DAOY is a widely used human medulloblastoma cell line. It has epithelial morphology and was obtained from a 4-year-old Caucasian boy in 1985. [1] It can be obtained from the American Type Culture Collection (ATCC) where it is known by the catalog number HTB-186. [2] Its proteome and phenotype have been investigated in many publications. [3] [4] [5]
DAOY is most commonly grown in Eagle's minimal essential medium with 10% FBS and antibiotics. It is incubated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.
In cell biology, U87 is a human glioblastoma cell line that is commonly used in brain cancer research.
G-protein coupled receptor 143 is a protein encoded by the GPR143 gene in humans.
Protein patched homolog 1 is a protein that is the member of the patched family and in humans is encoded by the PTCH1 gene.
ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene.
Hypermethylated in cancer 1 protein is a protein that in humans is encoded by the HIC1 gene.
Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene.
Rab GDP dissociation inhibitor beta is a protein that in humans is encoded by the GDI2 gene.
Retinoic acid receptor responder protein 3 is a protein that in humans is encoded by the RARRES3 gene.
Thymosin beta-10 is a protein that in humans is encoded by the TMSB10 gene. TMSB10 is a member of the beta-thymosin family of peptides.
Suppressor of fused homolog is a protein that in humans is encoded by the SUFU gene. In molecular biology, the protein domain suppressor of fused protein (Sufu) has an important role in the cell. The Sufu is important in negatively regulating an important signalling pathway in the cell, the Hedgehog signalling pathway (HH). This particular pathway is crucial in embryonic development. There are several homologues of Sufu, found in a wide variety of organisms.
DnaJ homolog subfamily B member 11 is a protein that in humans is encoded by the DNAJB11 gene.
Tropomyosin alpha-4 chain is a protein that in humans is encoded by the TPM4 gene.
DnaJ homolog subfamily A member 2 is a protein that in humans is encoded by the DNAJA2 gene.
PHD finger protein 3 is a protein that in humans is encoded by the PHF3 gene.
Gigaxonin also known as kelch-like protein 16 is a protein that in humans is encoded by the GAN gene.
Serine/threonine-protein kinase 10 is an enzyme that in humans is encoded by the STK10 gene.
Nucleoside diphosphate-linked moiety X motif 6 is a protein that in humans is encoded by the NUDT6 gene.
Myosin-XVIIIb is a protein that in humans is encoded by the MYO18B gene.
Capicua transcriptional repressor is a protein that in humans is encoded by the CIC gene. Capicua functions as a transcriptional repressor in a way that ensures its impact on the progression of cancer, and plays a significant role in the operation of the central nervous system through its interaction with ataxin 1. The name of the protein derives from the Catalan expression cap-i-cua which literally translates to "head-and-tail".
Rootletin also known as ciliary rootlet coiled-coil protein (CROCC) is a protein that in humans is encoded by the CROCC gene. Rootletin is a component of the ciliary rootlet, and, together with CEP68 and CEP250, is required for centrosome cohesion.