Serine/arginine-rich splicing factor 1

Last updated
SRSF1
Protein SFRS1 PDB 1x4a.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases SRSF1 , ASF, SF2, SF2p33, SFRS1, SRp30a, ASF/SF2, serine/arginine-rich splicing factor 1, serine and arginine rich splicing factor 1
External IDs OMIM: 600812 MGI: 98283 HomoloGene: 31411 GeneCards: SRSF1
Orthologs
SpeciesHumanMouse
Entrez

6426

110809

Ensembl

ENSG00000136450

ENSMUSG00000018379

UniProt

Q07955

Q6PDM2

RefSeq (mRNA)

NM_001078166
NM_006924

NM_001078167
NM_173374

RefSeq (protein)

NP_001071634
NP_008855

NP_001071635
NP_775550

Location (UCSC) Chr 17: 58 – 58.01 Mb Chr 11: 87.94 – 87.94 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Serine/arginine-rich splicing factor 1 (SRSF1) also known as alternative splicing factor 1 (ASF1), pre-mRNA-splicing factor SF2 (SF2) or ASF1/SF2 is a protein that in humans is encoded by the SRSF1 gene. [5] ASF/SF2 is an essential sequence specific splicing factor involved in pre-mRNA splicing. [6] [7] [8] SRSF1 is the gene that codes for ASF/SF2 [9] and is found on chromosome 17. The resulting splicing factor is a protein of approximately 33 kDa. [10] ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing. [7] In addition to being involved in the splicing process, ASF/SF2 also mediates post-splicing activities, such as mRNA nuclear export and translation. [11]

Contents

Structure

ASF/SF2 is an SR protein, and as such, contains two functional modules: an arginine-serine rich region (RS domain), where the bulk of ASF/SF2 regulation takes place, and two RNA recognition motifs (RRMs), through which ASF/SF2 interacts with RNA and other splicing factors. [12] [13] These modules have different functions within general splicing factor function. [13]

Splicing

ASF/SF2 is an integral part of numerous components of the splicing process. ASF/SF2 is required for 5’ splice site cleavage and selection, and is capable of discriminating between cryptic and authentic splice sites. [10] Subsequent lariat formation during the first chemical step of pre-mRNA splicing also requires ASF/SF2. [10] ASF/SF2 promotes recruitment of the U1 snRNP to the 5’ splice site, and bridges the 5’ and 3’ splice sites to facilitate splicing reactions. [8] ASF/SF2 also associates with the U2 snRNP. [15] During the reaction, ASF/SF2 promotes the use of intron proximal sites and hinders the use of intron distal sites, affecting alternative splicing. [16] [17] Alternative splicing is affected by ASF/SF2 in a concentration-dependent manner; differing concentrations of ASF/SF2 is a mechanism for alternative splicing regulation, and will result in differing amounts of product isoforms. [6] ASF/SF2 accomplishes this regulation through direct or indirect binding to exonic splicing enhancer (ESE) sequences. [16]

Post-splicing

ASF/SF2, in the presence of elF4E, promotes the initiation of translation of ribosome-bound mRNA by suppressing the activity of 4E-BP and recruiting molecules for further regulation of translation. [11] ASF/SF2 interacts with the nuclear export protein TAP in a regulated manner, controlling the export of mature mRNA from the nucleus. [18] An increase in cellular ASF/SF2 also will increase the efficiency of nonsense-mediated mRNA decay (NMD), favoring NMD that occurs before mRNA release from the nucleus over NMD that occurs after mRNA export from the nucleus to the cytoplasm. [19] This shift in NMD caused by increased ASF/SF2 is accompanied by overall enhancement of the pioneer round of translation, through elF4E-bound mRNA translation and subsequent translationally active ribosomes, increased association of pioneer translation initiation complexes with ASF/SF2, and increased levels of active TAP. [19]

