Homeotic protein bicoid

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Homeotic protein bicoid
Identifiers
Organism Drosophila melanogaster
Symbolbcd
UniProt P09081
Search for
Structures Swiss-model
Domains InterPro
(Top) Nuclear Bicoid protein gradient in a fixed transgenic Drosophila embryo carrying a Bicoid-GFP fusion gene. Image courtesy of Julien O. Dubuis and Thomas Gregor. (Bottom) Bicoid-GFP protein (green) and FISH-labeled bicoid mRNA (red) in the anterior tip of a fixed transgenic Drosophila embryo. Both embryos are oriented with the anterior pole at left. Image courtesy of Shawn C. Little and Thomas Gregor (see Little et al. for methods ). Fluorescent labeling of Bicoid GFP and mRNA.pdf
(Top) Nuclear Bicoid protein gradient in a fixed transgenic Drosophila embryo carrying a Bicoid–GFP fusion gene. Image courtesy of Julien O. Dubuis and Thomas Gregor. (Bottom) Bicoid–GFP protein (green) and FISH-labeled bicoid mRNA (red) in the anterior tip of a fixed transgenic Drosophila embryo. Both embryos are oriented with the anterior pole at left. Image courtesy of Shawn C. Little and Thomas Gregor (see Little et al. for methods ).

Homeotic protein bicoid is encoded by the bcd maternal effect gene in Drosophilia. Homeotic protein bicoid concentration gradient patterns the anterior-posterior (A-P) axis during Drosophila embryogenesis. Bicoid was the first protein demonstrated to act as a morphogen. [2] Although bicoid is important for the development of Drosophila and other higher dipterans, [3] it is absent from most other insects, where its role is accomplished by other genes. [4] [5]

Contents

Role in axial patterning

Bicoid mRNA is actively localized to the anterior of the fruit fly egg during oogenesis [6] along microtubules [7] by the motor protein dynein, [8] and retained there through association with cortical actin. [9] Translation of bicoid is regulated by its 3′ UTR and begins after egg deposition. Diffusion and convection within the syncytium produce an exponential gradient of Bicoid protein [2] [10] within roughly one hour, after which Bicoid nuclear concentrations remain approximately constant through cellularization. [11] An alternative model proposes the formation of a bicoid mRNA gradient in the embryo along cortical microtubules which then serves as template for translation of the Bicoid protein to form the Bicoid protein gradient. [12] [13] [14] Bicoid protein represses the translation of caudal mRNA and enhances the transcription of anterior gap genes including hunchback, orthodenticle, and buttonhead.

Structure and function

PyMOL rendering of Bicoid homeodomain bound to its consensus site PyMOL rendering of Bicoid homeodomain bound to its consensus site.png
PyMOL rendering of Bicoid homeodomain bound to its consensus site

Bicoid is one of the few proteins which uses its homeodomain to bind both DNA and RNA targets to regulate their transcription and translation, respectively. The nucleic acid-binding homeodomain of Bicoid has been solved by NMR. [15] Bicoid contains an arginine-rich motif (part of the helix shown axially in this image) that is similar to the one found in the HIV protein REV and is essential for its nucleic acid binding. [16]

Bicoid mutant produces no head Bicoid mutant.jpeg
Bicoid mutant produces no head

Bicoid protein gradient formation is one of the earliest steps in fruit fly embryo A-P patterning. The proper spatial expression of downstream genes relies on the robustness of this gradient to common variations between embryos, including in the number of maternally-deposited bicoid mRNAs and in egg size. Comparative phylogenetic [17] and experimental evolution [18] studies suggest an inherent mechanism for robust generation of a scaled Bicoid protein gradient. Mechanisms that have been proposed to effect this scaling include non-linear degradation of Bicoid, [19] nuclear retention as a size-dependent regulator of Bicoid protein's effective diffusion coefficient, [10] [20] and scaling of cytoplasmic streaming. [10]

See also

Related Research Articles

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<span class="mw-page-title-main">Bicoid 3′-UTR regulatory element</span>

