Stuck fermentation

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Chardonnay fermenting. Oak chips in chardonnay.jpg
Chardonnay fermenting.

A stuck fermentation occurs in brewing beer or winemaking when the yeast become dormant before the fermentation has completed. Unlike an "arrested fermentation" where the winemaker intentionally stops fermentation (such as in the production of fortified wines), a stuck fermentation is an unintentional and unwanted occurrence that can lead to the wine being spoiled by bacteria and oxidation. There are several potential causes of a stuck fermentation; the most common are excessively high temperatures killing off the yeast, or a must deficient in the nitrogen food source needed for the yeast to thrive.[ citation needed ] Once the fermentation is stuck, it is very difficult to restart due to a chemical compound released by dying yeast cells that inhibit the future growth of yeast cells in the batch.[ citation needed ] Winemakers often take several steps to limit the possibility of a stuck fermentation occurring, such as adding nitrogen to the must in the form of diammonium phosphate or using cultured yeast with a high temperature and alcohol tolerance. These steps will each have their own subtle or dramatic effect on the resulting flavors and quality of the wine. [1]

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Possible causes

There are several potential instigators of a stuck fermentation. One of the most common found in winemaking is a nitrogen deficient must. Nitrogen is a vital nutrient in the growth and development of yeasts and is usually provided from the wine grapes themselves. Grapes grown in vineyards with soils lacking in nitrogen or grape varieties, such as Chardonnay and Riesling, which are naturally prone to have low nitrogen to sugar ratios will be at greater risk for having a stuck fermentation. Another cause rooted in the vineyard is from overripe grapes. Grapes that are overripe will have high levels of sugars that translates into higher alcohol content. Yeast are unable to reproduce in an environment with 16-18% ABV but in an environment with multiple stressors the fermentation could get stuck even before the alcohol level reaches that point. [1]

The skins included in the fermentation of red wine provide some nutrients to the yeast. Mthomebrew maceration.JPG
The skins included in the fermentation of red wine provide some nutrients to the yeast.

A byproduct of the energy created during fermentation is heat which raises the temperature of the fermenting must as the yeast work. When temperatures near 104°F (40°C), activity slows and yeast start to die. If temperatures stay high and the yeast stop reproducing then the fermentation is at grave risk of getting stuck. Even if the must is cooled back down, fermentation will be very difficult to restart due to a chemical compound released by the dying yeast that serves as an inhibitor to the growth of future yeast cells in the batch. Modern winemaking equipment includes temperature control mechanisms such as stainless steel fermenting tanks with a cooling jacket to help regulate temperatures. Another potential cause is a combination of lack of oxygen and yeast nutrients found in the lipids suspended in grape solids. In the absence of oxygen, yeast will seek out the lipids in grape solid as a source of nutrients to sustain its activity. If the wine is lacking grape solids (such as if the wine's been clarified) then the yeast are at risk of starving and causing the fermentation to get stuck. This risk is lower for red wines which are often fermented with their skins in open air containers or oak wine barrels which provide plenty of oxygen and grape solids for the yeast to feed on. [1]

Prevention techniques

There are various techniques that a winemaker can employ to minimize the chances of a stuck fermentation happening. The most common is to use a cultured yeast strain with a high alcohol and high temperature tolerance coupled by diligent control of the fermentation temperature. Another technique is to add yeast nutrients like nitrogen to the must. The ammonium salt diammonium phosphate, or yeast nutrient, is a popular inexpensive means of ensuring the must has sufficient nitrogen. Whatever technique the winemaker uses, each has the potential to subtly or drastically affect the resulting flavor and quality of the wine. [1]

Related Research Articles

Winemaking Production of wine

Winemaking or vinification is the production of wine, starting with the selection of the fruit, its fermentation into alcohol, and the bottling of the finished liquid. The history of wine-making stretches over millennia. The science of wine and winemaking is known as oenology. A winemaker may also be called a vintner. The growing of grapes is viticulture and there are many varieties of grapes.

Red wine Wine made from dark-colored grape varieties

Red wine is a type of wine made from dark-colored grape varieties. The color of the wine can range from intense violet, typical of young wines, through to brick red for mature wines and brown for older red wines. The juice from most purple grapes is greenish-white, the red color coming from anthocyan pigments present in the skin of the grape. Much of the red wine production process involves extraction of color and flavor components from the grape skin.

Malolactic fermentation Process in winemaking

Malolactic conversion is a process in winemaking in which tart-tasting malic acid, naturally present in grape must, is converted to softer-tasting lactic acid. Malolactic fermentation is most often performed as a secondary fermentation shortly after the end of the primary fermentation, but can sometimes run concurrently with it. The process is standard for most red wine production and common for some white grape varieties such as Chardonnay, where it can impart a "buttery" flavor from diacetyl, a byproduct of the reaction.

