Cementochronology

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Cementochronology is a method for assessing age at death and determining season at death. This technique is employed as accurate indicator of age among wildlife biologists on present [1] and archaeological [2] populations but is increasingly used in forensic anthropology [3] and physical anthropology. [4]

Contents

Cementochronology is a subdiscipline of the field of study called skeletochronology which is a subdiscipline of the broader field called sclerochronology.

Principle

Cementochronology is based on the assumption that dental cementum deposits reflect an annual rhythm and involves the counting of incremental lines in histological preparations.

This incremental structure has been reported in the dental cementum of marine and terrestrial mammals. [5] When viewed under light microscopy, a specific type of cementum (Acellular Extrinsic Fibers Cementum - AEFC) surrounding the root appears as layers of alternating dark and light bands.

Cementochronology.tif

One pair composed of a single light and a single dark line is considered to represent one calendar year and age estimation is calculated by adding the average age of tooth eruption to the line count determined. The age-at-death of an individual is estimated by adding this count to the average age of tooth eruption.

History

Cementochronology was first used in zoology for estimating both age-at-death and season of death. Then, cementochronology has been successfully implemented in bioarchaeology. In an investigation yet based on nonhuman mammalian, Mina and Klevezal established in human teeth the considerable interest for archaeologists, anthropologists, and forensic pathologists. [6] It took 12 years to reproduce the experiment on other human teeth [7] . Since, this technique has a particular interest for physical and forensic anthropologists because counting cementum deposits would give a direct access to chronological age and some studies performed on cementum have shown a correlation reaching 0.98 between incremental lines in the cementum and known age at death.

Technique

Different protocols are used depending on the field and the period. On present teeth, classical histological methods are often used and require decalcification of the dental tissue and staining protocol for collagen. In archaeology where collagen can be poorly preserved, tooth root is embedded in an epoxy resin and dried in a vacuum chamber. On human teeth, the crown and the upper third of the root are removed. 100-μm, non-decalcified cross sections are prepared from the middle third of the root using a Low Speed Saw fitted with a diamond-coated blade. The polishing of both faces can be performed to remove cutting marks. Each slice is washed and sections are mounted on slides. Observations are conducted using an optical microscope at ×400 magnification. Segments that showed readable cementum layers are captured with a digital camera and readings are performed on selected segments. [3]

Critical evaluation

The destruction of the tooth is the first inconvenient of this technique especially in the fields of archaeology and paleontology where non-invasive techniques are preferred. This technic also suffers from the fact that the physiological and structural biological background of cementum is not well elucidated. The alternating deposits could be regulated under genetic factors but could be influenced by biomechanical and physiological factors in link with nutrition and/or environment.

Nonetheless, studies performed on cementum annulation have shown a strong correlation between the deposits the known age-at-death and allow to estimate adult age-at-death with a better precision than classical dental and osseous methods. [8] Cementochronology narrow down the age at death estimations in zoology, bioarchaeology and forensic sciences. [9]

Related Research Articles

<span class="mw-page-title-main">Human tooth</span> Calcified whitish structure in humans mouths used to break down food

Human teeth function to mechanically break down items of food by cutting and crushing them in preparation for swallowing and digesting. As such, they are considered part of the human digestive system. Humans have four types of teeth: incisors, canines, premolars, and molars, which each have a specific function. The incisors cut the food, the canines tear the food and the molars and premolars crush the food. The roots of teeth are embedded in the maxilla or the mandible and are covered by gums. Teeth are made of multiple tissues of varying density and hardness.

<span class="mw-page-title-main">Cementum</span> Specialized calcified substance covering the root of a tooth

Cementum is a specialized calcified substance covering the root of a tooth. The cementum is the part of the periodontium that attaches the teeth to the alveolar bone by anchoring the periodontal ligament.

<span class="mw-page-title-main">Forensic anthropology</span> Application of the science of anthropology in a legal setting

Forensic anthropology is the application of the anatomical science of anthropology and its various subfields, including forensic archaeology and forensic taphonomy, in a legal setting. A forensic anthropologist can assist in the identification of deceased individuals whose remains are decomposed, burned, mutilated or otherwise unrecognizable, as might happen in a plane crash. Forensic anthropologists are also instrumental in the investigation and documentation of genocide and mass graves. Along with forensic pathologists, forensic dentists, and homicide investigators, forensic anthropologists commonly testify in court as expert witnesses. Using physical markers present on a skeleton, a forensic anthropologist can potentially determine a person's age, sex, stature, and race. In addition to identifying physical characteristics of the individual, forensic anthropologists can use skeletal abnormalities to potentially determine cause of death, past trauma such as broken bones or medical procedures, as well as diseases such as bone cancer.