Regulation through phosphorylation

ASF/SF2 has the ability to be phosphorylated at the serines in its RS domain by the SR specific protein kinase, SRPK1. [13] SRPK1 and ASF/SF2 form an unusually stable complex of apparent Kd of 50nM. [12] [18] SRPK1 selectively phosphorylates up to twelve serines in the RS domain of ASF/SF2 through a directional and processive mechanism, moving from the C terminus to the N terminus. [13] This multi-phosphorylation directs ASF/SF2 to the nucleus, influencing a number of protein-protein interactions associated with splicing. [13] ASF/SF2's function in export of mature mRNA from the nucleus is dependent on its phosphorylation state; dephosphorylation of ASF/SF2 facilitates binding to TAP, [13] while phosphorylation directs ASF/SF2 to nuclear speckles. [18] Both phosphorylation and dephosphorylation of ASF/SF2 are important and necessary for proper splicing to occur, as sequential phosphorylation and dephosphorylation marks the transitions between stages in the splicing process. [20] In addition, hypophosphorylation and hyperphosphorylation of ASF/SF2 by Clk/Sty can lead to inhibition of splicing. [13]

Biological importance

Stability and fidelity

ASF/SF2 is involved in genomic stability; it is thought that RNA Polymerase recruits ASF/SF2 to nascent RNA transcripts to impede formation of mutagenic DNA:RNA hybrid R-loop structures between the transcript and the template DNA. [8] In this way, ASF/SF2 is protecting cells from the potential deleterious effects of transcription itself. [8] ASF/SF2 is also implicated in cellular mechanisms to hinder exon skipping and to ensure splicing is occurring accurately and correctly. [10]

Development and growth

ASF/SF2 has been shown to have a critical function in heart development, [12] embryogenesis, tissue formation, cell motility, and cell viability in general. [21] [22]

Clinical significance

SFRS1 is a proto-oncogene, and thus ASF/SF2 can act as an oncoprotein; it can alter the splicing patterns of crucial cell cycle regulatory genes and suppressor genes. [13] ASF/SF2 controls the splicing of various tumor suppressor genes, kinases, and kinase receptors, all of which have the potential to be alternatively spliced into oncogenic isoforms. [23] As such, ASF/SF2 is an important target for cancer therapy, as it is over-expressed in many tumors. [13]

Modifications and defects in the alternative splicing pathway are associated with a variety of human diseases. [24]

ASF/SF2 is involved in the replication of HIV-1, as HIV-1 needs a delicate balance of spliced and unspliced forms of its viral DNA. [25] ASF/SF2 action in the replication of HIV-1 is a potential target for HIV therapy. [25] ASF/SF2 is also implicated in the production of T cell receptors in Systemic Lupus Erythematosus, altering specific chain expression in T cell receptors through alternative splicing. [26] [27]

Interactions

ASF/SF2 has been shown to interact with:

Related Research Articles

<span class="mw-page-title-main">Alternative splicing</span> Process by which a gene can code for multiple proteins

Alternative splicing, or alternative RNA splicing, or differential splicing, is an alternative splicing process during gene expression that allows a single gene to code for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene. This means the exons are joined in different combinations, leading to different (alternative) mRNA strands. Consequently, the proteins translated from alternatively spliced mRNAs usually contain differences in their amino acid sequence and, often, in their biological functions.

<span class="mw-page-title-main">SR protein</span>

SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are "S" and "R" respectively. SR proteins are ~200-600 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.

<span class="mw-page-title-main">KHDRBS1</span> Protein-coding gene in the species Homo sapiens

KH domain-containing, RNA-binding, signal transduction-associated protein 1 is a protein that in humans is encoded by the KHDRBS1 gene.

<span class="mw-page-title-main">PAK2</span> Mammalian protein found in Homo sapiens

Serine/threonine-protein kinase PAK 2 is an enzyme that in humans is encoded by the PAK2 gene.

<span class="mw-page-title-main">SFRS2</span> Protein-coding gene in humans

Splicing factor, arginine/serine-rich 2 is a protein that in humans is encoded by the SFRS2 gene. MDS-associated splicing factor SRSF2 affects the expression of Class III and Class IV isoforms and perturbs granulopoiesis and SRSF2 P95H promotes Class IV splicing by binding to key ESE sequences in CSF3R exon 17, and that SRSF2, when mutated, contributes to dysgranulopoiesis.

snRNP70 Protein-coding gene in the species Homo sapiens

snRNP70 also known as U1 small nuclear ribonucleoprotein 70 kDa is a protein that in humans is encoded by the SNRNP70 gene. snRNP70 is a small nuclear ribonucleoprotein that associates with U1 spliceosomal RNA, forming the U1snRNP a core component of the spliceosome. The U1-70K protein and other components of the spliceosome complex form detergent-insoluble aggregates in both sporadic and familial human cases of Alzheimer's disease. U1-70K co-localizes with Tau in neurofibrillary tangles in Alzheimer's disease.

