Ham's tissue culture medium

Ham's tissue culture medium is a growth medium for mammalian cells. ^[1]

Contents

It contains in amounts dissolved in 1 liter of triple distilled water:

L-Arginine 211 mg

Biotin 0.024 mg

L-Histidine 21 mg

Calcium pantothenate 0.7 mg

L-Lysine 29.3 mg

Choline chloride 0.69 mg

L-Methionine 4.48 mg

i-inositol 0.54 mg

L-Phenylalanine 4.96 mg

Niacinamide 0.6 mg

L-Tryptophan 0.6 mg

Pyridoxine hydrochloride 0.2 mg

L-Tyrosine 1.81 mg

Riboflavin 0.37 mg

L-Alanine 8.91 mg

Thymidine 0.7 mg

Glycine 7.51 mg

Cyanocobalamin 1.3 mg

L-Serine 10.5 mg

Sodium pyruvate 110 mg

L-Threonine 3.57 mg

Lipoic acid 0.2 mg

L-Aspartic acid 13.3 mg

CaCl₂ 44 mg

L-Glutamic acid 14.7 mg

MgSO₄·7H₂O 153 mg

L-Asparagine 15 mg

Glucose 1.1 g

L-Glutamine 146.2 mg

NaCl 7.4 g

L-Isoleucine 2.6 mg

KCl 285 mg

L-Leucine 13.1 mg

Na₂HPO₄ 290 mg

L-Proline 11.5 mg

KH₂PO₄ 83 mg

L-Valine 3.5 mg

Phenol red 1.2 mg

L-Cysteine 31.5 mg

FeSO₄ 0.83 mg

Thiamine hydrochloride 1 mg

CuSO₄·5H₂O 0.0025 mg

Hypoxanthine 4 mg

ZnSO₄·7H₂O 0.028 mg

Folic acid 1.3 mg

NaHCO₃ 1.2 g

See also

• Biotechnology
• Brewing
• Cell biology
• DMEM Dulbecco's Modified Eagle's Medium
• Eagle's minimal essential medium (a minimum ingredient cell culture medium)
• Fermentation (food)
• GMEM (Glasgow's Minimal Essential Medium)
• Murashige and Skoog medium (Plant cell culture medium)
• RPMI (Roswell Park Memorial Institute medium), for lymph cells

References

1. Shenoy, Manjula (2007). Animal Biotechnology. Firewall Media. pp. 19–20. ISBN   978-81-318-0108-6.

Further reading

• The Nutrient Requirements of Cells