| Singles | |
|---|---|
| 2016 Jalisco Open | |
| Champion |  Malek Jaziri |
| Runner-up |  Stéphane Robert |
| Final score | 5–7, 6–3, 7–6(7–5) |
Rajeev Ram was the defending champion but chose not to participate.
Malek Jaziri won the title, defeating Stéphane Robert 5–7, 6–3, 7–6(7–5) in the final.
Víctor Estrella Burgos (first round) Lucas Pouille (quarterfinals)
Damir Džumhur (second round)
Roberto Carballés Baena (first round) Malek Jaziri (champion)
Horacio Zeballos (semifinals)
Alejandro Falla (first round)
Daniel Brands (first round)| Semifinals | Final | ||||||||||||
 Alexander Sarkissian | 6 | 65 | 1 | ||||||||||
| Stéphane Robert | 4 | 77 | 6 | ||||||||||
| Stéphane Robert | 7 | 3 | 65 | ||||||||||
| 5 | Malek Jaziri | 5 | 6 | 77 | |||||||||
| 6 | Horacio Zeballos | 77 | 3 | 4 | |||||||||
| 5 | Malek Jaziri | 65 | 6 | 6 | |||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 1 | V Estrella Burgos | 2 | 6 | 5 | |||||||||||||||||||||||
| A Sarkissian | 6 | 2 | 7 | A Sarkissian | 6 | 77 | |||||||||||||||||||||
| WC |  M Sánchez | 62 | 6 | 3 | WC | L Gómez | 1 | 61 | |||||||||||||||||||
| WC | L Gómez | 77 | 4 | 6 | A Sarkissian | 6 | 77 | ||||||||||||||||||||
 J Ward | 7 | 6 | A González | 0 | 64 | ||||||||||||||||||||||
| A Montañés | 5 | 3 | J Ward | 5 | 6 | 4 | |||||||||||||||||||||
| A González | 6 | 4 | 6 | A González | 7 | 2 | 6 | ||||||||||||||||||||
| 7 | A Falla | 3 | 6 | 3 | A Sarkissian | 6 | 65 | 1 | |||||||||||||||||||
| 4 | R Carballés Baena | 2 | 6 | 3 | S Robert | 4 | 77 | 6 | |||||||||||||||||||
| D Novikov | 6 | 4 | 6 | D Novikov | 6 | 6 | |||||||||||||||||||||
| SE | E Struvay | 6 | 6 | SE | E Struvay | 3 | 4 | ||||||||||||||||||||
| WC | H Hach Verdugo | 4 | 2 | D Novikov | 1 | 5 | |||||||||||||||||||||
| Q | E Escobedo | 4 | 2 | S Robert | 6 | 7 | |||||||||||||||||||||
| S Robert | 6 | 6 | S Robert | 6 | 4 | 6 | |||||||||||||||||||||
 J McGee | 6 | 6 | J McGee | 4 | 6 | 4 | |||||||||||||||||||||
| 8 | D Brands | 2 | 2 | ||||||||||||||||||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 6 | H Zeballos | 6 | 77 | ||||||||||||||||||||||||
| WC | T Hank | 3 | 64 | 6 | H Zeballos | 77 | 6 | ||||||||||||||||||||
| M Bourgue | 6 | 4 | 77 | M Bourgue | 64 | 4 | |||||||||||||||||||||
| F Argüello | 3 | 6 | 64 | 6 | H Zeballos | 6 | 3 | 7 | |||||||||||||||||||
| SE |  P Krstin | 78 | 1 | 3 | A Menéndez-Maceiras | 1 | 6 | 5 | |||||||||||||||||||
| A Menéndez-Maceiras | 66 | 6 | 6 | A Menéndez-Maceiras | 1 | 6 | 6 | ||||||||||||||||||||
| Q |  M Arévalo | 2 | 3 | 3 | D Džumhur | 6 | 2 | 0 | |||||||||||||||||||
| 3 | D Džumhur | 6 | 6 | 6 | H Zeballos | 77 | 3 | 4 | |||||||||||||||||||
| 5 | M Jaziri | 6 | 6 | 5 | M Jaziri | 65 | 6 | 6 | |||||||||||||||||||
| Alt | O Roca Batalla | 4 | 4 | 5 | M Jaziri | 6 | 6 | ||||||||||||||||||||
| Q |  M Matosevic | 78 | 6 | Q | M Matosevic | 4 | 3 | ||||||||||||||||||||
| Alt | J Samper-Montaña | 66 | 1 | 5 | M Jaziri | 3 | 6 | 7 | |||||||||||||||||||
| Q | DE Galán | 6 | 78 | 2 | L Pouille | 6 | 3 | 5 | |||||||||||||||||||
 M Donati | 4 | 66 | Q | DE Galán | 3 | 4 | |||||||||||||||||||||
 A Ghem | 3 | 7 | 2 | 2 | L Pouille | 6 | 6 | ||||||||||||||||||||
| 2 | L Pouille | 6 | 5 | 6 | |||||||||||||||||||||||
Andrej Martin was the defending champion, but Radu Albot defeated and eliminated him already in the first round.
Denis Istomin won the title, defeating Malek Jaziri, who retired after Istomin won the first set by a score of 7–6(7–2).
David Guez was the defending champion, but lost in the first round to first seeded Édouard Roger-Vasselin.
Igor Sijsling won the final 6–3, 6–4 against Malek Jaziri.
Dominik Meffert was the defending champion but decided not to participate.
Tatsuma Ito won the title, defeating Malek Jaziri 6–7(5–7), 6–1, 6–2 in the final.
Go Soeda successfully defended his title, defeating Malek Jaziri 6–1, 3–6, 7–5 in the final.
Jo-Wilfried Tsonga defeated Roger Federer in the final, 7–5, 7–6(7–3), to win the men's singles title at the 2014 Canadian Open. Tsonga became the first man to defeat Novak Djokovic, Andy Murray, and Federer in the same tournament since Rafael Nadal at the 2008 Hamburg Masters.
Mikhail Kukushkin was the defending champion, but did not take part this year.
Novak Djokovic was the two-time defending champion, but lost to Roger Federer in the semifinals.
Mikhail Youzhny was the defending champion but lost to Thomaz Bellucci in the first round.
Ken Skupski and Neal Skupski were the defending champions, but decided not to defend their title. Third seeds Saketh Myneni and Divij Sharan won the title defeating Malek Jaziri and Denys Molchanov 7–6(7–5), 4–6, 0–0 retired in the final.
Steve Darcis was the defending champion, but lost in the second round. Malek Jaziri won the title defeating Igor Sijsling in the final 5–7, 7–5, 6–4
Ruben Bemelmans was the defending champion, but decided not to defend his title.
Marin Čilić defeated Andy Murray in the final, 6–4, 7–5, to win the men's singles title at the 2016 Cincinnati Masters. It was his maiden Masters 1000 title, ending a streak of 18 consecutive Masters events won by a member of the Big Four.
Karen Khachanov was the defending champion but chose not to participate.
Thiago Monteiro was the defending champion but chose not to defend his title.
Malek Jaziri was the defending champion and successfully defended his title.
Andy Murray was the defending champion but withdrew with a hip injury before the tournament began.
This was the first edition of the tournament.
Marin Čilić was the defending champion, but lost in the second round to Malek Jaziri.
Roger Federer was the defending champion and successfully defended his title, defeating Marius Copil in the final, 7–6(7–5), 6–4.
Fabio Fognini was the defending champion, but chose not to defend his title.