Root sheath may refer to any of these biological structures:
- Epithelial root sheath, a proliferation of epithelial cells located at the cervical loop of the enamel organ in a developing tooth
- Root sheath (hair), the inner or epidermic coat of the hair follicle that is closely adherent to the root of the hair
- Seed root sheath, a protective layer of tissue that surrounds the radicle in monocotyledon seeds
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