Dmitry Bandura is a Soviet-born Canadian scientist, notable for being one of the co-inventors of the Mass cytometry technology. [1] [2] Bandura co-founded DVS Sciences [3] in 2004 (acquired by Fluidigm in 2014 and then renamed to Standard BioTools [4] in 2022) along with Drs Vladimir Baranov, Scott D. Tanner, and Olga Ornatsky.
Bandura grew up in Chernivtsi, Ukraine, where he graduated from school #35 with distinction. He received an MSc in engineering physics in 1985 and a PhD in technical sciences, both supervised by Professor Alexander A. Sysoev [5] [6] at Moscow Engineering Physics Institute. His PhD thesis research focused on elemental analysis of hypervelocity microparticles via time-of-flight mass spectrometry (TOF-MS) of their impact-induced plasma. [5] [7]
Bandura emigrated to Australia in 1992, where he worked as a Research Physicist at GBC Scientific Equipment. [8] There, he worked on the development of inductively coupled plasma mass spectrometry (ICP-MS), [7] contributing to the release of the award-winning Optimass 8000 ICP-TOF-MS in 1998. [8] Bandura then relocated to Toronto, Canada, where he joined MDS SCIEX (now Sciex) to continue working on the development of new ICP-MS instrumentation methods, particularly in the area of collision and reaction cells. [9]
In 2005, together with Scott D. Tanner and Vladimir Baranov, Bandura began independently developing an ICP-TOF-MS based cytometer and became a researcher at the University of Toronto in March 2005. [10] After securing ample funding by 2010 from various sources, including National Institutes of Health, Ontario Institute for Cancer Research (OICR), the Ministry of Research and Innovation, Ontario Centres of Excellence, Health Technology Exchange, and Genome Canada via the Ontario Genomics Institute, [11] [12] [3] and venture capital from 5 AM Ventures, [13] Bandura and the DVS Sciences team successfully commercialized their technology, leading to the acquisition of DVS Sciences by Fluidigm in 2014 [14]
Bandura headed R&D and Canadian operations at Fluidigm Canada following the merger and Standard BioTools Canada (formerly DVS Sciences) following a capital infusion in 2022, [15] stewarding the development of the next generation of mass cytometry and imaging mass cytometry instruments and reagents.
A more complete listing of his publications can be found on Google scholar
Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry that uses an inductively coupled plasma to ionize the sample. It atomizes the sample and creates atomic and small polyatomic ions, which are then detected. It is known and used for its ability to detect metals and several non-metals in liquid samples at very low concentrations. It can detect different isotopes of the same element, which makes it a versatile tool in isotopic labeling.
Environmental chemistry is the scientific study of the chemical and biochemical phenomena that occur in natural places. It should not be confused with green chemistry, which seeks to reduce potential pollution at its source. It can be defined as the study of the sources, reactions, transport, effects, and fates of chemical species in the air, soil, and water environments; and the effect of human activity and biological activity on these. Environmental chemistry is an interdisciplinary science that includes atmospheric, aquatic and soil chemistry, as well as heavily relying on analytical chemistry and being related to environmental and other areas of science.
Gold fingerprinting is a method of identifying an item made of gold based on the impurities or trace elements it contains.
Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass analyzer are performed with an additional reaction step in between these analyses to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides.
An inductively coupled plasma (ICP) or transformer coupled plasma (TCP) is a type of plasma source in which the energy is supplied by electric currents which are produced by electromagnetic induction, that is, by time-varying magnetic fields.
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy-absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules and various organic molecules, which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions.
In a chemical analysis, the internal standard method involves adding the same amount of a chemical substance to each sample and calibration solution. The internal standard responds proportionally to changes in the analyte and provides a similar, but not identical, measurement signal. It must also be absent from the sample matrix to ensure there is no other source of the internal standard present. Taking the ratio of analyte signal to internal standard signal and plotting it against the analyte concentrations in the calibration solutions will result in a calibration curve. The calibration curve can then be used to calculate the analyte concentration in an unknown sample.
Time-of-flight mass spectrometry (TOFMS) is a method of mass spectrometry in which an ion's mass-to-charge ratio is determined by a time of flight measurement. Ions are accelerated by an electric field of known strength. This acceleration results in an ion having the same kinetic energy as any other ion that has the same charge. The velocity of the ion depends on the mass-to-charge ratio. The time that it subsequently takes for the ion to reach a detector at a known distance is measured. This time will depend on the velocity of the ion, and therefore is a measure of its mass-to-charge ratio. From this ratio and known experimental parameters, one can identify the ion.
Velmer A. Fassel was an American chemist who developed the inductively coupled plasma (ICP) and demonstrated its use as ion source for mass spectrometry.
Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.
A triple quadrupole mass spectrometer (TQMS), is a tandem mass spectrometer consisting of two quadrupole mass analyzers in series, with a (non-mass-resolving) radio frequency (RF)–only quadrupole between them to act as a cell for collision-induced dissociation. This configuration is often abbreviated QqQ, here Q1q2Q3.
A collision/reaction cell is a device used in inductively coupled plasma mass spectrometry to remove interfering ions through ion/neutral reactions.
Cytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the quantification of multiple cellular components simultaneously using an ICP-MS detector.
Mass spectrometric immunoassay (MSIA) is a rapid method is used to detect and/ or quantify antigens and or antibody analytes. This method uses an analyte affinity isolation to extract targeted molecules and internal standards from biological fluid in preparation for matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). This method allows for "top down" and "bottom up" analysis. This sensitive method allows for a new and improved process for detecting multiple antigens and antibodies in a single assay. This assay is also capable of distinguishing mass shifted forms of the same molecule via a panantibody, as well as distinguish point mutations in proteins. Each specific form is detected uniquely based on their characteristic molecular mass. MSIA has dual specificity because of the antibody-antigen reaction coupled with the power of a mass spectrometer.
Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry). In this approach, antibodies are conjugated with isotopically pure elements, and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argon plasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in flow cytometry by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra.
Standard BioTools Inc., previously known as Fluidigm Corp., provides an established portfolio of essential, standardized technologies that empower customers to scale and accelerate their life sciences research.
Vladimir Baranov is a Soviet born Canadian scientist and one of the original co-inventors of Mass cytometry technology...
Scott Tanner is a Canadian scientist, inventor, and entrepreneur. His areas of expertise include mass spectroscopy, especially inductively coupled plasma mass spectrometry (ICP-MS), and mass cytometry.
Olga Ornatsky is a Soviet born, Canadian scientist. Ornatsky co-founded DVS Sciences in 2004 along with Dmitry Bandura, Vladimir Baranov and Scott D. Tanner.