Ethanol fermentation Biological process that produces ethanol and carbon dioxide as by-products

Ethanol fermentation, also called alcoholic fermentation, is a biological process which converts sugars such as glucose, fructose, and sucrose into cellular energy, producing ethanol and carbon dioxide as by-products. Because yeasts perform this conversion in the absence of oxygen, alcoholic fermentation is considered an anaerobic process. It also takes place in some species of fish where it provides energy when oxygen is scarce.

Maceration is the winemaking process where the phenolic materials of the grape—tannins, coloring agents (anthocyanins) and flavor compounds—are leached from the grape skins, seeds and stems into the must. To macerate is to soften by soaking, and maceration is the process by which the red wine receives its red color, since raw grape juice is clear-grayish in color. In the production of white wines, maceration is either avoided or allowed only in very limited manner in the form of a short amount of skin contact with the juice prior to pressing. This is more common in the production of varietals with less natural flavor and body structure like Sauvignon blanc and Sémillon. For Rosé, red wine grapes are allowed some maceration between the skins and must, but not to the extent of red wine production.

A wine fault or defect is an unpleasant characteristic of a wine often resulting from poor winemaking practices or storage conditions, and leading to wine spoilage. Many of the compounds that cause wine faults are already naturally present in wine but at insufficient concentrations to be of issue. In fact, depending on perception, these concentrations may impart positive characters to the wine. However, when the concentration of these compounds greatly exceeds the sensory threshold, they replace or obscure the flavors and aromas that the wine should be expressing. Ultimately the quality of the wine is reduced, making it less appealing and sometimes undrinkable.

Secondary fermentation is a process commonly associated with winemaking, which entails a second period of fermentation in a different vessel than the one used to start the fermentation process. An example of this would be starting fermentation in a carboy or stainless steel tank and then moving it over to oak barrels. Rather than being a separate, second fermentation, this is most often one single fermentation period that is conducted in multiple vessels. However, the term does also apply to procedures that could be described as a second and distinct fermentation period.

Outline of wine Overview of and topical guide to wine

The following outline is provided as an overview of and topical guide to wine

In brewing and winemaking, free amino nitrogen (FAN) is a measure of the concentration of individual amino acids and small peptides which can be utilized by beer and wine yeast for cell growth and proliferation. Together with ammonia, FAN makes up the measurement of yeast assimilable nitrogen that can be measured prior to the start of fermentation.

Fermentation in winemaking Wine making process

The process of fermentation in winemaking turns grape juice into an alcoholic beverage. During fermentation, yeasts transform sugars present in the juice into ethanol and carbon dioxide. In winemaking, the temperature and speed of fermentation are important considerations as well as the levels of oxygen present in the must at the start of the fermentation. The risk of stuck fermentation and the development of several wine faults can also occur during this stage, which can last anywhere from 5 to 14 days for primary fermentation and potentially another 5 to 10 days for a secondary fermentation. Fermentation may be done in stainless steel tanks, which is common with many white wines like Riesling, in an open wooden vat, inside a wine barrel and inside the wine bottle itself as in the production of many sparkling wines.

Governo is a winemaking technique reportedly invented in Tuscany in the 14th century to help complete fermentation and stabilize the wine. The technique involves saving a batch of harvested grapes and allowing them to partially dry. If fermentation of the main batch starts to slow or appears to be nearing stuck fermentation, the half dried grapes are added to the must which then gives the yeast cells a new source of sugar to enliven the batch. From there, the must can be fermented dry or stopped with the wine having a higher level of residual sugar. The process was widely used in the Chianti zones until the advent of temperature controlled fermentation tanks. From Tuscany the technique spread to Marche and Umbria where it is sometimes used today. In the Marche the technique is most often used on wines made from the Verdicchio grape to counteract the grape's natural bitterness and to add some sweetness and frizzante qualities.

Sugars in wine

Sugars in wine are at the heart of what makes winemaking possible. During the process of fermentation, sugars from wine grapes are broken down and converted by yeast into alcohol (ethanol) and carbon dioxide. Grapes accumulate sugars as they grow on the grapevine through the translocation of sucrose molecules that are produced by photosynthesis from the leaves. During ripening the sucrose molecules are hydrolyzed (separated) by the enzyme invertase into glucose and fructose. By the time of harvest, between 15 and 25% of the grape will be composed of simple sugars. Both glucose and fructose are six-carbon sugars but three-, four-, five- and seven-carbon sugars are also present in the grape. Not all sugars are fermentable, with sugars like the five-carbon arabinose, rhamnose and xylose still being present in the wine after fermentation. Very high sugar content will effectively kill the yeast once a certain (high) alcohol content is reached. For these reasons, no wine is ever fermented completely "dry". Sugar's role in dictating the final alcohol content of the wine sometimes encourages winemakers to add sugar during winemaking in a process known as chaptalization solely in order to boost the alcohol content – chaptalization does not increase the sweetness of a wine.

