Mitochondrial membrane transport protein

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Depiction of mitochondrial membranes. Mitochondrion structure.svg
Depiction of mitochondrial membranes.

Mitochondrial membrane transport proteins, also known as mitochondrial carrier proteins, are proteins which exist in the membranes of mitochondria. They serve to transport [2] molecules and other factors, such as ions, into or out of the organelles. Mitochondria contain both an inner and outer membrane, separated by the inter-membrane space, or inner boundary membrane. The outer membrane is porous, whereas the inner membrane restricts the movement of all molecules. The two membranes also vary in membrane potential and pH. [3] These factors play a role in the function of mitochondrial membrane transport proteins. There are 53 discovered human mitochondrial membrane transporters, [4] with many others that are known to still need discovered.

Contents

Mitochondrial outer membrane

The outer mitochondrial membrane forms the border of mitochondria towards the cellular environment. The outer membrane mitochondrial proteins carry out functions for mitochondrial biogenesis and integration between mitochondria and the cellular system. The outer membrane consists of two types of integral proteins, including proteins with transmembrane β-barrel and proteins with one or more α-helical membrane anchors. [5] [6]

β-Barrel Outer Membrane Proteins

TOM complex

The TOM complex, part of the TOM/TIM supercomplex, is essential for the translocase of almost all mitochondrial proteins which consists of at least 7 different subunits. Tom20 and Tom70 are the primary receptors while Tom40, Tom22, Tom7, Tom6, and Tom5 subunits form the stable TOM Complex. [7] [8] [9] The receptor proteins Tom70 and Tom20 recognize incoming precursor proteins, in which Tom70 is responsible for docking of precursors of hydrophobic proteins accompanied by cytosolic chaperones and Tom 20 recognizes precursor proteins of the presequence pathways. [10] [11] [12] [13] [14] [15] [16] Tom40 is the protein-conducting channel of the complex with beta-barrel structure, [17] [18] which forms a cation-selective channel. Tom40 has a large pore diameter of 22Å that can allow the accommodation of partially folded protein structure [19] The inner wall of Tom40 has a charged region that allows interaction with hydrophilic precursor proteins while the hydrophobic precursor of ADP/ATP carrier can be crosslinked with the hydrophobic region of Tom40. Three small proteins Tom5, Tom6, Tom7 interact closely with Tom40 to assemble and stabilize the complex. The TOM complex also consists of a dimer of Tom40 or small Tom proteins that are held together by two Tom22 subunits. [20] [21] Protein sorting into the mitochondrial compartments always starts at the TOM complex. The TOM complex forms two exit sites for precursor proteins—Tom40, Tom7, and the intermembrane space domain of Tom22—promote the transfer of presequence-containing precursors to the TIM23 complex. [20]

SAM complex

The SAM Complex is essential for sorting and assembling beta-barrel proteins from the intermembrane space side into the outer membrane. [22] [23] [24] The SAM complex consists of three subunits: The β-barrel protein Sam50 and two peripheral subunits Sam35 and Sam37. [22] [25] [26] Sam50 belongs to the conserved Omp85 protein family which can be characterized by a 16-stranded β-barrel and by a different number of polypeptide transport-associated (POTRA) domains. [23] [24] Sam50 exposes a single POTRA domain towards the intermembrane space. [26] [27] Sam35 caps the Sam50 β-barrel, stabilizing the core of the protein translocase. [26] [28] [29] Sam50 and Sam35 are responsible for the binding of precursors of β-barrel proteins, which contain conserved β-signal that is formed by the last β-strand. [30] [31] The β-barrel of Sam50 is the functional domain that inserts and folds substrate proteins into the outer membrane.

Sam35 binds to Sam50 and closely interacts with Sam37, in which Sam37 does not bind to Sam50. Sam37 and Sam35 have a conformation similar to glutathione-S-transferase, except they do not possess residues required for enzymatic activity. Sam37 accommodates the release of the folded β-barrel proteins from the SAM complex. [31]

Voltage-dependent anion ion channel or VDAC

VDAC (voltage-dependent anion ion channel) is important for the exchange of small hydrophilic ions and metabolites with the cytosol, which is driven by the gradient concentration across the outer membrane. VDAC is the most abundant protein in the outer membrane. [32] [33] Like Tom40, VDAC has a β-barrel structure with antiparallel β-strands that can facilitate the passage of β-barrel membrane proteins. VDAC has a pore size of 2-4 nm for small hydrophilic molecules. VDAC plays a crucial role in facilitating energy metabolism by transporting ADP and ATP in and out of the outer membrane. VDAC also accommodates the passage of NADH and many anionic metabolites. VDAC operation is voltage-dependent in which it closes at high voltage and can partially open towards slightly reduced anion selectivity. [34] [35]

