The Trinidad Regional Virus Laboratory (T.R.V.L.) was established in Port of Spain, in 1953 by the Rockefeller Foundation in co-operation with the Government of Trinidad and Tobago. It was originally housed in an old wooden army barracks near the docks in Port of Spain. A large wired-in "animal house" was built out back to house the many wild animals brought in for study.
The Virus lab's first Director was the renowned epidemiologist, Dr Wilbur Downs who served in that role until 1961. In that year the laboratory was moved to new buildings at Federation Park, Port of Spain and, in 1964, became part of the Department of Microbiology of the University of the West Indies under the direction of Dr Leslie Spence, who had been with the laboratory since 1954. It is now part of the Caribbean Epidemiology Center (Carec) in Port of Spain.
The laboratory was one of four tropical virus research laboratories sponsored by the Rockefeller Foundation during the 1950s, including one in Brazil and two in Africa.
Under the inspired leadership of Dr Downs the laboratory began intensive research programs and made many new discoveries including the isolating of several arthropod-borne disease-causing viruses, and new insights into the epidemiology of key virus diseases including yellow fever, dengue fever, and rabies.
Downs headed a team of dedicated researchers including Dr. Charles R. Anderson (Virologist), Dr Leslie Spence (Epidemiologist), Dr Thomas Aitkens (Entomologist) and Dr Brooke Worth (Mammologist and Ornithologist). They worked very closely with other scientists in Trinidad, particularly those at the New York Zoological Society's research station headed by Dr William Beebe, the Imperial College of Tropical Agriculture, the Trinidad and Tobago Health and Agriculture Departments, Arthur M. Greenhall, a world expert on vampire bats, and Dr A. E. Hill, a specialist in Tropical and Internal Diseases, with a particular interest in Dengue fever.
The flourishing scientific community centered around the T.R.V.L., the New York Zoological Society's field station at Simla, and the Imperial College of Agriculture, provided an exciting pool of top-notch scholars whose interests often extended well past their immediate jobs. Much useful research was accomplished in these years in fields as diverse as archeology, the mating of butterflies and fiddler crabs, ornithology, and parasitology.
The laboratory also played host to constant stream of distinguishing visiting scientists from around the world, many of them conducting cutting-edge research in their fields, as well as photographers and illustrators from the National Geographic and other magazines, as well as providing first-class training and jobs for local people.
The discovery of a sick Red Howler monkey, (who was found to be suffering from yellow fever) in 1953 provided the first indication that yellow fever was still endemic in Trinidad although there had not been a case reliably reported from Trinidad since an outbreak in 1914.
It was discovered that a form of the disease "jungle yellow fever" was carried by Red Howler monkeys ( Alouatta macconnelli Elliot) who provided a continuous reservoir for the disease and spread by the Haemagogus s. spegazzini mosquito which normally inhabits rainforest regions, both at ground level and in the treetops.
After Government felling of large stands of native forest, yellow fever was isolated from a patient from Cumaca in the northern range in 1954. It soon began to spread to humans and be transmitted by the common Aedes aegypti mosquitoes.
Blood specimens were taken from over 4,500 humans in late 1953 and early 1954, and checked to detect the presence of a wide variety of known viruses. Over 15% showed antibodies to yellow fever and more human cases quickly followed.
Warnings were made that an epidemic was imminent and Downs and Hill began a program of inoculating health workers and stockpiling vaccine. Trinidad health authorities followed up with large-scale vaccination and intensive anti-aegypti measures including public education, regular inspection for breeding sites, and spraying of domestic residences with DDT. In spite of these measures, and the fact that an estimated 80% of the population of Port of Spain were immune to yellow fever and dengue, several more cases were soon reported. Most probably due to the health measures taken it did not develop into a widespread epidemic in Trinidad itself.
An attempt was made to totally quarantine the island just before Christmas, 1954, but the disease quickly spread to the nearby mainland of Venezuela and, from there, all the way to southern Mexico, probably killing several thousand people in the process.
