Colombina is a stock character and Harlequin's mistress.
Colombina or Columbina may also refer to:
- Colombina Parra (born 1970), Chilean musician
- Colombina, a fictional character from Pinocchio (2019 film)
- Institución Colombina (Colombina Institute), Seville, Spain; an administrative unit of several libraries and archives
- La Colombina, a library administered by the institute
- La Colombina, a band founded by Josep Cabré
- Colombina, the dove-shaped rocket traditionally fired in Florence, Italy; see Traditions of Italy
- Dulces Colombina, a multinational candy brand
- Colombina, a Dutch theatre award
- Columbina, a character in Genshin Impact