<span class="mw-page-title-main">U2 small nuclear RNA auxiliary factor 1</span> Protein-coding gene in the species Homo sapiens

Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.

<span class="mw-page-title-main">SRPK1</span> Protein-coding gene in the species Homo sapiens

Serine/arginine-Rich Splicing Factor (SRSF) protein kinase-1 SRPK1 is an enzyme that in humans is encoded by the SRPK1 gene.

<span class="mw-page-title-main">CDC2L1</span> Protein-coding gene in the species Homo sapiens

PITSLRE serine/threonine-protein kinase CDC2L1 is an enzyme that in humans is encoded by the CDC2L1 gene.

<span class="mw-page-title-main">SFRS3</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 3 is a protein that in humans is encoded by the SFRS3 gene.

<span class="mw-page-title-main">SFRS6</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 6 is a protein that in humans is encoded by the SFRS6 gene.

<span class="mw-page-title-main">SFRS7</span> Protein-coding gene in the species Homo sapiens

Serine/arginine-rich splicing factor 7 (SRSF7) also known as splicing factor, arginine/serine-rich 7 (SFRS7) or splicing factor 9G8 is a protein that in humans is encoded by the SRSF7 gene.

<span class="mw-page-title-main">SFRS9</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 9, also known as SFRS9, is a human gene encoding an SR protein involved in splice site selection in alternative splicing.

<span class="mw-page-title-main">SFRS5</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 5 is a protein that in humans is encoded by the SFRS5 gene.

<span class="mw-page-title-main">CLK1</span> Protein-coding gene in the species Homo sapiens

Dual specificity protein kinase CLK1 is an enzyme that in humans is encoded by the CLK1 gene.

<span class="mw-page-title-main">SFRS4</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 4 is a protein that in humans is encoded by the SFRS4 gene.

<span class="mw-page-title-main">PRPF4B</span> Protein-coding gene in the species Homo sapiens

Serine/threonine-protein kinase PRP4 homolog is an enzyme that in humans is encoded by the PRPF4B gene.

<span class="mw-page-title-main">SRPK2</span> Protein-coding gene in the species Homo sapiens

Serine/threonine-protein kinase SRPK2 is an enzyme that in humans is encoded by the SRPK2 gene.

<span class="mw-page-title-main">SFRS2IP</span> Protein-coding gene in the species Homo sapiens

SFRS2-interacting protein is a protein that in humans is encoded by the SFRS2IP gene.

<span class="mw-page-title-main">SFRS11</span> Protein-coding gene in the species Homo sapiens

Splicing factor, arginine/serine-rich 11 is a protein that in humans is encoded by the SFRS11 gene.

