ROSA26

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ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. [1] It was first isolated in 1991 [2] in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus. [3] The human homolog of the ROSA26 locus has been identified. [4] ROSA stands for Reverse Orientation Splice Acceptor, named after the lentivirus genetrap vector. [5]

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References

  1. "rosa26" . Retrieved 8 January 2013.
  2. Friedrich, G; Soriano, P (1991). "Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice". Genes & Development. 5 (9): 1513–23. doi: 10.1101/gad.5.9.1513 . PMID   1653172.
  3. Casola, S (2010). "Mouse models for miRNA expression: The ROSA26 locus". Methods in Molecular Biology. Methods in Molecular Biology. 667: 145–63. doi:10.1007/978-1-60761-811-9_10. ISBN   978-1-60761-810-2. PMID   20827532.
  4. Irion, Stefan; Luche, Hervé; Gadue, Paul; Fehling, Hans Joerg; Kennedy, Marion; Keller, Gordon (2007). "Identification and targeting of the ROSA26 locus in human embryonic stem cells". Nature Biotechnology. 25 (12): 1477–82. doi:10.1038/nbt1362. PMID   18037879.
  5. Friedrich, G.; Soriano, P. (September 1991). "Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice". Genes & Development. 5 (9): 1513–1523. doi: 10.1101/gad.5.9.1513 . ISSN   0890-9369. PMID   1653172.