The following is a list of mayors of Seguin, Texas.
| Years of service | Mayor | Image |
|---|---|---|
| 1838–1846 | Established - Unincorporated - Part of Gonzales County [1] - Asa J. L. Sowell [2] | |
| 1846–53 | Michael H. Erskine - Chief Justice - 1st City Charter - Part of Guadalupe County [3] | |
| 1853 | John R. King - 1st Mayor - Incorporated [4] | |
| 1853–57 | John D. Anderson [5] - Elected - Incorporated | |
| 1858 | John Ireland [6] - 18th Governor of Texas |  |
| 1858–59 | Joseph F. Johnson [7] [8] | |
| 1859–61 | Washington E. Goodrich [9] [10] | |
| 1862–65 | The City Charter was not maintained during the Civil War. | |
| 1866–72 | March 30, 1870 - The United States Congress readmits Texas into the Union. | |
| 1873–74 | William M. Rust [11] | |
| 1874–78 | R. J. Burges | |
| 1878–80 | T. D. Johnson | |
| 1882–87 | John A. Neill | |
| 1887–89 | F. H. Vaughn | |
| 1890–1910 | Joseph Zorn. Jr. [12] | |
| 1910–12 | Hilmar H. Weinert [13] | |
| 1912–22 | Charles Bruns | |
| 1922–26 | Robert E. Blumberg | |
| 1926–28 | A. P. Stautzenberger | |
| 1928–38 | Maximilian H. "Max" Starcke [14] | |
| 1938–60 | Roger W. Moore | |
| 1960–62 | Winfred Owen | |
| 1962–66 | Richard Joseph "Joe" Burges | |
| 1966–82 | Alfred H. Koebig |  |
| 1983–89 | Betty Jean Jones |  |
| 1990–95 | Ed Gotthardt | |
| 1996–2005 | Mark Stautzenberger | |
| 2006–12 | Betty Ann Matthies | |
| 2013–2019 | Don Keil [15] | |
| 2020–Present | Donna Dodgen [16] |