DAOY (biology)

Last updated

DAOY is a widely used human medulloblastoma cell line. It has epithelial morphology and was obtained from a 4-year-old Caucasian boy in 1985. [1] It can be obtained from the American Type Culture Collection (ATCC) where it is known by the catalog number HTB-186. [2] Its proteome and phenotype have been investigated in many publications. [3] [4] [5]

DAOY is most commonly grown in Eagle's minimal essential medium with 10% FBS and antibiotics. It is incubated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.

Related Research Articles

In cell biology, U87 is a human glioblastoma cell line that is commonly used in brain cancer research.

GPR143 Protein-coding gene in the species Homo sapiens

G-protein coupled receptor 143 is a protein encoded by the GPR143 gene in humans.

PTCH1 Protein-coding gene in the species Homo sapiens

Protein patched homolog 1 is a protein that is the member of the patched family and in humans is encoded by the PTCH1 gene.

DDX3X Protein-coding gene in the species Homo sapiens

ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene.

HIC1 Protein-coding gene in the species Homo sapiens

Hypermethylated in cancer 1 protein is a protein that in humans is encoded by the HIC1 gene.

KLC1 Gene of the kinesin light chain family

Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene.

GDI2

Rab GDP dissociation inhibitor beta is a protein that in humans is encoded by the GDI2 gene.

RARRES3

Retinoic acid receptor responder protein 3 is a protein that in humans is encoded by the RARRES3 gene.

TMSB10

Thymosin beta-10 is a protein that in humans is encoded by the TMSB10 gene. TMSB10 is a member of the beta-thymosin family of peptides.

SUFU Protein-coding gene in the species Homo sapiens

Suppressor of fused homolog is a protein that in humans is encoded by the SUFU gene. In molecular biology, the protein domain suppressor of fused protein (Sufu) has an important role in the cell. The Sufu is important in negatively regulating an important signalling pathway in the cell, the Hedgehog signalling pathway (HH). This particular pathway is crucial in embryonic development. There are several homologues of Sufu, found in a wide variety of organisms.

DNAJB11

DnaJ homolog subfamily B member 11 is a protein that in humans is encoded by the DNAJB11 gene.

TPM4 Protein-coding gene in the species Homo sapiens

Tropomyosin alpha-4 chain is a protein that in humans is encoded by the TPM4 gene.

DNAJA2

DnaJ homolog subfamily A member 2 is a protein that in humans is encoded by the DNAJA2 gene.

PHF3 Protein-coding gene in the species Homo sapiens

PHD finger protein 3 is a protein that in humans is encoded by the PHF3 gene.

Gigaxonin

Gigaxonin also known as kelch-like protein 16 is a protein that in humans is encoded by the GAN gene.

STK10

Serine/threonine-protein kinase 10 is an enzyme that in humans is encoded by the STK10 gene.

NUDT6

Nucleoside diphosphate-linked moiety X motif 6 is a protein that in humans is encoded by the NUDT6 gene.

MYO18B

Myosin-XVIIIb is a protein that in humans is encoded by the MYO18B gene.

Capicua (protein) Protein-coding gene in the species Homo sapiens

Capicua transcriptional repressor is a protein that in humans is encoded by the CIC gene. Capicua functions as a transcriptional repressor in a way that ensures its impact on the progression of cancer, and plays a significant role in the operation of the central nervous system through its interaction with ataxin 1. The name of the protein derives from the Catalan expression cap-i-cua which literally translates to "head-and-tail".

Rootletin

Rootletin also known as ciliary rootlet coiled-coil protein (CROCC) is a protein that in humans is encoded by the CROCC gene. Rootletin is a component of the ciliary rootlet, and, together with CEP68 and CEP250, is required for centrosome cohesion.

References

  1. Jacobsen PF, Jenkyn DJ, Papadimitriou JM (Sep 1985). "Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice". J Neuropathol Exp Neurol. 44 (5): 472–485. doi:10.1097/00005072-198509000-00003. PMID   2993532.
  2. "HTB-186". ATCC.
  3. Martelli C, et al. (Sep 2016). "Top-down proteomic characterization of DAOY medulloblastoma tumor cell line". EuPA Open Proteomics. 12: 13–21. doi:10.1016/j.euprot.2016.03.015. PMC   5988510 . PMID   29900115.
  4. Srivastava VK, Nalbantoglu J (Oct 2008). "Flow cytometric characterization of the DAOY medulloblastoma cell line for the cancer stem-like phenotype". Cytometry A. 73 (10): 940–948. doi: 10.1002/cyto.a.20633 . PMID   18773455.