Henry Stuart, Lord Darnley, was the second husband of Mary, Queen of Scots, and the father of James VI of Scotland and I of England. Through his parents, he had claims to both the Scottish and English thrones, and from his marriage in 1565 he was king consort of Scotland. Less than a year after the birth of his son, Darnley was murdered at Kirk o' Field in 1567. Many contemporary narratives describing his life and death refer to him as simply Lord Darnley, his title as heir apparent to the Earldom of Lennox.
James Hepburn, 1st Duke of Orkney and 4th Earl of Bothwell, better known simply as Lord Bothwell, was a prominent Scottish nobleman. He was known for his marriage to Mary, Queen of Scots, as her third and final husband. He was accused of the murder of Mary's second husband, Henry Stuart, Lord Darnley, a charge of which he was acquitted. His marriage to Mary was controversial and divided the country; when he fled the growing rebellion to Norway, he was arrested and lived the rest of his life imprisoned in Denmark.
Patrick Hepburn, 3rd Earl of Bothwell was the son of Adam Hepburn, Lord Hailes, who died at the Battle of Flodden the year after Patrick's birth.
George Gordon, 5th Earl of Huntly, was Lord Chancellor of Scotland and major conspirator of his time.
Robert Stewart, 1st Earl of Orkney and Lord of Zetland (Shetland) was a recognised illegitimate son of James V, King of Scotland, and his mistress Eupheme Elphinstone. Robert Stewart was half-brother to Mary, Queen of Scots and uncle to James VI and I of Scotland and England.
Mary Beaton, or Bethune as she wrote her family name, was a Scottish courtier. She is remembered in history as one of the four girls who were companions of Mary, Queen of Scots from childhood, known as The Queen's Maries or The Four Maries, and has also entered folklore through the traditional ballad of Marie Hamilton.
Morham, East Lothian, sometimes spelt Moram, Morum, or Morhame in old records, is the smallest (agricultural) parish in Scotland, sandwiched between five other parishes: Haddington, Garvald, Yester, Whittingehame, and Prestonkirk, in the undulating lower reaches of the Lammermuir Hills.
Archibald Douglas, Parson of Douglas, was also Parson of Glasgow, a Senator of the College of Justice, Ambassador to Queen Elizabeth I of England, and a notorious intriguer.
Sir Robert Lauder of Popill was a Scottish landowner and an adherent of Mary, Queen of Scots.
The murder of Henry Stuart, Lord Darnley, second husband of Mary, Queen of Scots, took place on 10 February 1567 in Edinburgh, Scotland. Darnley's lodgings were destroyed by gunpowder; his body and that of his servant were found nearby, apparently having been strangled rather than killed in the explosion. Suspicion was placed upon Queen Mary and the Earl of Bothwell, whom Mary went on to marry three months after Darnley's murder. Bothwell was indicted for treason and acquitted, but six of his servants and acquaintances were subsequently arrested, tried, and executed for the crime.
Francis Stewart, 5th Earl of Bothwell, was Commendator of Kelso Abbey and Coldingham Priory, a Privy Counsellor and Lord High Admiral of Scotland. He was a notorious conspirator who led several uprisings against his first cousin, King James VI, all of which ultimately failed, and he died in poverty in Italy after being banished from Scotland. Francis's maternal uncle, the 4th Earl of Bothwell, was the chief suspect in the murder of James VI's father, Lord Darnley.
Elizabeth Keith, Countess of Huntly, was a Scottish noblewoman and the wife of George Gordon, 4th Earl of Huntly, Scotland's leading Catholic magnate during the reign of Mary, Queen of Scots. In 1562, Elizabeth encouraged her husband to raise forces against Queen Mary which led to his being outlawed, and after his death, his titles forfeited to the Crown. Elizabeth's son Sir John Gordon was executed for having taken part in his father's rebellion.
Jean Gordon, Countess of Bothwell was a wealthy Scottish noblewoman and the second wife of James Hepburn, 4th Earl of Bothwell. He became, after his divorce from Lady Jean, the third husband of Mary, Queen of Scots. Lady Jean herself had a total of three husbands. Upon her second marriage, she became the Countess of Sutherland.
Janet Beaton, Lady of Branxholme and Buccleugh (1519–1569) was an aristocratic Scottish woman and a mistress of James Hepburn, Earl of Bothwell. She had a total of five husbands. One of her nieces was Mary Beaton, one of the four ladies-in-waiting of Mary, Queen of Scots, known in history as the four Marys. In her lifetime, she was accused of having been a witch. Janet was immortalised as Sir Walter Scott's Wizard Lady of Branxholm in his celebrated narrative poem "Lay of the Last Minstrel".
Anne Hamilton, Countess of Huntly, was a Scottish noblewoman and a member of the powerful Hamilton family which had a strong claim to the Scottish crown. Her father James Hamilton, Duke of Châtellerault, 2nd Earl of Arran was heir presumptive to the throne of Scotland after Mary, Queen of Scots prior to the birth of the latter's son Prince James in 1566. Anne was the wife of George Gordon, 5th Earl of Huntly, Lord Chancellor of Scotland and a chief conspirator during the reign of Queen Mary.
The Ainslie Tavern Bond was a document signed on about 20 April 1567 by a number of Scottish bishops and nobles. The bond approved the Earl of Bothwell's acquittal on 12 April of implication in the murder of Lord Darnley, recommended him as an appropriate husband for Mary, Queen of Scots, and pledged to assist in defending such a marriage.
Laurence Oliphant, 4th Lord Oliphant (1529–1593) was a Scottish nobleman.
George Sinclair was a Scottish nobleman, the 4th Earl of Caithness and chief of the Clan Sinclair, a Scottish clan of the Scottish Highlands.
Margaret Beaton, Lady Reres was a Scottish courtier and companion of Mary of Guise and Mary, Queen of Scots. She was blamed by the enemies of Mary, Queen of Scots, for her involvement in alleged immorality at court.
John Stewart, Commendator of Coldingham (1531–1563) was a Scottish landowner.
