FlowJo

Last updated
FlowJo
Developer(s) FlowJo LLC
Initial release1996
Stable release
v10.10 / 16 November 2023;20 months ago (2023-11-16)
License Proprietary
Website www.flowjo.com/solutions/flowjo

FlowJo is a software package for analyzing flow cytometry data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard [1] format with an .fcs file extension. FlowJo will import and analyze cytometry data regardless of which flow cytometer is used to collect the data.

Contents

Operation

In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics). [2] Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations.

Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came. When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group. Thus, you can apply a gate to a sample, copy it to the group, and that gate will be automatically placed on all samples in the group.

FlowJo provides tools for the creation of histogram and other plot overlays, cell cycle analysis, calcium flux analysis, proliferation analysis, quantification, cluster identification, and backgating display.

Development

FlowJo became a commercial product in 1996. In 2002, Tree Star released a Windows version. FlowJo was acquired by Becton Dickinson in 2017. [3] [4]

References

  1. Seamer, L.C.; Bagwell, C.B.; Barden, L.; Redelman, D.; Salzman, G.C.; Wood, J.C.S.; Murphy, R.F. (1997). "Proposed new data file standard for flow cytometry, version FCS 3.0". Cytometry. 28 (2): 118–122. doi: 10.1002/(SICI)1097-0320(19970601)28:2<118::AID-CYTO3>3.0.CO;2-B . ISSN   0196-4763. PMID   9181300.
  2. Moran, Barry (12 May 2015). "FlowJo Software: how's your FlowJo mojo?". Bitesize Bio. Retrieved 31 July 2025.
  3. "About Us FlowJo, LLC". flowjo.com. Retrieved 31 July 2025.
  4. "Recent Mergers and Acquisitions". www.bd.com. Retrieved 31 July 2025.