<span class="mw-page-title-main">Forensic dentistry</span> Aspect of criminal investigation

Forensic dentistry or forensic odontology involves the handling, examination, and evaluation of dental evidence in a criminal justice context. Forensic dentistry is used in both criminal and civil law. Forensic dentists assist investigative agencies in identifying human remains, particularly in cases when identifying information is otherwise scarce or nonexistent—for instance, identifying burn victims by consulting the victim's dental records. Forensic dentists may also be asked to assist in determining the age, race, occupation, previous dental history, and socioeconomic status of unidentified human beings.

<span class="mw-page-title-main">Dentin</span> Calcified tissue of the body; one of the four major components of teeth

Dentin or dentine is a calcified tissue of the body and, along with enamel, cementum, and pulp, is one of the four major components of teeth. It is usually covered by enamel on the crown and cementum on the root and surrounds the entire pulp. By volume, 45% of dentin consists of the mineral hydroxyapatite, 33% is organic material, and 22% is water. Yellow in appearance, it greatly affects the color of a tooth due to the translucency of enamel. Dentin, which is less mineralized and less brittle than enamel, is necessary for the support of enamel. Dentin rates approximately 3 on the Mohs scale of mineral hardness. There are two main characteristics which distinguish dentin from enamel: firstly, dentin forms throughout life; secondly, dentin is sensitive and can become hypersensitive to changes in temperature due to the sensory function of odontoblasts, especially when enamel recedes and dentin channels become exposed.

<span class="mw-page-title-main">Cementoenamel junction</span>

Cementoenamel junction (CEJ) is defined as the area of the union of cementum and enamel at the cervical region of the tooth. It is a slightly visible anatomical border identified on a tooth. It is the location where the enamel, which covers the anatomical crown of a tooth, and the cementum, which covers the anatomical root of a tooth, meet. Informally it is known as the neck of the tooth. The border created by these two dental tissues has much significance as it is usually the location where the gingiva attaches to a healthy tooth by fibers called the gingival fibers.

<span class="mw-page-title-main">Periodontal fiber</span> Group of specialized connective tissue fibers

The periodontal ligament, commonly abbreviated as the PDL, is a group of specialized connective tissue fibers that essentially attach a tooth to the alveolar bone within which it sits. It inserts into root cementum on one side and onto alveolar bone on the other.

The term bioarchaeology has been attributed to British archaeologist Grahame Clark who, in 1972, defined it as the study of animal and human bones from archaeological sites. Redefined in 1977 by Jane Buikstra, bioarchaeology in the United States now refers to the scientific study of human remains from archaeological sites, a discipline known in other countries as osteoarchaeology, osteology or palaeo-osteology. Compared to bioarchaeology, osteoarchaeology is the scientific study that solely focus on the human skeleton. The human skeleton is used to tell us about health, lifestyle, diet, mortality and physique of the past. Furthermore, palaeo-osteology is simple the study of ancient bones.

<span class="mw-page-title-main">Human tooth development</span> Process by which teeth form

Tooth development or odontogenesis is the complex process by which teeth form from embryonic cells, grow, and erupt into the mouth. For human teeth to have a healthy oral environment, all parts of the tooth must develop during appropriate stages of fetal development. Primary (baby) teeth start to form between the sixth and eighth week of prenatal development, and permanent teeth begin to form in the twentieth week. If teeth do not start to develop at or near these times, they will not develop at all, resulting in hypodontia or anodontia.

<span class="mw-page-title-main">Dental follicle</span>

The dental follicle, also known as dental sac, is made up of mesenchymal cells and fibres surrounding the enamel organ and dental papilla of a developing tooth. It is a vascular fibrous sac containing the developing tooth and its odontogenic organ. The dental follicle (DF) differentiates into the periodontal ligament. In addition, it may be the precursor of other cells of the periodontium, including osteoblasts, cementoblasts and fibroblasts. They develop into the alveolar bone, the cementum with Sharpey's fibers and the periodontal ligament fibers respectively. Similar to dental papilla, the dental follicle provides nutrition to the enamel organ and dental papilla and also have an extremely rich blood supply.

Cementogenesis is the formation of cementum, one of the three mineralized substances of a tooth. Cementum covers the roots of teeth and serves to anchor gingival and periodontal fibers of the periodontal ligament by the fibers to the alveolar bone.

Hypercementosis is an idiopathic, non-neoplastic condition characterized by the excessive buildup of normal cementum on the roots of one or more teeth. A thicker layer of cementum can give the tooth an enlarged appearance, which mainly occurs at the apex or apices of the tooth.

<span class="mw-page-title-main">Odontometrics</span> Measurement and study of tooth size

Odontometrics is the measurement and study of tooth size. It is used in biological anthropology and bioarchaeology to study human phenotypic variation. The rationale for use is similar to that of the study of dentition, the structure and arrangement of teeth. There are a number of features that can be observed in human teeth through the use of odontometrics.

Tooth ankylosis refers to a fusion between a tooth and underlying bony support tissues. In some species, this is a normal process that occurs during the formation or maintenance of the dentition. By contrast, in humans tooth ankylosis is pathological, whereby a fusion between alveolar bone and the cementum of a tooth occurs.

