Ham's tissue culture medium

Last updated

Ham's tissue culture medium is a growth medium for mammalian cells. [1]

Contents

Contents

It contains in amounts dissolved in 1 liter of triple distilled water:

L-Arginine 211 mg
Biotin 0.024 mg
L-Histidine 21 mg
Calcium pantothenate 0.7 mg
L-Lysine 29.3 mg
Choline chloride 0.69 mg
L-Methionine 4.48 mg
i-inositol 0.54 mg
L-Phenylalanine 4.96 mg
Niacinamide 0.6 mg
L-Tryptophan 0.6 mg
Pyridoxine hydrochloride 0.2 mg
L-Tyrosine 1.81 mg
Riboflavin 0.37 mg
L-Alanine 8.91 mg
Thymidine 0.7 mg
Glycine 7.51 mg
Cyanocobalamin 1.3 mg
L-Serine 10.5 mg
Sodium pyruvate 110 mg
L-Threonine 3.57 mg
Lipoic acid 0.2 mg
L-Aspartic acid 13.3 mg
CaCl2 44 mg
L-Glutamic acid 14.7 mg
MgSO4·7H2O 153 mg
L-Asparagine 15 mg
Glucose 1.1 g
L-Glutamine 146.2 mg
NaCl 7.4 g
L-Isoleucine 2.6 mg
KCl 285 mg
L-Leucine 13.1 mg
Na2HPO4 290 mg
L-Proline 11.5 mg
KH2PO4 83 mg
L-Valine 3.5 mg
Phenol red 1.2 mg
L-Cysteine 31.5 mg
FeSO4 0.83 mg
Thiamine hydrochloride 1 mg
CuSO4·5H2O 0.0025 mg
Hypoxanthine 4 mg
ZnSO4·7H2O 0.028 mg
Folic acid 1.3 mg
NaHCO3 1.2 g

See also

Related Research Articles

The term chloride refers either to a chloride ion, which is a negatively charged chlorine atom, or a non-charged chlorine atom covalently bonded to the rest of the molecule by a single bond. Many inorganic chlorides are salts. Many organic compounds are chlorides. The pronunciation of the word "chloride" is.

<span class="mw-page-title-main">Magnesium sulfate</span> Chemical compound with formula MgSO4

Magnesium sulfate or magnesium sulphate (in English-speaking countries other than the US) is a chemical compound, a salt with the formula MgSO4, consisting of magnesium cations Mg2+ (20.19% by mass) and sulfate anions SO2−4. It is a white crystalline solid, soluble in water but not in ethanol.

<span class="mw-page-title-main">Zinc sulfate</span> Chemical compound

Zinc sulfate describes a family of inorganic compounds with the formula ZnSO4(H2O)x. All are colorless solids. The most common form includes water of crystallization as the heptahydrate, with the formula ZnSO4·7H2O. It was historically known as "white vitriol". Zinc sulfate and its hydrates are colourless solids.

<span class="mw-page-title-main">Sodium sulfate</span> Chemical compound with formula Na2SO4

Sodium sulfate (also known as sodium sulphate or sulfate of soda) is the inorganic compound with formula Na2SO4 as well as several related hydrates. All forms are white solids that are highly soluble in water. With an annual production of 6 million tonnes, the decahydrate is a major commodity chemical product. It is mainly used as a filler in the manufacture of powdered home laundry detergents and in the Kraft process of paper pulping for making highly alkaline sulfides.

Minimal Essential Medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. It is based on 6 salts and glucose described in Earle's salts in 1934: (calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium phosphate and sodium bicarbonate), supplemented with 13 essential amino acids, and 8 vitamins: thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pantothenic acid (vitamin B5), pyrodoxine (vitamin B6), folic acid (vitamin B9), choline, and myo-inositol (originally known as vitamin B8).

RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center, from where it derives its name. A modification of McCoy′s 5A medium, it was originally formulated to support lymphoblastoid cells in suspension cultures, but can also support a wide variety of adherent cells.

<span class="mw-page-title-main">Growth medium</span> Solid, liquid or gel used to grow microorganisms or cells

A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss Physcomitrella patens. Different types of media are used for growing different types of cells.

<span class="mw-page-title-main">Murashige and Skoog medium</span> Growth medium used in plant cell culture

Murashige and Skoog medium is a plant growth medium used in the laboratories for cultivation of plant cell culture. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/L sucrose. Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in the laboratory, but according to the latest scientific findings, MS medium is not suitable as a nutrient solution for deep water culture or hydroponics.

