Arp2/3 complex

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Atomic structure of bovine Arp2/3 complex (PDB code: 1k8k). Color coding for subunits: Arp3, orange; Arp2, marine (subunits 1 & 2 not resolved and thus not shown); p40, green; p34, ice blue; p20, dark blue; p21, magenta; p16, yellow. Arp2 3 complex.png
Atomic structure of bovine Arp2/3 complex (PDB code: 1k8k). Color coding for subunits: Arp3, orange; Arp2, marine (subunits 1 & 2 not resolved and thus not shown); p40, green; p34, ice blue; p20, dark blue; p21, magenta; p16, yellow.

Arp2/3 complex (Actin Related Protein 2/3 complex) is a seven-subunit protein complex that plays a major role in the regulation of the actin cytoskeleton. It is a major component of the actin cytoskeleton and is found in most eukaryotic cells. [2] Two of its subunits, the Actin-Related Proteins ARP2 and ARP3, closely resemble the structure of monomeric actin and serve as nucleation sites for new actin filaments. The complex binds to the sides of existing ("mother") filaments and initiates growth of a new ("daughter") filament at a distinctive 70-degree angle from the mother. Branched actin networks are created as a result of this nucleation of new filaments. The regulation of rearrangements of the actin cytoskeleton is important for processes like cell locomotion, phagocytosis, and the intracellular motility of lipid vesicles.

Contents

The Arp2/3 complex was identified in 1994 (preliminarily described as "unconventional actins") by affinity chromatography from the human pathogen Acanthamoeba castellanii, [3] though it had been previously isolated in 1989 in a search for proteins that bind to actin filaments in Drosophila melanogaster embryos. [4]

Mechanisms of actin polymerization by Arp2/3

Side branching model of the Arp2/3 complex. Activated Arp2/3 complex binds to the side of a "mother" actin filament. Both Arp2 and Arp3 form the first two subunits in the new "daughter" filament. Arp23 side branching model.png
Side branching model of the Arp2/3 complex. Activated Arp2/3 complex binds to the side of a "mother" actin filament. Both Arp2 and Arp3 form the first two subunits in the new "daughter" filament.
Barbed end branching model of the Arp2/3 complex. Activated Arp2/3 competes with capping proteins to bind to the barbed end of an actin filament. Arp2 remains bound to the mother filament, while Arp3 is outside. The two Arp subunits form the first subunits of each branch and the two branches continue to grow by addition of G-actin to each Arp Arp23 barbed end branching model.png
Barbed end branching model of the Arp2/3 complex. Activated Arp2/3 competes with capping proteins to bind to the barbed end of an actin filament. Arp2 remains bound to the mother filament, while Arp3 is outside. The two Arp subunits form the first subunits of each branch and the two branches continue to grow by addition of G-actin to each Arp

Many actin-related molecules create a free barbed end for polymerization by uncapping or severing pre-existing filaments and using these as actin nucleation cores. However, the Arp2/3 complex stimulates actin polymerization by creating a new nucleation core. Actin nucleation is an initial step in the formation of an actin filament. The nucleation core activity of Arp2/3 is activated by Nucleation Promoting Factors (NPFs) including members of the Wiskott-Aldrich syndrome family protein (WASP, N-WASP, WAVE, and WASH proteins). The V domain of a WASP protein interacts with actin monomers while the CA region associates with the Arp2/3 complex to create a nucleation core. However, de novo nucleation followed by polymerization is not sufficient to form integrated actin networks, since these newly synthesized polymers would not be associated with pre-existing filaments. Thus, the Arp2/3 complex binds to pre-existing filaments so that the new filaments can grow on the old ones and form a functional actin cytoskeleton. [5] Capping proteins limit actin polymerization to the region activated by the Arp2/3 complex, and the elongated filament ends are recapped to prevent depolymerization and thus conserve the actin filament. [6]

The Arp2/3 complex simultaneously controls nucleation of actin polymerization and branching of filaments. Moreover, autocatalysis is observed during Arp2/3-mediated actin polymerization. In this process, the newly formed filaments activate other Arp2/3 complexes, facilitating the formation of branched filaments.

The mechanism of actin filament initiation by Arp2/3 has been disputed. The question is where the complex binds the filament and nucleates a "daughter" filament. Historically two models have been proposed. Recent results favour the side branching model, in which the Arp2/3 complex binds to the side of a pre-existing ("mother") filament at a point different from the nucleation site. Although the field lacks a high-resolution crystal structure, data from electron microscopy, [7] [8] [9] together with biochemical data on the filament nucleation and capping mechanisms of the Arp2/3 complex, [10] favour side branching. In the alternative barbed end branching model, Arp2/3 only associates at the barbed end of growing filaments, allowing for the elongation of the original filament and the formation of a branched filament., [11] a model based on kinetic analysis and optical microscopy.