α-Helical outer membrane proteins

The Mitochondrial import complex (MIM)

The import pathways of α-helical membrane anchors or signal-anchored proteins are carried out mainly by outer membrane proteins. [6] Precursors of the polytopic or multi-spanning proteins can be recognized by Tom70, but cannot be passed through the Tom40 channel. [12] [36] [37] Tom70 transfers the precursor proteins to the MIM Complex. The MIM complex constitutes the major inserts for alpha-helical proteins into the target membrane. [12] [13] [37] The MIM Complex consists of several copies of Mim1 and one or two copies of Mim2. Both subunits are necessary for stabilizing partner proteins and for outer membrane protein biogenesis [38]

Mitochondrial inner membrane

The inner mitochondrial membrane is a structure that surrounds the mitochondrial matrix, characterized by many folds and compartments that form crista and is the site of oxidative phosphorylation and ATP synthesis. [3] [39] The high concentration of cardiolipin, a type of lipid and about 20% of the inner membrane composition, makes it impermeable to most molecules. Specialized transporters arranged in specific configurations are required to regulate the diffusion of molecules across the membrane. The inner membrane's structure causes a membrane potential of approximately 180 mV. [39]

Respiratory chain supercomplex

Respiratory chain supercomplex components. Respiratory Chain supercomplex.jpg
Respiratory chain supercomplex components.

The respiratory chain supercomplex is located in the cristae of the inner membrane. It is composed of multiple complexes that work together to drive oxidative phosphorylation and ATP synthesis. The complexes cannot function without the other parts of the respiratory supercomplex being present. [39] The supercomplex is the site of the mitochondrial electron transport chain. [40]

NADH/ubiquinone oxidoreductase

NADH/ubiquinone oxidoreductase, also known as complex I, is the first and largest protein in the mitochondrial respiratory chain. It consists of a membrane arm, embedded inside the inner mitochondrial membrane, and a matrix arm, extending out of the membrane. There are 78 transmembrane helices and three proton pumps. The junction of the two arms is the site of conduction of NADH to ubiquinol. [39] Complex I is a scaffold needed for complex III and IV, and it will not function without these other complexes being present. [40]

Cytochrome c reductase, succinate dehydrogenase, and cytochrome c oxidase

Cytochrome c reductase, also known as complex III, is the second protein in the respiratory chain. It pumps electrons from complex I, through succinate dehydrogenase (complex II) to cytochrome c (complex IV). Complex III and IV are proton pumps, pumping H+ protons out of the mitochondrial matrix, and work in conjunction with complex I to create the proton gradient found at the inner membrane. Cytochrome c is and electron carrier protein that travels between complex III and IV, and triggers apoptosis if it leaves the cristae. Complex IV passes electrons to oxygen, the final acceptor in the mitochondrial electron transport chain. [40] [3]

Inner membrane translocases

TIM complex

The TIM complex is a protein translocase located on the inner membrane. It is part of the TOM/TIM supercomplex, which spans the intermembrane space. [3] The TIM complex is responsible for sorting proteins into the mitochondrial matrix or into the membrane. TIM22 and TIM23 are the main subunits. TIM 22 is responsible for allowing other mitochondrial transporters to insert themselves into the inner membrane, whereas TIM23 reads proteins with an N-terminus precursor for import into the membrane or matrix. [41]

ADP, ATP translocase

ADP, ATP translocase is responsible for regulating the movement of ADP and ATP in and out of the inner membrane. ATP is sorted into the cytosol, while ADP is sorted into the mitochondrial matrix to undergo oxidative phosphorylation. Due to the constant demand of ATP production, ADP, ATP translocases are in higher abundance than other transporters. [42] [43] ADP, ATP translocase is a small protein, ~30-33 kDa, composed of 6 transmembrane α-helices, that form 3 repeat domains for an overall funnel-like structure in the membrane. Towards the center of the funnel structure it has a 7 amino acid loop 12. It is structurally unique when compared to other proteins that interact with ATP in that it lacks adenosine monophosphate and requires at least two phosphate groups to allow for passage of the molecule. It's composed of 297 amino acid residues, with 18 of them being charged molecules. The ADP, ATP translocase is opened in the presence of Ca2+. [43] [39]

