchini | Switzerland | Q |
| 2 | 11 | Kathrina Stirnemann | Switzerland | Q |
| 3 | 22 | Ingrid Sofie Jacobsen | Norway | |
| 4 | 27 | Pavla Havliková |  Czech Republic |