Recent computer docking, independently confirmed by EM data, favors a side-branching model. ARPC2 and ARPC4 together form an area that attach the base of the branch to the side of a mother filament. [12] Large conformational changes occur on nucleotide and WASP binding. [9] [13]

Cellular uses of Arp2/3

The Arp2/3 complex appears to be important in a variety of specialized cell functions that involve the actin cytoskeleton. The complex is found in cellular regions characterized by dynamic actin filament activity: in macropinocytic cups, in the leading edge of motile cells (lamellipodia), and in motile actin patches in yeast. [14] In mammals and the social amoeba Dictyostelium discoideum [15] [16] it is required for phagocytosis. The complex has also been shown to be involved in the establishment of cell polarity and the migration of fibroblast monolayers in a wound-healing model. [17] In mammalian oocytes, the Arp2/3 complex is involved in oocyte asymmetric division and polar body emission, which result from the failure of spindle migration (a unique feature of oocyte division) and cytokinesis. [18] Moreover, enteropathogenic organisms like Listeria monocytogenes and Shigella use the Arp2/3 complex for actin-polymerization- dependent rocketing movements. [19] The Arp2/3 complex also regulates the intracellular motility of endosomes, lysosomes, pinocytic vesicles, and mitochondria. [20] Moreover, recent studies show that the Arp2/3 complex is essential for proper polar cell expansion in plants. Arp2/3 mutations in Arabidopsis thaliana result in abnormal filament organization, which in turn affects the expansion of trichomes, pavement cells, hypocotyl cells, and root hair cells. [21] [22] Chemical inhibition or genetic mutation of the Chlamydomonas reinhardtii Arp2/3 complex decreases the length of flagella. [23] [24]

Subunits

Arp2,3komplex with subunits.jpg

The Arp2/3 complex is composed of seven subunits: Arp2/ACTR2, Arp3/ACTR3, p41/ARPC1A&B/Arc40/Sop2/p40, p34/ARPC2/ARC35/p35, p21/ARPC3/ARC18/p19, p20/ARPC4/ARC19/p18, p16/ARPC5/ARC15/p14. [25] [26] The subunits Arp2 and Arp3 closely resemble monomeric actin allowing for a thermodynamically stable actin-like dimer. p41 has been proposed to interact with nucleation promoting factors (NPFs) because it is only known to have minor contacts with the mother filament and there is a major loss of nucleation efficiency in the absence of p41. p34 and p20 dimerize to form a structural backbone that mediates the interaction with the mother filament. p21 forms a bridge between Arp3 and the mother filament, increasing nucleation efficiency. p16 tethers Arp2 to the rest of the complex. [27]

Some subunits of the Arp2/3 complex exist in multiple isoforms with distinct activities. For example, the ARPC1 subunit has two isoforms in mammals: ARPC1A and ARPC1B. The latter appears particularly important for maturation of platelets. [28] Similarly, ARPC5 has a homolog ARPC5L (ARPC5-like). Compared to ARPC5, ARPC5L supports more rapid and dynamic reorganization of filaments in regions such as lamellipodia involved in cell motility. [29]

Structural insights

The Arp2/3 complex is composed of seven subunits: two actin-related proteins (Arp2 and Arp3) and five additional subunits (ARPC1–ARPC5). In its inactive state, the complex adopts a compact conformation where Arp2 and Arp3 are spatially separated and not aligned in a way that mimics an actin dimer. This conformation prevents spontaneous actin nucleation. [30]

Upon activation by nucleation-promoting factors (NPFs)—such as WASP—the Arp2/3 complex undergoes a dramatic conformational rearrangement. Cryo-electron microscopy and molecular dynamics simulations have revealed that the binding of NPFs and ATP induces repositioning of Arp2 and Arp3 into a short-pitch actin-like dimer, the geometry required for nucleating a new actin filament branch. [31] This transition forms a structural "template" that resembles a barbed-end actin nucleus, which then elongates by recruiting actin monomers.

