The Women's 500m Time Trial was one of the 6 women's events at the 2005 UCI Track Cycling World Championships, held in Los Angeles, United States.
18 Cyclists from 14 countries were due to participate in the contest, Christin Muche of Germany did not start. The Final was held on March 24 at 19:35.
| World Record | ||||
|---|---|---|---|---|
| WR | 33.952 |  Anna Meares (AUS) | Athens  | August 20, 2004 |
| Rank [1] | Name | 250m | Time | Speed (km/h) |
|---|---|---|---|---|
| 250-500 | ||||
 |  Natallia Tsylinskaya (BLR) | 19.453 (2) | 34.738 | 51.816 |
| 15.285 (5) | ||||
 | Anna Meares (AUS) | 19.484 (3) | 34.752 | 51.795 |
| 15.268 (3) | ||||
 |  Yvonne Hijgenaar (NED) | 19.703 (4) | 34.928 | 51.534 |
| 15.225 (2) | ||||
| 4 | Willy Kanis (NED) | 19.233 (1) | 35.056 | 51.346 |
| 15.823 (12) | ||||
| 5 |  Victoria Pendleton (GBR) | 19.815 (5) | 35.088 | 51.299 |
| 15.273 (4) | ||||
| 6 |  Tamilla Abassova (RUS) | 19.954 (8) | 35.109 | 51.268 |
| 15.155 (1) | ||||
| 7 |  Lori-Ann Muenzer (CAN) | 19.824 (6) | 35.217 | 51.111 |
| 15.393 (7) | ||||
| 8 |  Simona Krupeckaitė (LTU) | 19.941 (7) | 35.376 | 50.881 |
| 15.435 (8) | ||||
| 9 |  Elisa Frisoni (ITA) | 20.085 (11) | 35.395 | 50.854 |
| 15.310 (6) | ||||
| 10 |  Céline Nivert (FRA) | 19.996 (9) | 35.628 | 50.522 |
| 15.632 (10) | ||||
| 11 | Clara Sanchez (FRA) | 20.186 (15) | 35.730 | 50.377 |
| 15.544 (9) | ||||
| 12 |  Fang Tian (CHN) | 20.183 (14) | 35.974 | 50.036 |
| 15.791 (11) | ||||
| 13 |  Rebecca Conzelman (USA) | 20.057 (10) | 36.074 | 49.897 |
| 16.017 (14) | ||||
| 14 |  Susann Panzer (GER) | 20.129 (12) | 36.122 | 49.831 |
| 15.993 (13) | ||||
| 15 |  Nancy Contreras (MEX) | 20.159 (13) | 36.343 | 49.528 |
| 16.184 (15) | ||||
| 16 |  Magdalena Sara (POL) | 20.727 (16) | 36.983 | 48.671 |
| 16.256 (16) | ||||
| 17 | Alena Prudnikova (RUS) | 20.908 (17) | 37.497 | 48.003 |
| 16.589 (17) | ||||
| Christin Muche (GER) | 20.353 (16) | DNS |