Orthochromasia

Last updated

In chemistry, orthochromasia is the property of a dye or stain to not change color on binding to a target, as opposed to metachromatic stains, which change color. The word is derived from the Greek orthos (correct, upright), and chromatic (color). Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red).

Contents

In spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming either shorter or longer.

In photography, an orthochromatic light spectrum is one devoid of red light.

In biology, orthochromatic refers to the greyish staining because of acidophilic and basophilic mixture in the cell.

Orthochromatic photography

This WW II British Hawker Tornado prototype's 'Type A.1' RAF roundel's outermost, chrome yellow ring renders as dark gray, due to orthochromasia. 15 Hawker Tornado HG641 (15833937541).jpg
This WW II British Hawker Tornado prototype's 'Type A.1' RAF roundel's outermost, chrome yellow ring renders as dark gray, due to orthochromasia.
The Union Jack on orthochromatic emulsion at the South Magnetic Pole in 1909. Northernparty.png
The Union Jack on orthochromatic emulsion at the South Magnetic Pole in 1909.

Orthochromatic photography refers to a photographic emulsion that is sensitive to only blue and green light, and thus can be processed with a red safelight. The increased blue sensitivity causes blue objects to appear lighter, and red ones darker. A cyan lens filter (which removes red light) can be used with standard panchromatic film to produce a similar effect. [1]

Orthochromatic films were first produced by Hermann Wilhelm Vogel in 1873 by adding small amounts of certain aniline-based dyes to photographic emulsions, which until that time had been sensitive to blue light only. This work was extended by others including Josef Maria Eder, who introduced the use of the red dye erythrosine in 1884. [2]

Comparison between orthochromatic and panchromatic film. Comparison between orthochromatic and panchromatic film.jpg
Comparison between orthochromatic and panchromatic film.

See also

Related Research Articles

Film stock Medium used for recording motion pictures

Film stock is an analog medium that is used for recording motion pictures or animation. It is recorded on by a movie camera, developed, edited, and projected onto a screen using a movie projector. It is a strip or sheet of transparent plastic film base coated on one side with a gelatin emulsion containing microscopically small light-sensitive silver halide crystals. The sizes and other characteristics of the crystals determine the sensitivity, contrast and resolution of the film. The emulsion will gradually darken if left exposed to light, but the process is too slow and incomplete to be of any practical use. Instead, a very short exposure to the image formed by a camera lens is used to produce only a very slight chemical change, proportional to the amount of light absorbed by each crystal. This creates an invisible latent image in the emulsion, which can be chemically developed into a visible photograph. In addition to visible light, all films are sensitive to X-rays and high-energy particles. Most are at least slightly sensitive to invisible ultraviolet (UV) light. Some special-purpose films are sensitive into the infrared (IR) region of the spectrum.

Photographic paper Light-sensitive paper used to make photographic prints

Photographic paper is a paper coated with a light-sensitive chemical formula, like photographic film, used for making photographic prints. When photographic paper is exposed to light, it captures a latent image that is then developed to form a visible image; with most papers the image density from exposure can be sufficient to not require further development, aside from fixing and clearing, though latent exposure is also usually present. The light-sensitive layer of the paper is called the emulsion. The most common chemistry was based on Silver halide but other alternatives have also been used.

Monochrome Composed of one color

A monochromic image is composed of one color. The term monochrome comes from Ancient Greek μονόχρωμος (monochromos) 'having one color'.

Color photography Photography that uses media capable of representing colors

Color photography is photography that uses media capable of capturing and reproducing colors. By contrast, black-and-white (monochrome) photography records only a single channel of luminance (brightness) and uses media capable only of showing shades of gray.

Photographic printing is the process of producing a final image on paper for viewing, using chemically sensitized paper. The paper is exposed to a photographic negative, a positive transparency , or a digital image file projected using an enlarger or digital exposure unit such as a LightJet or Minilab printer. Alternatively, the negative or transparency may be placed atop the paper and directly exposed, creating a contact print. Digital photographs are commonly printed on plain paper, for example by a color printer, but this is not considered "photographic printing".