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000136450 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000018379 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ "Entrez Gene: SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor)".
  6. 1 2 Kim DJ, Oh B, Kim YY (Jan 2009). "Splicing factor ASF/SF2 and transcription factor PPAR-gamma cooperate to directly regulate transcription of uncoupling protein-3". Biochemical and Biophysical Research Communications. 378 (4): 877–82. doi:10.1016/j.bbrc.2008.12.009. PMID   19073146.
  7. 1 2 Zuo P, Manley JL (Dec 1993). "Functional domains of the human splicing factor ASF/SF2". The EMBO Journal. 12 (12): 4727–37. doi:10.1002/j.1460-2075.1993.tb06161.x. PMC   413918 . PMID   8223481.
  8. 1 2 3 4 Li X, Manley JL (Aug 2005). "Inactivation of the SR protein splicing factor ASF/SF2 results in genomic instability". Cell. 122 (3): 365–78. doi: 10.1016/j.cell.2005.06.008 . PMID   16096057. S2CID   14126033.
  9. ↑ Bermingham JR, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG (Sep 1995). "Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2)". Genomics. 29 (1): 70–9. doi: 10.1006/geno.1995.1216 . PMID   8530103.
  10. 1 2 3 4 Krainer AR, Conway GC, Kozak D (Jul 1990). "The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites". Cell. 62 (1): 35–42. doi:10.1016/0092-8674(90)90237-9. PMID   2364434. S2CID   29839844.
  11. 1 2 Michlewski G, Sanford JR, Cáceres JF (Apr 2008). "The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1". Molecular Cell. 30 (2): 179–89. doi: 10.1016/j.molcel.2008.03.013 . PMID   18439897.
  12. 1 2 3 4 Ngo JC, Giang K, Chakrabarti S, Ma CT, Huynh N, Hagopian JC, Dorrestein PC, Fu XD, Adams JA, Ghosh G (Mar 2008). "A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1". Molecular Cell. 29 (5): 563–76. doi:10.1016/j.molcel.2007.12.017. PMC   2852395 . PMID   18342604.
  13. 1 2 3 4 5 6 7 8 9 Hagopian JC, Ma CT, Meade BR, Albuquerque CP, Ngo JC, Ghosh G, Jennings PA, Fu XD, Adams JA (Oct 2008). "Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1". Journal of Molecular Biology. 382 (4): 894–909. doi:10.1016/j.jmb.2008.07.055. PMC   2741138 . PMID   18687337.
  14. ↑ Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA (August 2007). "Structural and functional analysis of RNA and TAP binding to SF2/ASF". EMBO Rep. 8 (8): 756–62. doi:10.1038/sj.embor.7401031. PMC   1978082 . PMID   17668007.
  15. ↑ Masuyama K, Taniguchi I, Okawa K, Ohno M (Aug 2007). "Factors associated with a purine-rich exonic splicing enhancer sequence in Xenopus oocyte nucleus". Biochemical and Biophysical Research Communications. 359 (3): 580–5. doi:10.1016/j.bbrc.2007.05.144. PMID   17548051.
  16. 1 2 Zhang X, Merkler KA, McLean MP (Jul 2008). "Characterization of regulatory intronic and exonic sequences involved in alternative splicing of scavenger receptor class B gene". Biochemical and Biophysical Research Communications. 372 (1): 173–8. doi:10.1016/j.bbrc.2008.05.007. PMID   18477479.
  17. ↑ Wang Z, Xiao X, Van Nostrand E, Burge CB (Jul 2006). "General and specific functions of exonic splicing silencers in splicing control". Molecular Cell. 23 (1): 61–70. doi:10.1016/j.molcel.2006.05.018. PMC   1839040 . PMID   16797197.
  18. 1 2 3 Ma CT, Velazquez-Dones A, Hagopian JC, Ghosh G, Fu XD, Adams JA (Feb 2008). "Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1". Journal of Molecular Biology. 376 (1): 55–68. doi:10.1016/j.jmb.2007.08.029. PMID   18155240.
  19. 1 2 Sato H, Hosoda N, Maquat LE (Feb 2008). "Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay". Molecular Cell. 29 (2): 255–62. doi: 10.1016/j.molcel.2007.12.009 . PMID   18243119.
  20. ↑ Cao W, Jamison SF, Garcia-Blanco MA (Dec 1997). "Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro". RNA. 3 (12): 1456–67. PMC   1369586 . PMID   9404896.
  21. ↑ Ghigna C, Giordano S, Shen H, Benvenuto F, Castiglioni F, Comoglio PM, Green MR, Riva S, Biamonti G (Dec 2005). "Cell motility is controlled by SF2/ASF through alternative splicing of the Ron protooncogene". Molecular Cell. 20 (6): 881–90. doi: 10.1016/j.molcel.2005.10.026 . PMID   16364913.
  22. ↑ Lin S, Xiao R, Sun P, Xu X, Fu XD (Nov 2005). "Dephosphorylation-dependent sorting of SR splicing factors during mRNP maturation". Molecular Cell. 20 (3): 413–25. doi: 10.1016/j.molcel.2005.09.015 . PMID   16285923.