The core subunits, particularly ARPC2 and ARPC4, form a structural scaffold that binds the side of an existing ("mother") actin filament at a 70-degree angle. This binding provides the platform from which a new ("daughter") filament can branch. [32] Structural data show that ATP binding is essential for this transition. [32]

Cellular and physiological roles

The Arp2/3 complex is essential for the spatial and temporal regulation of actin cytoskeleton dynamics. One of the most well-characterized roles of the Arp2/3 complex is in cell motility, particularly through the formation of lamellipodia. This is vital in processes such as cell movement and vesicle trafficking. The complex, activated by nucleation-promoting factors like WASP and WAVE, catalyzes the branching of actin filaments to push the plasma membrane forward. [33]

In neuronal development, the Arp2/3 complex plays a pivotal role in axon guidance, organization of radial glial cells, and dendritic spine formation. Disruption of Arp2/3 function in mouse models leads to severe abnormalities in the cortical layering of neurons, loss of neuronal polarity, and impaired synaptic plasticity, indicating that actin nucleation is critical for establishing and maintaining neuronal networks. [34] [35]

In the immune system, Arp2/3-mediated actin remodeling is necessary for phagocytosis, immune synapse formation, and cell migration. [28]

References

  1. ↑ Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S, Pollard TD (November 2001). "Crystal structure of Arp2/3 complex". Science. 294 (5547): 1679–84. Bibcode:2001Sci...294.1679R. doi:10.1126/science.1066333. PMID   11721045. S2CID   18088124.
  2. ↑ Veltman DM, Insall RH (August 2010). "WASP family proteins: their evolution and its physiological implications". Molecular Biology of the Cell. 21 (16): 2880–93. doi:10.1091/mbc.E10-04-0372. PMC   2921111 . PMID   20573979.
  3. ↑ Machesky LM, Atkinson SJ, Ampe C, Vandekerckhove J, Pollard TD (October 1994). "Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose". The Journal of Cell Biology. 127 (1): 107–15. doi:10.1083/jcb.127.1.107. PMC   2120189 . PMID   7929556.
  4. ↑ Miller KG, Field CM, Alberts BM (December 1989). "Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography". The Journal of Cell Biology. 109 (6 Pt 1): 2963–75. doi:10.1083/jcb.109.6.2963. PMC   2115944 . PMID   2512303.
  5. ↑ Pollard TD (2007). "Regulation of actin filament assembly by Arp2/3 complex and formins". Annual Review of Biophysics and Biomolecular Structure. 36: 451–77. doi:10.1146/annurev.biophys.35.040405.101936. PMID   17477841.
  6. ↑ Aguda AH, Burtnick LD, Robinson RC (March 2005). "The state of the filament". EMBO Reports. 6 (3): 220–6. doi:10.1038/sj.embor.7400363. PMC   1299273 . PMID   15741975.
  7. ↑ Egile C, Rouiller I, Xu XP, Volkmann N, Li R, Hanein D (November 2005). "Mechanism of filament nucleation and branch stability revealed by the structure of the Arp2/3 complex at actin branch junctions". PLOS Biology. 3 (11) e383. doi: 10.1371/journal.pbio.0030383 . PMC   1278936 . PMID   16262445.
  8. ↑ Volkmann N, Amann KJ, Stoilova-McPhie S, Egile C, Winter DC, Hazelwood L, Heuser JE, Li R, Pollard TD, Hanein D (September 2001). "Structure of Arp2/3 complex in its activated state and in actin filament branch junctions". Science. 293 (5539): 2456–9. Bibcode:2001Sci...293.2456V. doi: 10.1126/science.1063025 . PMID   11533442. S2CID   17427295.
  9. 1 2 Rouiller I, Xu XP, Amann KJ, Egile C, Nickell S, Nicastro D, Li R, Pollard TD, Volkmann N, Hanein D (March 2008). "The structural basis of actin filament branching by the Arp2/3 complex". The Journal of Cell Biology. 180 (5): 887–95. doi:10.1083/jcb.200709092. PMC   2265399 . PMID   18316411.
