MCM3

Last updated
MCM3
Identifiers
Aliases MCM3 , HCC5, P1-P1.h, RLFB, minichromosome maintenance complex component 3
External IDs OMIM: 602693 MGI: 101845 HomoloGene: 1791 GeneCards: MCM3
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_002388
NM_001270472

NM_008563

RefSeq (protein)

NP_032589

Location (UCSC) Chr 6: 52.26 – 52.28 Mb Chr 1: 20.87 – 20.89 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene. [5]

Function

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [6]

Interactions

MCM3 has been shown to interact with:

See also

Related Research Articles

<span class="mw-page-title-main">MCM6</span>

DNA replication licensing factor MCM6 is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication.

<span class="mw-page-title-main">Cyclin-dependent kinase 1</span> Mammalian protein found in Homo sapiens

Cyclin-dependent kinase 1 also known as CDK1 or cell division cycle protein 2 homolog is a highly conserved protein that functions as a serine/threonine protein kinase, and is a key player in cell cycle regulation. It has been highly studied in the budding yeast S. cerevisiae, and the fission yeast S. pombe, where it is encoded by genes cdc28 and cdc2, respectively. With its cyclin partners, Cdk1 forms complexes that phosphorylate a variety of target substrates ; phosphorylation of these proteins leads to cell cycle progression.

<span class="mw-page-title-main">Replication protein A1</span> Protein-coding gene in the species Homo sapiens

Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.

<span class="mw-page-title-main">MCM7</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.

<span class="mw-page-title-main">MCM2</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene.

<span class="mw-page-title-main">CDC6</span> Protein-coding gene in the species Homo sapiens

Cell division control protein 6 homolog is a protein that in humans is encoded by the CDC6 gene.

<span class="mw-page-title-main">MCM4</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene.

<span class="mw-page-title-main">RAD1 homolog</span> Protein-coding gene in the species Homo sapiens

Cell cycle checkpoint protein RAD1 is a protein that in humans is encoded by the RAD1 gene.

<span class="mw-page-title-main">DNA replication factor CDT1</span> Protein-coding gene in the species Homo sapiens

CDT1 is a protein that in humans is encoded by the CDT1 gene. It is a licensing factor that functions to limit DNA from replicating more than once per cell cycle.

<span class="mw-page-title-main">ORC2</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 2 is a protein that is encoded by the ORC2 (ORC2L) gene in humans.

<span class="mw-page-title-main">MCM5</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene.

<span class="mw-page-title-main">Cell division cycle 7-related protein kinase</span> Protein-coding gene in the species Homo sapiens

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The gene CDC7 appears to be conserved throughout eukaryotic evolution; this means that most eukaryotic cells have the Cdc7 kinase protein.

<span class="mw-page-title-main">ORC4</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 4 is a protein that in humans is encoded by the ORC4 (ORC4L) gene.

<span class="mw-page-title-main">ORC6</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 6 is a protein that in humans is encoded by the ORC6 (ORC6L) gene.

<span class="mw-page-title-main">MCM10</span> Protein-coding gene in the species Homo sapiens

Protein MCM10 homolog is a protein that in humans is encoded by the MCM10 gene. It is essential for activation of the Cdc45:Mcm2-7:GINS helicase, and thus required for proper DNA replication.

<span class="mw-page-title-main">CDC45-related protein</span> Protein-coding gene in the species Homo sapiens

CDC45 is a protein that in humans is encoded by the CDC45L gene.

<span class="mw-page-title-main">MCM3AP</span> Protein-coding gene in the species Homo sapiens

80 kDa MCM3-associated protein is a protein that in humans is encoded by the MCM3AP gene.

<span class="mw-page-title-main">ORC3</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3 (ORC3L) gene.

<span class="mw-page-title-main">CDC14A</span> Protein-coding gene in the species Homo sapiens

Dual specificity protein phosphatase CDC14A is an enzyme that in humans is encoded by the CDC14A gene.

<span class="mw-page-title-main">ORC1</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 1 is a protein that in humans is encoded by the ORC1 gene. It is closely related to CDC6, and both are the same protein in archaea.

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000112118 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000041859 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Thömmes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (April 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res. 20 (5): 1069–74. doi:10.1093/nar/20.5.1069. PMC   312092 . PMID   1549468.
  6. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)".
  7. 1 2 3 4 5 6 7 Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID   12614612.
  8. 1 2 Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T (September 1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi: 10.1074/jbc.274.36.25927 . PMID   10464337.
  9. Méndez J, Stillman B (November 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. 20 (22): 8602–12. doi:10.1128/mcb.20.22.8602-8612.2000. PMC   102165 . PMID   11046155.
  10. Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi: 10.1074/jbc.C200442200 . PMID   12226073.
  11. Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E, Kouno Y, Tomiyasu S, Fujimura S, Tokuhisa T, Kimura H, Ezaki T, Sakaguchi N (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood. 95 (7): 2321–8. doi:10.1182/blood.V95.7.2321. PMID   10733502.
  12. Takei Y, Tsujimoto G (August 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi: 10.1074/jbc.273.35.22177 . PMID   9712829.
  13. DaFonseca CJ, Shu F, Zhang JJ (March 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. Bibcode:2001PNAS...98.3034D. doi: 10.1073/pnas.061487598 . PMC   30602 . PMID   11248027.
  14. Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi: 10.1074/jbc.272.16.10928 . PMID   9099751.
  15. Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (March 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. 277 (12): 10354–61. doi: 10.1074/jbc.M111398200 . PMID   11779870.

Further reading