Acids in wine

The acids in wine are an important component in both winemaking and the finished product of wine. They are present in both grapes and wine, having direct influences on the color, balance and taste of the wine as well as the growth and vitality of yeast during fermentation and protecting the wine from bacteria. The measure of the amount of acidity in wine is known as the “titratable acidity” or “total acidity”, which refers to the test that yields the total of all acids present, while strength of acidity is measured according to pH, with most wines having a pH between 2.9 and 3.9. Generally, the lower the pH, the higher the acidity in the wine. There is no direct connection between total acidity and pH. In wine tasting, the term “acidity” refers to the fresh, tart and sour attributes of the wine which are evaluated in relation to how well the acidity balances out the sweetness and bitter components of the wine such as tannins. Three primary acids are found in wine grapes: tartaric, malic, and citric acids. During the course of winemaking and in the finished wines, acetic, butyric, lactic, and succinic acids can play significant roles. Most of the acids involved with wine are fixed acids with the notable exception of acetic acid, mostly found in vinegar, which is volatile and can contribute to the wine fault known as volatile acidity. Sometimes, additional acids, such as ascorbic, sorbic and sulfurous acids, are used in winemaking.

This glossary of winemaking terms lists some of terms and definitions involved in making wine, fruit wine, and mead.

Pressing (wine)

Pressing in winemaking is the process where the juice is extracted from the grapes with the aid of a wine press, by hand, or even by the weight of the grape berries and clusters. Historically, intact grape clusters were trodden by feet but in most wineries today the grapes are sent through a crusher/destemmer, which removes the individual grape berries from the stems and breaks the skins, releasing some juice, prior to being pressed. There are exceptions, such as the case of sparkling wine production in regions such as Champagne where grapes are traditionally whole-cluster pressed with stems included to produce a lighter must that is low in phenolics.

Clarification and stabilization of wine

In winemaking, clarification and stabilization are the processes by which insoluble matter suspended in the wine is removed before bottling. This matter may include dead yeast cells (lees), bacteria, tartrates, proteins, pectins, various tannins and other phenolic compounds, as well as pieces of grape skin, pulp, stems and gums. Clarification and stabilization may involve fining, filtration, centrifugation, flotation, refrigeration, pasteurization, and/or barrel maturation and racking.

Ripeness in viticulture

In viticulture, ripeness is the completion of the ripening process of wine grapes on the vine which signals the beginning of harvest. What exactly constitutes ripeness will vary depending on what style of wine is being produced and what the winemaker and viticulturist personally believe constitutes ripeness. Once the grapes are harvested, the physical and chemical components of the grape which will influence a wine's quality are essentially set so determining the optimal moment of ripeness for harvest may be considered the most crucial decision in winemaking.

Yeast in winemaking Yeasts used for alcoholic fermentation of wine

The role of yeast in winemaking is the most important element that distinguishes wine from grape juice. In the absence of oxygen, yeast converts the sugars of wine grapes into alcohol and carbon dioxide through the process of fermentation. The more sugars in the grapes, the higher the potential alcohol level of the wine if the yeast are allowed to carry out fermentation to dryness. Sometimes winemakers will stop fermentation early in order to leave some residual sugars and sweetness in the wine such as with dessert wines. This can be achieved by dropping fermentation temperatures to the point where the yeast are inactive, sterile filtering the wine to remove the yeast or fortification with brandy or neutral spirits to kill off the yeast cells. If fermentation is unintentionally stopped, such as when the yeasts become exhausted of available nutrients and the wine has not yet reached dryness, this is considered a stuck fermentation.

Yeast assimilable nitrogen Form of nitrogen available to wine yeast to use during fermentation

Yeast assimilable nitrogen or YAN is the combination of free amino nitrogen (FAN), ammonia (NH3) and ammonium (NH4+) that is available for the wine yeast Saccharomyces cerevisiae to use during fermentation. Outside of the fermentable sugars glucose and fructose, nitrogen is the most important nutrient needed to carry out a successful fermentation that doesn't end prior to the intended point of dryness or sees the development of off-odors and related wine faults. To this extent winemakers will often supplement the available YAN resources with nitrogen additives such as diammonium phosphate (DAP).

Fermented water Liquid composed of water and ethanol

Fermented water, is a liquid containing exclusively water and approximately 15–17% ethanol. The ethanol fermentation is produced by a mixture of refined sugar dissolved in water, which yeast is added to. Fermented water is formed when the yeast have consumed all the sugar, so it does not contain a sweet reserve which makes it taste completely dry. A refractometer can be used to control that it has zero must weight.

References

  1. 1 2 3 4 J. Robinson (ed) "The Oxford Companion to Wine" Third Edition pg 664-665 Oxford University Press 2006 ISBN   0-19-860990-6