  10. ↑ Dayel MJ, Mullins RD (April 2004). "Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2". PLOS Biology. 2 (4) E91. doi: 10.1371/journal.pbio.0020091 . PMC   387265 . PMID   15094799.
  11. ↑ Pantaloni D, Boujemaa R, Didry D, Gounon P, Carlier MF (July 2000). "The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins". Nature Cell Biology. 2 (7): 385–91. doi:10.1038/35017011. PMID   10878802. S2CID   28209883.
  12. ↑ Goley, ED; Rammohan, A; Znameroski, EA; Firat-Karalar, EN; Sept, D; Welch, MD (4 May 2010). "An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability". Proceedings of the National Academy of Sciences of the United States of America. 107 (18): 8159–64. Bibcode:2010PNAS..107.8159G. doi: 10.1073/pnas.0911668107 . PMC   2889539 . PMID   20404198.
  13. ↑ Padrick, S. B.; Doolittle, L. K.; Brautigam, C. A.; King, D. S.; Rosen, M. K. (16 August 2011). "Arp2/3 complex is bound and activated by two WASP proteins". Proceedings of the National Academy of Sciences. 108 (33): E472–E479. doi: 10.1073/pnas.1100236108 . ISSN   0027-8424. PMC   3158169 . PMID   21676863.
  14. ↑ Warren DT, Andrews PD, Gourlay CW, Ayscough KR (April 2002). "Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics" . Journal of Cell Science. 115 (Pt 8): 1703–15. doi: 10.1242/jcs.115.8.1703 . PMID   11950888.
  15. ↑ May RC, Caron E, Hall A, Machesky LM (April 2000). "Involvement of the Arp2/3 complex in phagocytosis mediated by FcgammaR or CR3". Nature Cell Biology. 2 (4): 246–8. doi:10.1038/35008673. PMID   10783245. S2CID   33742268.
  16. ↑ Insall R, Müller-Taubenberger A, Machesky L, Köhler J, Simmeth E, Atkinson SJ, Weber I, Gerisch G (November 2001). "Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis". Cell Motility and the Cytoskeleton. 50 (3): 115–28. doi:10.1002/cm.10005. PMID   11807934.
  17. ↑ Magdalena J, Millard TH, Etienne-Manneville S, Launay S, Warwick HK, Machesky LM (February 2003). "Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models". Molecular Biology of the Cell. 14 (2): 670–84. doi:10.1091/mbc.E02-06-0345. PMC   150000 . PMID   12589062.
  18. ↑ Yi K, Unruh JR, Deng M, Slaughter BD, Rubinstein B, Li R (August 2011). "Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes". Nature Cell Biology. 13 (10): 1252–8. doi:10.1038/ncb2320. PMC   3523671 . PMID   21874009.
  19. ↑ Cossart P (June 2000). "Actin-based motility of pathogens: the Arp2/3 complex is a central player". Cellular Microbiology. 2 (3): 195–205. doi: 10.1046/j.1462-5822.2000.00053.x . PMID   11207576. S2CID   44343534.
  20. ↑ Mathur J (April 2005). "The ARP2/3 complex: giving plant cells a leading edge". BioEssays. 27 (4): 377–87. Bibcode:2005BiEss..27..377M. doi:10.1002/bies.20206. PMID   15770684. S2CID   423293.
  21. ↑ Bannigan A, Baskin TI (December 2005). "Directional cell expansion--turning toward actin". Current Opinion in Plant Biology. 8 (6): 619–24. Bibcode:2005COPB....8..619B. doi:10.1016/j.pbi.2005.09.002. OSTI   2483287. PMID   16181803.
  22. ↑ Xu J, Scheres B (December 2005). "Cell polarity: ROPing the ends together". Current Opinion in Plant Biology. 8 (6): 613–8. Bibcode:2005COPB....8..613X. doi:10.1016/j.pbi.2005.09.003. hdl: 1874/21120 . PMID   16182602. S2CID   23098801.
  23. ↑ Avasthi, Prachee; Onishi, Masayuki; Karpiak, Joel; Yamamoto, Ryosuke; Mackinder, Luke; Jonikas, Martin C.; Sale, Winfield S.; Shoichet, Brian; Pringle, John R.; Marshall, Wallace F. (September 2014). "Actin Is Required for IFT Regulation in Chlamydomonas reinhardtii". Current Biology. 24 (17): 2025–2032. Bibcode:2014CBio...24.2025A. doi:10.1016/j.cub.2014.07.038. ISSN   0960-9822. PMC   4160380 . PMID   25155506.