Phosphate transport proteins

Phosphate transport proteins are similar in structure and are both part of the same family of mitochondrial carriers. It consists of 6 transmembrane α-helices, but lacks the 7 amino acid loop 12 found in ADP, ATP translocase. Phosphate transport proteins are responsible for transport of phosphate across the inner membrane so it can be used in the phosphorylation of ADP. [39]

Mutations of mitochondrial membrane transporters

Mutations of DNA coding for mitochondrial membrane transport proteins are linked to a wide range of diseases and disorders, such as cardiomyopathy, encephalopathy, muscular dystrophy, epilepsy, neuropathy, and fingernail dysplasia. [44] Most mutations of mitochondrial membrane transporters are autosomal recessive. Mutations to transporters within the inner mitochondrial membrane mostly affect high-energy tissues due to the disruption of oxidative phosphorylation. [4] [44] For example, decreased mitochondrial function has been linked to heart failure and hypertrophy. This mitochondrial response translates into a shift towards glycolysis and lactate production that can cause tumor formation and proliferation of the tissues. [40]

Examples

Examples of mitochondrial transport proteins include the following:

See also

Related Research Articles

<span class="mw-page-title-main">Mitochondrion</span> Organelle in eukaryotic cells responsible for respiration

A mitochondrion is an organelle found in the cells of most eukaryotes, such as animals, plants and fungi. Mitochondria have a double membrane structure and use aerobic respiration to generate adenosine triphosphate (ATP), which is used throughout the cell as a source of chemical energy. They were discovered by Albert von Kölliker in 1857 in the voluntary muscles of insects. The term mitochondrion was coined by Carl Benda in 1898. The mitochondrion is popularly nicknamed the "powerhouse of the cell", a phrase coined by Philip Siekevitz in a 1957 article of the same name.

Protein targeting or protein sorting is the biological mechanism by which proteins are transported to their appropriate destinations within or outside the cell. Proteins can be targeted to the inner space of an organelle, different intracellular membranes, the plasma membrane, or to the exterior of the cell via secretion. Information contained in the protein itself directs this delivery process. Correct sorting is crucial for the cell; errors or dysfunction in sorting have been linked to multiple diseases.

<span class="mw-page-title-main">Intermembrane space</span>

The intermembrane space (IMS) is the space occurring between or involving two or more membranes. In cell biology, it is most commonly described as the region between the inner membrane and the outer membrane of a mitochondrion or a chloroplast. It also refers to the space between the inner and outer nuclear membranes of the nuclear envelope, but is often called the perinuclear space. The IMS of mitochondria plays a crucial role in coordinating a variety of cellular activities, such as regulation of respiration and metabolic functions. Unlike the IMS of the mitochondria, the IMS of the chloroplast does not seem to have any obvious function.

<span class="mw-page-title-main">Inner mitochondrial membrane</span>

The inner mitochondrial membrane (IMM) is the mitochondrial membrane which separates the mitochondrial matrix from the intermembrane space.

<span class="mw-page-title-main">TIM/TOM complex</span>

The TIM/TOM complex is a protein complex in cellular biochemistry which translocates proteins produced from nuclear DNA through the mitochondrial membrane for use in oxidative phosphorylation. In enzymology, the complex is described as an mitochondrial protein-transporting ATPase, or more systematically ATP phosphohydrolase , as the TIM part requires ATP hydrolysis to work.

<span class="mw-page-title-main">Adenine nucleotide translocator</span> Class of transport proteins

Adenine nucleotide translocator (ANT), also known as the ADP/ATP translocase (ANT), ADP/ATP carrier protein (AAC) or mitochondrial ADP/ATP carrier, exchanges free ATP with free ADP across the inner mitochondrial membrane. ANT is the most abundant protein in the inner mitochondrial membrane and belongs to mitochondrial carrier family.

<span class="mw-page-title-main">Mitochondrial carrier</span>

Mitochondrial carriers are proteins from solute carrier family 25 which transfer molecules across the membranes of the mitochondria. Mitochondrial carriers are also classified in the Transporter Classification Database. The Mitochondrial Carrier (MC) Superfamily has been expanded to include both the original Mitochondrial Carrier (MC) family and the Mitochondrial Inner/Outer Membrane Fusion (MMF) family.

Translocase is a general term for a protein that assists in moving another molecule, usually across a cell membrane. These enzymes catalyze the movement of ions or molecules across membranes or their separation within membranes. The reaction is designated as a transfer from “side 1” to “side 2” because the designations “in” and “out”, which had previously been used, can be ambiguous. Translocases are the most common secretion system in Gram positive bacteria.

