James Stewart, 1st Earl of Moray was a member of the House of Stewart as the illegitimate son of King James V of Scotland. At times a supporter of his half-sister Mary, Queen of Scots, he was the regent of Scotland for his half-nephew, the infant King James VI, from 1567 until his assassination in 1570. He was the first head of government to be assassinated with a firearm.
Francis Hay, 9th Earl of Erroll was a Scottish nobleman. A convert to Catholicism, he openly conspired with the king of Spain to try to unseat the Protestant Queen Elizabeth.
James Hamilton, 3rd Earl of Arran (1537–1609) was a Scottish nobleman and soldier who opposed the French-dominated regency during the Scottish Reformation. He was the eldest son of James Hamilton, Duke of Châtellerault, sometime regent of Scotland. He was of royal descent, and at times was third or fourth in succession to the Scottish crown; several royal marriages were proposed for him, but he eventually never married. He went to France with Mary, Queen of Scots, where he commanded the Scots Guards. After returning to Scotland, he became a leader of the Protestant party against Mary and her French supporters. However, he went insane in 1562 and was confined for the rest of his life.
William Douglas, 6th Earl of Morton was the son of Robert Douglas of Lochleven and Margaret Erskine, a former mistress of James V of Scotland.
John Stewart, 1st Earl of Traquair was a Scottish statesman who was created Baron Stewart of Traquair in 1628 and Earl of Traquair in 1633.
George Seton, 3rd Earl of Winton was a notable Royalist and Cavalier, the second son of Robert Seton, 1st Earl of Winton and 6th Lord Seton, by his spouse Margaret, daughter of Hugh Montgomerie, 3rd Earl of Eglinton.
Andrew Stewart, 2nd Lord Ochiltree fought for the Scottish Reformation. His daughter married John Knox and he played a part in the defeat of Mary, Queen of Scots at the battle of Langside.
John Fleming, 5th Lord Fleming, was a Scottish nobleman and a supporter of Mary, Queen of Scots.
William Livingstone, 6th Lord Livingston,, was a Scottish lord of Parliament.
Margaret Stewart, Mistress of Ochiltree was a courtier in the household of Anne of Denmark in Scotland and looked after her children Prince Henry, Princess Elizabeth, and Charles I of England
James Stewart, 1st Lord Doune (1529-1590) was a Scottish landowner.
Sir John Gordon of Lochinvar and Kenmure, was a Scottish courtier, landowner, and supporter of Mary, Queen of Scots.
Margaret Fleming, Countess of Atholl (1536-1586) was a Scottish courtier and landowner rumoured to be involved in the occult. She served as lady-in-waiting to Mary, Queen of Scots.
William Hay, 6th Lord Hay of Yester was a Scottish nobleman and courtier.
John Stewart, Commendator of Coldingham (1531–1563) was a Scottish landowner.
John Erskine, 3rd Earl of Mar was a Scottish landowner.
Anna Hay, Countess of Winton (1592-1628) was a Scottish courtier.
James Stewart, Commendator of Kelso and Melrose was a member of the Scottish royal family.
William Stewart of Caverston and Traquair, was a Scottish landowner and Captain of Dumbarton Castle.
Arthur Erskine of Blackgrange was a Scottish courtier.