<span class="mw-page-title-main">Plasmid preparation</span>

A plasmid preparation is a method of DNA extraction and purification for plasmid DNA, it is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. Many methods have been developed to purify plasmid DNA from bacteria. During the purification procedure, the plasmid DNA is often separated from contaminating proteins and genomic DNA.

Indium(III) sulfate (In2(SO4)3) is a sulfate salt of the metal indium. It is a sesquisulfate, meaning that the sulfate group occurs 11/2 times as much as the metal. It may be formed by the reaction of indium, its oxide, or its carbonate with sulfuric acid. An excess of strong acid is required, otherwise insoluble basic salts are formed. As a solid indium sulfate can be anhydrous, or take the form of a pentahydrate with five water molecules or a nonahydrate with nine molecules of water. Indium sulfate is used in the production of indium or indium containing substances. Indium sulfate also can be found in basic salts, acidic salts or double salts including indium alum.

Hanks' salts is a collective group of salts rich in bicarbonate ions, formulated in 1940 by the microbiologist John H. Hanks. Typically, they are used as a buffer system in cell culture media and aid in maintaining the optimum physiological pH for cellular growth. Due to their poorly reactive nature and small concentration in solution, Hanks' salts are mainly used in media that are exposed to atmospheric conditions as opposed to CO2 incubation. Performing the latter drastically exceeds the buffer capacity of Hanks' salts and may result in cell death.

Super Optimal Broth is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water soluble vitamins and glucose in a low-salt formulation. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB medium. Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids. SOC medium can also be used to regenerate Klebsiella oxytoca strains for the improved transformation efficiency.

<span class="mw-page-title-main">Cobalt(II) sulfate</span> Inorganic compound

Cobalt(II) sulfate is any of the inorganic compounds with the formula CoSO4(H2O)x. Usually cobalt sulfate refers to the hexa- or heptahydrates CoSO4.6H2O or CoSO4.7H2O, respectively. The heptahydrate is a red solid that is soluble in water and methanol. Since cobalt(II) has an odd number of electrons, its salts are paramagnetic.

<span class="mw-page-title-main">Tabtoxin</span> Chemical compound

Tabtoxin, also known as wildfire toxin, is a simple monobactam phytotoxin produced by Pseudomonas syringae. It is the precursor to the antibiotic tabtoxinine β-lactam (TBL). It is produced by:

The Hoagland solution is a hydroponic nutrient solution that was newly developed by Hoagland and Snyder in 1933, modified by Hoagland and Arnon in 1938, and revised by Arnon in 1950. It is one of the most popular artificial solution compositions for growing plants, in the scientific world at least, with more than 19,000 citations listed by Google Scholar. The Hoagland solution provides all essential elements for plant nutrition and is appropriate for supporting normal growth of a large variety of plant species.

This list gives an overview of the classification of non-silicate minerals and includes mostly International Mineralogical Association (IMA) recognized minerals and its groupings. This list complements the List of minerals recognized by the International Mineralogical Association series of articles and List of minerals. Rocks, ores, mineral mixtures, not IMA approved minerals, not named minerals are mostly excluded. Mostly major groups only, or groupings used by New Dana Classification and Mindat.

Persephonella marina is a Gram-negative, rod shaped bacteria that is a member of the Aquificota phylum. Stemming from Greek, the name Persephonella is based upon the mythological goddess Persephone. Marina stems from a Latin origin, meaning "belonging to the sea". It is a thermophile with an obligate chemolithoautotrophic metabolism. Growth of P. marina can occur in pairs or individually, but is rarely seen aggregating in large groups. The organism resides on sulfidic chimneys in the deep ocean and has never been documented as a pathogen.

<span class="mw-page-title-main">Leonite</span> Hydrated double sulfate of magnesium and potassium

Leonite is a hydrated double sulfate of magnesium and potassium. It has the formula K2SO4·MgSO4·4H2O. The mineral was named after Leo Strippelmann, who was director of the salt works at Westeregeln in Germany. The mineral is part of the blodite group of hydrated double sulfate minerals.

Nickel is one of the metals that can form Tutton's salts. The singly charged ion can be any of the full range of potassium, rubidium, cesium, ammonium (), or thallium. As a mineral the ammonium nickel salt, (NH4)2Ni(SO4)2 · 6 H2O, can be called nickelboussingaultite. With sodium, the double sulfate is nickelblödite Na2Ni(SO4)2 · 4 H2O from the blödite family. Nickel can be substituted by other divalent metals of similar sized to make mixtures that crystallise in the same form.

BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi. It was first described by A.J.P. Oort in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981. The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.

References

  1. Shenoy, Manjula (2007). Animal Biotechnology. Firewall Media. pp. 19–20. ISBN   978-81-318-0108-6.

Further reading