<span class="mw-page-title-main">Voltage-dependent anion channel</span> Class of porin ion channels in the outer mitochondrial membrane

Voltage-dependent anion channels, or mitochondrial porins, are a class of porin ion channel located on the outer mitochondrial membrane. There is debate as to whether or not this channel is expressed in the cell surface membrane.

<span class="mw-page-title-main">TOMM20</span> Protein-coding gene in the species Homo sapiens

Mitochondrial import receptor subunit TOM20 homolog is a protein that in humans is encoded by the TOMM20 gene. TOM20 is one of the receptor systems of the TOM complex in the outer mitochondrial membrane (OMM).

<span class="mw-page-title-main">TOMM22</span> Protein-coding gene in the species Homo sapiens

Mitochondrial import receptor subunit TOM22 homolog is a protein that in humans is encoded by the TOMM22 gene.

<span class="mw-page-title-main">Translocase of the outer membrane</span>

The translocase of the outer membrane (TOM) is a complex of proteins found in the outer mitochondrial membrane of the mitochondria. It allows movement of proteins through this barrier and into the intermembrane space of the mitochondrion. Most of the proteins needed for mitochondrial function are encoded by the nucleus of the cell. The outer membrane of the mitochondrion is impermeable to large molecules greater than 5000 daltons. The TOM works in conjunction with the translocase of the inner membrane (TIM) to translocate proteins into the mitochondrion. Many of the proteins in the TOM complex, such as TOMM22, were first identified in Neurospora crassa and Saccharomyces cerevisiae. Many of the genes encoding these proteins are designated as TOMM (translocase of the outer mitochondrial membrane) complex genes.

<span class="mw-page-title-main">VDAC1</span> Protein-coding gene in the species Homo sapiens

Voltage-dependent anion-selective channel 1 (VDAC-1) is a beta barrel protein that in humans is encoded by the VDAC1 gene located on chromosome 5. It forms an ion channel in the outer mitochondrial membrane (OMM) and also the outer cell membrane. In the OMM, it allows ATP to diffuse out of the mitochondria into the cytoplasm. In the cell membrane, it is involved in volume regulation. Within all eukaryotic cells, mitochondria are responsible for synthesis of ATP among other metabolite needed for cell survival. VDAC1 therefore allows for communication between the mitochondrion and the cell mediating the balance between cell metabolism and cell death. Besides metabolic permeation, VDAC1 also acts as a scaffold for proteins such as hexokinase that can in turn regulate metabolism.

<span class="mw-page-title-main">TOMM70A</span> Protein-coding gene in the species Homo sapiens

Mitochondrial import receptor subunit TOM70 is a protein that in humans is encoded by the TOMM70A gene.

<span class="mw-page-title-main">TOMM40</span> Protein-coding gene in the species Homo sapiens

Translocase of outer mitochondrial membrane 40 homolog (yeast), also known as TOMM40, is a protein which in humans is encoded by the TOMM40 gene.

The translocase of the inner membrane (TIM) is a complex of proteins found in the inner mitochondrial membrane of the mitochondria. Components of the TIM complex facilitate the translocation of proteins across the inner membrane and into the mitochondrial matrix. They also facilitate the insertion of proteins into the inner mitochondrial membrane, where they must reside in order to function, these mainly include members of the mitochondrial carrier family of proteins.

Tim9 and Tim10 make up the group of essential small Tim proteins that assist in transport of hydrophobic precursors across the intermembrane space in mammalian cells. Both Tim9 and Tim10 form a hexamer, the Tim9-Tim10 complex, that when associated, functions as a chaperone to assist translocation of preproteins from the outer mitochondrial membrane to the translocase of the inner membrane. The functional Tim9-Tim10 complex not only directs preproteins to the inner mitochondrial membrane in order to interact with the TIM22 complex, but also guides β-barrel precursor proteins to the sorting and assembly machinery (SAM) of the outer membrane.

The outer mitochondrial membrane is made up of two essential proteins, Tom40 and Sam50.

<span class="mw-page-title-main">ADP/ATP translocase 2</span> Protein-coding gene in humans

ADP/ATP translocase 2 is a protein that in humans is encoded by the SLC25A5 gene on the X chromosome.

Carla M. Koehler is an American biochemist who is a professor at the University of California, Los Angeles. Her research considers mitochondria and the processes which import proteins to their appropriate locations in the organelles. She was elected Fellow of the American Association for the Advancement of Science in 2018.

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