  23. ↑ Karni R, de Stanchina E, Lowe SW, Sinha R, Mu D, Krainer AR (Mar 2007). "The gene encoding the splicing factor SF2/ASF is a proto-oncogene". Nature Structural & Molecular Biology. 14 (3): 185–93. doi:10.1038/nsmb1209. PMC   4595851 . PMID   17310252.
  24. ↑ Watanuki T, Funato H, Uchida S, Matsubara T, Kobayashi A, Wakabayashi Y, Otsuki K, Nishida A, Watanabe Y (Sep 2008). "Increased expression of splicing factor SRp20 mRNA in bipolar disorder patients". Journal of Affective Disorders. 110 (1–2): 62–9. doi:10.1016/j.jad.2008.01.003. PMID   18281098.
  25. 1 2 Tange TØ, Kjems J (Sep 2001). "SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain". Journal of Molecular Biology. 312 (4): 649–62. doi:10.1006/jmbi.2001.4971. PMID   11575921.
  26. ↑ Moulton V, Perl M, Tsokos G (2008). "Alternative splicing factor/splicing factor 2 (ASF/SF2) regulates the expression of T cell receptor ζ chain". Clinical Immunology. 127: S95. doi:10.1016/j.clim.2008.03.266.
  27. ↑ Moulton VR, Grammatikos AP, Fitzgerald LM, Tsokos GC (Jan 2013). "Splicing factor SF2/ASF rescues IL-2 production in T cells from systemic lupus erythematosus patients by activating IL-2 transcription". Proceedings of the National Academy of Sciences of the United States of America. 110 (5): 1845–50. Bibcode:2013PNAS..110.1845M. doi: 10.1073/pnas.1214207110 . PMC   3562779 . PMID   23319613.
  28. ↑ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC   305846 . PMID   11101529.
  29. ↑ Colwill K, Feng LL, Yeakley JM, Gish GD, Cáceres JF, Pawson T, Fu XD (Oct 1996). "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors". The Journal of Biological Chemistry. 271 (40): 24569–75. doi: 10.1074/jbc.271.40.24569 . PMID   8798720.
  30. 1 2 3 Umehara H, Nishii Y, Morishima M, Kakehi Y, Kioka N, Amachi T, Koizumi J, Hagiwara M, Ueda K (Feb 2003). "Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A". Biochemical and Biophysical Research Communications. 301 (2): 324–9. doi:10.1016/s0006-291x(02)03017-6. PMID   12565863.
  31. ↑ Ge H, Si Y, Wolffe AP (Dec 1998). "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2". Molecular Cell. 2 (6): 751–9. doi: 10.1016/s1097-2765(00)80290-7 . PMID   9885563.
  32. 1 2 Zhang WJ, Wu JY (Oct 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Molecular and Cellular Biology. 16 (10): 5400–8. doi:10.1128/MCB.16.10.5400. PMC   231539 . PMID   8816452.
  33. ↑ Lukasiewicz R, Velazquez-Dones A, Huynh N, Hagopian J, Fu XD, Adams J, Ghosh G (Aug 2007). "Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions". The Journal of Biological Chemistry. 282 (32): 23036–43. doi: 10.1074/jbc.M611305200 . PMID   17517895.
  34. 1 2 Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD (Feb 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". The Journal of Cell Biology. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC   2141757 . PMID   9472028.
  35. 1 2 Koizumi J, Okamoto Y, Onogi H, Mayeda A, Krainer AR, Hagiwara M (Apr 1999). "The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs)". The Journal of Biological Chemistry. 274 (16): 11125–31. doi: 10.1074/jbc.274.16.11125 . PMID   10196197.
  36. ↑ Labourier E, Rossi F, Gallouzi IE, Allemand E, Divita G, Tazi J (Jun 1998). "Interaction between the N-terminal domain of human DNA topoisomerase I and the arginine-serine domain of its substrate determines phosphorylation of SF2/ASF splicing factor". Nucleic Acids Research. 26 (12): 2955–62. doi:10.1093/nar/26.12.2955. PMC   147637 . PMID   9611241.
  37. ↑ Andersen FF, Tange TØ, Sinnathamby T, Olesen JR, Andersen KE, Westergaard O, Kjems J, Knudsen BR (Sep 2002). "The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation". Journal of Molecular Biology. 322 (4): 677–86. doi:10.1016/s0022-2836(02)00815-x. PMID   12270705.
  38. 1 2 Xiao SH, Manley JL (Nov 1998). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2". The EMBO Journal. 17 (21): 6359–67. doi:10.1093/emboj/17.21.6359. PMC   1170960 . PMID   9799243.
  39. ↑ Cao W, Garcia-Blanco MA (Aug 1998). "A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding". The Journal of Biological Chemistry. 273 (32): 20629–35. doi: 10.1074/jbc.273.32.20629 . PMID   9685421.
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