Hard tissue, refers to "normal" calcified tissue, is the tissue which is mineralized and has a firm intercellular matrix. The hard tissues of humans are bone, tooth enamel, dentin, and cementum. The term is in contrast to soft tissue.

<span class="mw-page-title-main">Linear enamel hypoplasia</span>

Linear enamel hypoplasia is a failure of the tooth enamel to develop correctly during growth, leaving bands of reduced enamel on a tooth surface. It is the most common type of enamel hypoplasia reported in clinical and archaeological samples, with other types including plane-form enamel hypoplasia and pitting enamel hypoplasia.

<span class="mw-page-title-main">Dinosaur tooth</span> Subject of dental study in paleontology

Dinosaur teeth have been studied since 1822 when Mary Ann Mantell (1795-1869) and her husband Dr Gideon Algernon Mantell (1790-1852) discovered an Iguanodon tooth in Sussex in England. Unlike mammal teeth, individual dinosaur teeth are generally not considered by paleontologists to be diagnostic to the genus or species level for unknown taxa, due morphological convergence and variability between teeth. and many historically named tooth taxa like Paronychodon and Richardoestesia are today considered nomina dubia, and are used as form taxa to refer to isolated teeth from other localities displaced considerably in time and space from the type specimens. However, it is possible to refer isolated teeth to known taxa provided that the tooth morphology is known and the teeth originate from a similar time and place.

Mortuary archaeology is the study of human remains in their archaeological context. This is a known sub-field of bioarchaeology, which is a field that focuses on gathering important information based on the skeleton of an individual. Bioarchaeology stems from the practice of human osteology which is the anatomical study of skeletal remains. Mortuary archaeology, as well as the overarching field it resides in, aims to generate an understanding of disease, migration, health, nutrition, gender, status, and kinship among past populations. Ultimately, these topics help to produce a picture of the daily lives of past individuals. Mortuary archaeologists draw upon the humanities, as well as social and hard sciences to have a full understanding of the individual.

<span class="mw-page-title-main">Near Eastern bioarchaeology</span> Archaeological sub-discipline

Near Eastern bioarchaeology covers the study of human skeletal remains from archaeological sites in Cyprus, Egypt, Levantine coast, Jordan, Turkey, Iran, Saudi Arabia, Qatar, Kuwait, Bahrain, United Arab Emirates, Oman, and Yemen.

The analysis of dental remains is a valuable tool to archaeologists. Teeth are hard, highly mineralised and chemically stable, so therefore preserve well and are one of the most commonly found animals remains. Analysis of these remains also yields a wealth of information. It can not only be used to determine the sex and age of the individual whose mandibular or dental remains have been found, but can also shed light on their diet, pathology, and even their geographic origins through isotope analysis.

References

  1. "Matson's Laboratory - A wildlife lab offering cementum aging and tetracycline biomarker screening".
  2. Rendu W.; Armand D.; Pubert E.; Soressi M. (2010). "Approche taphonomique en Cémentochronologie : réexamen du niveau 4 du Pech-de-l'Azé I (Carsac, Dordogne, France)". Paléo.
  3. 1 2 Colard, T.; Bertrand, B.; Naji, S.; Delannoy, Y.; Bécart, A. (2015). "Toward the adoption of cementochronology in forensic context". International Journal of Legal Medicine. 132 (4): 1117–1124. doi:10.1007/s00414-015-1172-8. PMID   25773917. S2CID   24517675.
  4. Naji S; Colard T; Blondiaux J; Bertrand B; D’Incau E; Bocquet-Appel J-P. (2014). "Cementochronology, to cut or not to cut?". International Journal of Paleopathology. 15: 113–119. doi:10.1016/j.ijpp.2014.05.003. PMID   29539545.
  5. Grue H, Jensen B (1979). "Review of the formation of incremental lines in tooth cementum of terrestrial mammals". Danish Rev Game Biol.
  6. Mina M, Klevezal GA. Autobiographies of animals. Znanie. En. 1970
  7. Stott, G.G.; Sis, R.F.; Levy, B.M. (1982). "Cemental annulation as an age criterion in forensic dentistry". Journal of Dental Research. 61 (6): 814–817. doi:10.1177/00220345820610063401. PMID   6953121. S2CID   40681767.
  8. Dental cementum in anthropology. Stephan Naji, William Rendu, Lionel Gourichon. Cambridge, United Kingdom. 2022. ISBN   978-1-108-56950-7. OCLC   1256591198.{{cite book}}: CS1 maint: location missing publisher (link) CS1 maint: others (link)
  9. Bertrand B, Cunha E, Bécart A, Gosset D, Hédouin V (2019). "Age at death estimation by cementochronology: Too precise to be true or too precise to be accurate?". Am J Phys Anthropol. 169 (3): 464–481. doi:10.1002/ajpa.23849. PMID   31049939. S2CID   143434374.{{cite journal}}: CS1 maint: multiple names: authors list (link)