Force spectroscopy is a set of techniques for the study of the interactions and the binding forces between individual molecules. These methods can be used to measure the mechanical properties of single polymer molecules or proteins, or individual chemical bonds. The name "force spectroscopy", although widely used in the scientific community, is somewhat misleading, because there is no true matter-radiation interaction.
Carlos José Bustamante is a Peruvian-American scientist. He is a member of the National Academy of Sciences.
Molecular biophysics is a rapidly evolving interdisciplinary area of research that combines concepts in physics, chemistry, engineering, mathematics and biology. It seeks to understand biomolecular systems and explain biological function in terms of molecular structure, structural organization, and dynamic behaviour at various levels of complexity. This discipline covers topics such as the measurement of molecular forces, molecular associations, allosteric interactions, Brownian motion, and cable theory. Additional areas of study can be found on Outline of Biophysics. The discipline has required development of specialized equipment and procedures capable of imaging and manipulating minute living structures, as well as novel experimental approaches.
Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.
Erich Sackmann is a German experimental physicist and a pioneer of biophysics in Europe.
Single-particle tracking (SPT) is the observation of the motion of individual particles within a medium. The coordinates time series, which can be either in two dimensions (x, y) or in three dimensions (x, y, z), is referred to as a trajectory. The trajectory is typically analyzed using statistical methods to extract information about the underlying dynamics of the particle. These dynamics can reveal information about the type of transport being observed (e.g., thermal or active), the medium where the particle is moving, and interactions with other particles. In the case of random motion, trajectory analysis can be used to measure the diffusion coefficient.
Microrheology is a technique used to measure the rheological properties of a medium, such as microviscosity, via the measurement of the trajectory of a flow tracer. It is a new way of doing rheology, traditionally done using a rheometer. There are two types of microrheology: passive microrheology and active microrheology. Passive microrheology uses inherent thermal energy to move the tracers, whereas active microrheology uses externally applied forces, such as from a magnetic field or an optical tweezer, to do so. Microrheology can be further differentiated into 1- and 2-particle methods.
The Alexander Hollaender Award in Biophysics is awarded by the U.S. National Academy of Sciences "for outstanding contributions in biophysics". Named in honor of Alexander Hollaender, it has been awarded every three years since 1998.
The following outline is provided as an overview of and topical guide to biophysics:
Martin Gruebele is a German-born American physical chemist and biophysicist who is currently James R. Eiszner Professor of Chemistry, Professor of Physics, Professor of Biophysics and Computational Biology at the University of Illinois Urbana-Champaign, where he is the principal investigator of the Gruebele Group.
Taekjip Ha is a South Korean-born American biophysicist who is currently a Bloomberg Distinguished Professor of Biophysics and Biomedical Engineering at Johns Hopkins University. He was previously the Gutgsell Professor of Physics, at University of Illinois at Urbana-Champaign where he was the principal investigator of Single Molecule Nanometry group. He is also a Howard Hughes Medical Institute investigator.
Cell biophysics is a sub-field of biophysics that focuses on physical principles underlying cell function. Sub-areas of current interest include statistical models of intracellular signaling dynamics, intracellular transport, cell mechanics, molecular motors, biological electricity and genetic network theory. The field has benefited greatly from recent advances in live-cell molecular imaging techniques that allow spatial and temporal measurement of macromolecules and macromolecular function. Specialized imaging methods like FRET, FRAP, photoactivation and single molecule imaging have proven useful for mapping macromolecular transport, dynamic conformational changes in proteins and macromolecular interactions. Super-resolution microscopy allows imaging of cell structures below the optical resolution of light. Combining novel experimental tools with mathematical models grounded in the physical sciences has enabled significant recent breakthroughs in the field. Multiple centers across the world are advancing the research area
Force Spectrum Microscopy (FSM) is an application of active microrheology developed to measure aggregate random forces in the cytoplasm. Large, inert flow tracers are injected into live cells and become lodged inside the cytoskeletal mesh, wherein it is oscillated by repercussions from active motor proteins. The magnitude of these random forces can be inferred from the frequency of oscillation of tracer particles. Tracking the fluctuations of tracer particles using optical microscopy can isolate the contribution of active random forces to intracellular molecular transport from that of Brownian motion.
Alireza Mashaghi is a physician-scientist and biophysicist at Leiden University. He is known for his contributions to single-molecule analysis of chaperone assisted protein folding, molecular topology and medical systems biophysics and bioengineering. He is a leading advocate for interdisciplinary research and education in medicine and pharmaceutical sciences.
Suliana Manley is an American biophysicist. Her research focuses on the development of high-resolution optical instruments, and their application in studying the organization and dynamics of proteins. She is a professor at École Polytechnique Fédérale de Lausanne and heads the Laboratory of Experimental Biophysics.
Jennifer L. Ross is an American physicist who is Professor and Chair of the Department of Physics at Syracuse University. Her research considers active biological condensed matter physics. She was elected fellow of the American Physical Society in 2018 and American Association for the Advancement of Science in 2022.
Michelle Dong Wang is a Chinese-American physicist who is the James Gilbert White Distinguished Professor of the Physical Sciences at Cornell University. She is an Investigator of the Howard Hughes Medical Institute. Her research considers biomolecular motors and single molecule optical trapping techniques. She was appointed Fellow of the American Physical Society in 2009.
Julie Biteen is a Canadian-born American chemist who is professor of chemistry and biophysics at the University of Michigan. Her research considers the development of imaging systems for biological systems. She was named the Stanford University Sessler Distinguished Alumni Lecturer in 2021.
Nynke Hester Dekker is a Dutch biophysicist who is Professor of Molecular Biophysics at the Kavli Institute of Nanoscience at Delft University of Technology. Dekker studies individual DNA and RNA molecules and how they interact with proteins in bacteria, viruses and eukaryotes. She described how virus proteins build errors into the virus RNA of viruses. In 2020, she was awarded the Spinoza Prize.