C-41 is a chromogenic color print film developing process introduced by Kodak in 1972, superseding the C-22 process. C-41, also known as CN-16 by Fuji, CNK-4 by Konica, and AP-70 by AGFA, is the most popular film process in use, with most photofinishing labs devoting at least one machine to this development process.

Safelight Light source suitable for use in a darkroom

A safelight is a light source suitable for use in a photographic darkroom. It provides illumination only from parts of the visible spectrum to which the photographic material in use is nearly, or completely insensitive.

Infrared photography

In infrared photography, the film or image sensor used is sensitive to infrared light. The part of the spectrum used is referred to as near-infrared to distinguish it from far-infrared, which is the domain of thermal imaging. Wavelengths used for photography range from about 700 nm to about 900 nm. Film is usually sensitive to visible light too, so an infrared-passing filter is used; this lets infrared (IR) light pass through to the camera, but blocks all or most of the visible light spectrum.

Panchromatic emulsion is a type of black-and-white photographic emulsion that is sensitive to all wavelengths of visible light.

Actinism is the property of solar radiation that leads to the production of photochemical and photobiological effects. Actinism is derived from the Ancient Greek ἀκτίς, ἀκτῖνος. The word actinism is found, for example, in the terminology of imaging technology, medicine, and chemistry, and the concept of actinism is applied, for example, in chemical photography and X-ray imaging.

Film tinting is the process of adding color to black-and-white film, usually by means of soaking the film in dye and staining the film emulsion. The effect is that all of the light shining through is filtered, so that what would be white light becomes light of some color.

Chromogenic photography is photography that works by a chromogen forming a conventional silver image and then replacing it with a dye image. Most films and papers used for color photography today are chromogenic, using three layers, each providing their own subtractive color. Some chromogenic films provide black-and-white negatives, and are processed in standard color developers. In this case, the dyes are a neutral color.

A chromogenic print, also known as a C-print or C-type print, a silver halide print, or a dye coupler print, is a photographic print made from a color negative, transparency or digital image, and developed using a chromogenic process. They are composed of three layers of gelatin, each containing an emulsion of silver halide, which is used as a light-sensitive material, and a different dye coupler of subtractive color which together, when developed, form a full-color image.

In cinematography, bipacking, or a bipack, is the process of loading two reels of film into a camera, so that they both pass through the camera gate together. It was used both for in-camera effects and as an early subtractive colour process.

Color motion picture film

Color motion picture film refers both to unexposed color photographic film in a format suitable for use in a motion picture camera, and to finished motion picture film, ready for use in a projector, which bears images in color.

Photographic emulsion is a light-sensitive colloid used in film-based photography. Most commonly, in silver-gelatin photography, it consists of silver halide crystals dispersed in gelatin. The emulsion is usually coated onto a substrate of glass, films, paper, or fabric.

Dye transfer is a continuous-tone color photographic printing process. It was used to print Technicolor films, as well as to produce paper colour prints used in advertising, or large transparencies for display.

In bipack color photography for motion pictures, two strips of black-and-white 35 mm film, running through the camera emulsion to emulsion, are used to record two regions of the color spectrum, for the purpose of ultimately printing the images, in complementary colors, superimposed on one strip of film. The result is a multicolored projection print that reproduces a useful but limited range of color by the subtractive color method. Bipack processes became commercially practical in the early 1910s when Kodak introduced duplitized film print stock, which facilitated making two-color prints.

Full-spectrum photography

Full-spectrum photography is a subset of multispectral imaging, defined among photography enthusiasts as imaging with consumer cameras the full, broad spectrum of a film or camera sensor bandwidth. In practice, specialized broadband/full-spectrum film captures visible and near infrared light, commonly referred to as the "VNIR".

Photographic film Film used by film (analog) cameras

Photographic film is a strip or sheet of transparent film base coated on one side with a gelatin emulsion containing microscopically small light-sensitive silver halide crystals. The sizes and other characteristics of the crystals determine the sensitivity, contrast, and resolution of the film.

References

  1. Hulfish, David Sherrill (1970) [first published 1915]. Motion-Picture Work: The Literature of Cinema. Ayer Publishing. p. 206. ISBN   978-0405016172.
  2. Glossary: Photography: Orthochromatic. About.com. Archived from the original on 2006-09-24. Retrieved 2012-09-27.