Large-scale surveys were made of viruses and antigens in the local population, as well as domestic and wild animals. At the time the laboratory was established there were a number of common but unidentified disease-causing virus fevers in Trinidad, usually referred to by descriptive names such as "Trinidad 3-day fever", Trinidad 5-day fever", and the like. Some of these were soon isolated and identified.
A semi-permanent bush camp was set up at Bush Bush Wildlife Sanctuary in the large Nariva Swamp in southeastern Trinidad and a large tree station was built in the Vega de Oropouche rainforest near Sangre Grande with platforms at 60, 90 and 120 feet (18, 27 and 36.6 metres) to facilitate collecting mosquitoes at various levels in the rainforest, including the forest canopy.
Dr. H. Metivier, a Veterinary Surgeon, who established in 1931 the connection between the bites of bats and paralytic rabies, and Dr. J. L. Pawan, a Government Bacteriologist found Negri bodies in the brain of a bat with unusual habits in September 1931, finally demonstrated that rabies could be transmitted to humans by the infected saliva of vampire bats. In 1934, the Government began a program of vampire bat control, shooting, netting and trapping, while encouraging the screening off of livestock buildings and free vaccination programs for exposed livestock.
After the opening of the Virus laboratory in 1953 basic research on bats and the transmission of rabies progressed rapidly under the able direction of Arthur Greenhall, a Government Zoologist.
Some of the main viruses isolated at T.R.V.L. (new discoveries marked with an asterisk):
Yellow fever is a viral disease of typically short duration. In most cases, symptoms include fever, chills, loss of appetite, nausea, muscle pains particularly in the back, and headaches. Symptoms typically improve within five days. In about 15% of people, within a day of improving the fever comes back, abdominal pain occurs, and liver damage begins causing yellow skin. If this occurs, the risk of bleeding and kidney problems is increased.
Dengue fever is a mosquito-borne tropical disease caused by the dengue virus. Symptoms typically begin three to fourteen days after infection. These may include a high fever, headache, vomiting, muscle and joint pains, and a characteristic skin rash. Recovery generally takes two to seven days. In a small proportion of cases, the disease develops into severe dengue, also known as dengue hemorrhagic fever, resulting in bleeding, low levels of blood platelets and blood plasma leakage, or into dengue shock syndrome, where dangerously low blood pressure occurs.
Arbovirus is an informal name used to refer to any viruses that are transmitted by arthropod vectors. The word arbovirus is an acronym. The word tibovirus is sometimes used to more specifically describe viruses transmitted by ticks, a superorder within the arthropods. Arboviruses can affect both animals and plants. In humans, symptoms of arbovirus infection generally occur 3–15 days after exposure to the virus and last three or four days. The most common clinical features of infection are fever, headache, and malaise, but encephalitis and viral hemorrhagic fever may also occur.
Max Theiler was a South African-American virologist and physician. He was awarded the Nobel Prize in Physiology or Medicine in 1951 for developing a vaccine against yellow fever in 1937, becoming the first African-born Nobel laureate.
The Nariva Swamp is the largest freshwater wetland in Trinidad and Tobago and has been designated a Wetland of International Importance under the Ramsar Convention. The swamp is located on the east coast of Trinidad, immediately inland from the Manzanilla Bay through Biche and covers over 60 square kilometres (23 mi2). The Nariva Swamp is extremely biodiverse. It is home to 45 mammal species, 39 reptile species, 33 fish species, 204 bird species, 19 frog species, 213 insect species and 15 mollusc species. All this contained in just 60 square kilometers.
Australian bat lyssavirus (ABLV), initially named Pteropid lyssavirus (PLV), is a zoonotic virus closely related to the rabies virus. It was first identified in a 5-month-old juvenile black flying fox collected near Ballina in northern New South Wales, Australia, in January 1995 during a national surveillance program for the recently identified Hendra virus. ABLV is the seventh member of the genus Lyssavirus and the only Lyssavirus member present in Australia.
