The following people played for the Panthers for at least one game in the 1926 AFL regular season, the only one of the team's (and the league's) existence: [1]
| Name | Position | College |
|---|---|---|
| Norty "Mope" Behm | End1 | Iowa State |
| Cookie Cunningham | End | Ohio State |
| Doc Elliott | Fullback | Lafayette |
| Myles Evans | Tackle | Ohio Wesleyan |
| Billy Gribben | Tailback | Case Western Reserve |
| Eddie "Red" Kregenow | End | Akron |
| Al Michaels | Tailback | Heidelberg, Ohio State |
| Al Nesser 2 | Guard3 | none |
| Dave Noble | Back4 | Nebraska |
| John Otterbacher | Guard | Ohio State |
| Guy Roberts | Back4 | Dayton |
| Red Roberts | Tackle | Centre |
| Jack Sack | Guard | Pittsburgh |
| Bob Spiers | Tackle5 | Ohio State |
| Al Thornburg | Center | Iowa State |
| Ralph Vince | Guard | Washington & Jefferson |
| Leo Virant | Guard | Iowa State |
| Red Weaver | Center | Centre |
| Jay Winters | Blocking back6 | Ohio Wesleyan |
| Dick Wolf | Blocking back | Miami (Ohio) |
1 Also played fullback
2 Started 1926 season as coach of Akron Indians, then left to play for Panthers
3 Also played tackle
4 Played wingback and American football
5 Also played guard
6 Position currently known as quarterback