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References

  1. ↑ Little SC, Tkačik G, Kneeland TB, Wieschaus EF, Gregor T (March 2011). "The formation of the Bicoid morphogen gradient requires protein movement from anteriorly localized mRNA". PLOS Biology. 9 (3): e1000596. doi: 10.1371/journal.pbio.1000596 . PMC   3046954 . PMID   21390295.
  2. 1 2 Driever W, Nüsslein-Volhard C (July 1988). "The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner". Cell. 54 (1): 95–104. doi:10.1016/0092-8674(88)90183-3. PMID   3383245. S2CID   18830552.
  3. ↑ Gregor T, McGregor AP, Wieschaus EF (April 2008). "Shape and function of the Bicoid morphogen gradient in dipteran species with different sized embryos". Developmental Biology. 316 (2): 350–358. doi:10.1016/j.ydbio.2008.01.039. PMC   2441567 . PMID   18328473.
  4. ↑ Chouard T (November 2008). "Darwin 200: Beneath the surface". Nature. 456 (7220): 300–303. doi: 10.1038/456300a . PMID   19020592.
  5. ↑ Schröder R (April 2003). "The genes orthodenticle and hunchback substitute for bicoid in the beetle Tribolium". Nature. 422 (6932): 621–625. Bibcode:2003Natur.422..621S. doi:10.1038/nature01536. PMID   12687002. S2CID   4406927.
  6. ↑ St Johnston D, Driever W, Berleth T, Richstein S, Nusslein-Volhard C (1989). "Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte". Development. 107: 13–19. doi:10.1242/dev.107.Supplement.13. PMID   2483989.
  7. ↑ Pokrywka NJ, Stephenson EC (September 1991). "Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis". Development. 113 (1): 55–66. doi: 10.1242/dev.113.1.55 . PMID   1684934.
  8. ↑ Weil TT, Forrest KM, Gavis ER (August 2006). "Localization of bicoid mRNA in late oocytes is maintained by continual active transport". Developmental Cell. 11 (2): 251–262. doi: 10.1016/j.devcel.2006.06.006 . PMID   16890164.
  9. ↑ Weil TT, Parton R, Davis I, Gavis ER (July 2008). "Changes in bicoid mRNA anchoring highlight conserved mechanisms during the oocyte-to-embryo transition". Current Biology. 18 (14): 1055–1061. Bibcode:2008CBio...18.1055W. doi:10.1016/j.cub.2008.06.046. PMC   2581475 . PMID   18639459.
  10. 1 2 3 Hecht I, Rappel WJ, Levine H (February 2009). "Determining the scale of the Bicoid morphogen gradient". Proceedings of the National Academy of Sciences of the United States of America. 106 (6): 1710–1715. Bibcode:2009PNAS..106.1710H. doi: 10.1073/pnas.0807655106 . PMC   2644102 . PMID   19190186.
  11. ↑ Gregor T, Wieschaus EF, McGregor AP, Bialek W, Tank DW (July 2007). "Stability and nuclear dynamics of the bicoid morphogen gradient". Cell. 130 (1): 141–152. doi:10.1016/j.cell.2007.05.026. PMC   2253672 . PMID   17632061.
  12. ↑ Frigerio G, Burri M, Bopp D, Baumgartner S, Noll M (December 1986). "Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network". Cell. 47 (5): 735–746. doi:10.1016/0092-8674(86)90516-7. PMID   2877746. S2CID   9658875.
  13. ↑ Spirov A, Fahmy K, Schneider M, Frei E, Noll M, Baumgartner S (February 2009). "Formation of the bicoid morphogen gradient: an mRNA gradient dictates the protein gradient". Development. 136 (4): 605–614. doi:10.1242/dev.031195. PMC   2685955 . PMID   19168676.
  14. ↑ Fahmy K, Akber M, Cai X, Koul A, Hayder A, Baumgartner S (2014). "αTubulin 67C and Ncd are essential for establishing a cortical microtubular network and formation of the Bicoid mRNA gradient in Drosophila". PLOS ONE. 9 (11): e112053. Bibcode:2014PLoSO...9k2053F. doi: 10.1371/journal.pone.0112053 . PMC   4229129 . PMID   25390693.
  15. ↑ Baird-Titus JM, Clark-Baldwin K, Dave V, Caperelli CA, Ma J, Rance M (March 2006). "The solution structure of the native K50 Bicoid homeodomain bound to the consensus TAATCC DNA-binding site". Journal of Molecular Biology. 356 (5): 1137–1151. doi:10.1016/j.jmb.2005.12.007. PMID   16406070.
  16. ↑ Niessing D, Driever W, Sprenger F, Taubert H, Jäckle H, Rivera-Pomar R (February 2000). "Homeodomain position 54 specifies transcriptional versus translational control by Bicoid". Molecular Cell. 5 (2): 395–401. doi:10.1016/S1097-2765(00)80434-7. hdl: 11858/00-001M-0000-0028-A069-9 . PMID   10882080.
  17. ↑ Gregor T, McGregor AP, Wieschaus EF (April 2008). "Shape and function of the Bicoid morphogen gradient in dipteran species with different sized embryos". Developmental Biology. 316 (2): 350–358. doi:10.1016/j.ydbio.2008.01.039. PMC   2441567 . PMID   18328473.
  18. ↑ Cheung D, Miles C, Kreitman M, Ma J (January 2014). "Adaptation of the length scale and amplitude of the Bicoid gradient profile to achieve robust patterning in abnormally large Drosophila melanogaster embryos". Development. 141 (1): 124–135. doi:10.1242/dev.098640. PMC   3865754 . PMID   24284208.
  19. ↑ Eldar A, Rosin D, Shilo BZ, Barkai N (October 2003). "Self-enhanced ligand degradation underlies robustness of morphogen gradients". Developmental Cell. 5 (4): 635–646. doi: 10.1016/S1534-5807(03)00292-2 . PMID   14536064.
  20. ↑ Grimm O, Wieschaus E (September 2010). "The Bicoid gradient is shaped independently of nuclei". Development. 137 (17): 2857–2862. doi:10.1242/dev.052589. PMC   2938918 . PMID   20699297.
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