  24. ↑ Bigge, Brae M.; Rosenthal, Nicholas E.; Sept, David; Schroeder, Courtney M.; Avasthi, Prachee (2023). "Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex–dependent endocytosis". Molecular Biology of the Cell. 34 (4) ar24. bioRxiv   10.1101/2020.11.24.396002 . doi:10.1091/mbc.E22-09-0443.
  25. ↑ Goley ED, Welch MD (October 2006). "The ARP2/3 complex: an actin nucleator comes of age". Nature Reviews Molecular Cell Biology. 7 (10): 713–26. Bibcode:2006NRMCB...7..713G. doi: 10.1038/nrm2026 . PMID   16990851. S2CID   20645116.
  26. ↑ Pizarro-Cerdá, Javier; Chorev, Dror Shlomo; Geiger, Benjamin; Cossart, Pascale (February 2017). "The Diverse Family of Arp2/3 Complexes". Trends in Cell Biology. 27 (2): 93–100. doi:10.1016/j.tcb.2016.08.001. ISSN   0962-8924. PMC   7098815 . PMID   27595492.
  27. ↑ Pizarro-Cerdá J, Chorev DS, Geiger B, Cossart P (February 2017). "The Diverse Family of Arp2/3 Complexes" (PDF). Trends in Cell Biology. 27 (2): 93–100. doi:10.1016/j.tcb.2016.08.001. PMC   7098815 . PMID   27595492.
  28. 1 2 Kahr, Walter H. A.; Pluthero, Fred G.; Elkadri, Abdul; Warner, Neil; Drobac, Marko; Chen, Chang Hua; Lo, Richard W.; Li, Ling; Li, Ren; Li, Qi; Thoeni, Cornelia; Pan, Jie; Leung, Gabriella; Lara-Corrales, Irene; Murchie, Ryan (2017-04-03). "Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease". Nature Communications. 8 (1) 14816. Bibcode:2017NatCo...814816K. doi:10.1038/ncomms14816. ISSN   2041-1723. PMC   5382316 . PMID   28368018.
  29. ↑ Abella, Jasmine V. G.; Galloni, Chiara; Pernier, Julien; Barry, David J.; Kjær, Svend; Carlier, Marie-France; Way, Michael (January 2016). "Isoform diversity in the Arp2/3 complex determines actin filament dynamics" . Nature Cell Biology. 18 (1): 76–86. doi:10.1038/ncb3286. ISSN   1476-4679. PMID   26655834.
  30. ↑ Robinson, Robert C.; Turbedsky, Kirsi; Kaiser, Donald A.; Marchand, Jean-Baptiste; Higgs, Henry N.; Choe, Senyon; Pollard, Thomas D. (2001-11-23). "Crystal Structure of Arp2/3 Complex" . Science. 294 (5547): 1679–1684. Bibcode:2001Sci...294.1679R. doi:10.1126/science.1066333. PMID   11721045.
  31. ↑ Shaaban, Mohammed; Chowdhury, Saikat; Nolen, Brad J. (November 2020). "Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state". Nature Structural & Molecular Biology. 27 (11): 1009–1016. doi:10.1038/s41594-020-0481-x. ISSN   1545-9985. PMC   8717925 . PMID   32839613.
  32. 1 2 Rouiller, Isabelle; Xu, Xiao-Ping; Amann, Kurt J.; Egile, Coumaran; Nickell, Stephan; Nicastro, Daniela; Li, Rong; Pollard, Thomas D.; Volkmann, Niels; Hanein, Dorit (2008-03-03). "The structural basis of actin filament branching by the Arp2/3 complex". Journal of Cell Biology. 180 (5): 887–895. doi:10.1083/jcb.200709092. ISSN   0021-9525. PMC   2265399 . PMID   18316411.
  33. ↑ Takenawa, Tadaomi; Suetsugu, Shiro (January 2007). "The WASP–WAVE protein network: connecting the membrane to the cytoskeleton" . Nature Reviews Molecular Cell Biology. 8 (1): 37–48. Bibcode:2007NRMCB...8...37T. doi:10.1038/nrm2069. ISSN   1471-0080. PMID   17183359.
  34. ↑ Wang, Pei-Shan; Chou, Fu-Sheng; Ramachandran, Sreekumar; Xia, Sheng; Chen, Huei-Ying; Guo, Fengli; Suraneni, Praveen; Maher, Brady J.; Li, Rong (2016-08-01). "Crucial roles of the Arp2/3 complex during mammalian corticogenesis". Development. 143 (15): 2741–2752. doi:10.1242/dev.130542. ISSN   0950-1991. PMC   5004905 . PMID   27385014.
  35. ↑ Kim, Il Hwan; Racz, Bence; Wang, Hong; Burianek, Lauren; Weinberg, Richard; Yasuda, Ryohei; Wetsel, William C.; Soderling, Scott H. (2013-04-03). "Disruption of Arp2/3 Results in Asymmetric Structural Plasticity of Dendritic Spines and Progressive Synaptic and Behavioral Abnormalities". J. Neurosci. 33 (14): 6081–6092. doi:10.1523/JNEUROSCI.0035-13.2013. ISSN   1529-2401. PMC   3656411 . PMID   23554489.

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