Oropouche fever is a tropical viral infection transmitted by biting midges and mosquitoes from the blood of sloths to humans. This disease is named after the region where it was first discovered and isolated at the Trinidad Regional Virus Laboratory in 1955 by the Oropouche River in Trinidad and Tobago. Oropouche fever is caused by a specific arbovirus, the Oropouche virus (OROV), of the Bunyaviridae family.
Oropouche orthobunyavirus (OROV) is one of the most common orthobunyaviruses. When OROV infects humans, it causes a rapid fever illness called Oropouche fever. OROV was originally reported in Trinidad and Tobago in 1955 from the blood sample of a fever patient and from a pool of Coquillettidia venezuelensis mosquitoes. In 1960, OROV was isolated from a sloth and a pool of Ochlerotatus (Ochlerotatus) serratus mosquitoes in Brazil. The virus is considered a public health threat in tropical and subtropical areas of Central and South America, with over half million infected people as of 2005. OROV is considered to be an arbovirus due to the method of transmission by the mosquitoes Aedes serratus and Culex quinquefasciatus among sloths, marsupials, primates, and birds.
Wilbur George Downs, was a naturalist, virologist and clinical professor of epidemiology and public health at the Yale School of Medicine and the Yale School of Public Health.
Greenhall's dog-faced bat is a South American bat species of the family Molossidae. It is found in Colombia, Peru, Ecuador, Venezuela, the Guianas, northeastern Brazil and Trinidad.
The National Institute of Virology, Pune is an Indian virology research institute, and one of the translational science cells part of Indian Council of Medical Research (ICMR). It was previously known as 'Virus Research Center' and was founded in collaboration with the Rockefeller Foundation. It has been designated as a WHO H5 reference laboratory for SE Asia region.
Rabies is a viral zoonotic neuroinvasive disease which causes inflammation in the brain and is usually fatal. Rabies, caused by the rabies virus, primarily infects mammals. In the laboratory it has been found that birds can be infected, as well as cell cultures from birds, reptiles and insects. Animals with rabies suffer deterioration of the brain and tend to behave bizarrely and often aggressively, increasing the chances that they will bite another animal or a person and transmit the disease. Most cases of humans contracting the disease from infected animals are in developing nations. In 2010, an estimated 26,000 people died from rabies, down from 54,000 in 1990.
Mosquito-borne diseases or mosquito-borne illnesses are diseases caused by bacteria, viruses or parasites transmitted by mosquitoes. Nearly 700 million people get a mosquito-borne illness each year resulting in over one million deaths.
Haemagogus is a genus of mosquitoes in the dipteran family Culicidae. They mainly occur in Central America and northern South America, although some species inhabit forested areas of Brazil, and range as far as northern Argentina. In the Rio Grande Do Sul area of Brazil, one species, H. leucocelaenus, has been found carrying yellow fever virus. Several species have a distinct metallic sheen.
Joseph Lennox Donation Pawan MBE was a Trinidadian bacteriologist who was the first person to show that rabies could be spread by vampire bats to other animals and humans.
Robert Ellis Shope was an American virologist, epidemiologist and public health expert, particularly known for his work on arthropod-borne viruses and emerging infectious diseases. He discovered more novel viruses than any person previously, including members of the Arenavirus, Hantavirus, Lyssavirus and Orbivirus genera of RNA viruses. He researched significant human diseases, including dengue, Lassa fever, Rift Valley fever, yellow fever, viral hemorrhagic fevers and Lyme disease. He had an encyclopedic knowledge of viruses, and curated a global reference collection of over 5,000 viral strains. He was the lead author of a groundbreaking report on the threat posed by emerging infectious diseases, and also advised on climate change and bioterrorism.
John ("Jack") Payne Woodall (1935–2016) was a British/American entomologist and virologist who made significant contributions to the study of arboviruses in South America, the Caribbean and Africa. He did research on the causative agents of dengue fever, Crimean–Congo hemorrhagic fever, o'nyong'nyong fever, yellow fever, Zika fever, and others. He served as a staff member of the Rockefeller Foundation, and director of the Foundation's laboratory in Brazil, as a research fellow at the Yale Arbovirus Research Unit, was head of the Arbovirus Laboratory for the New York State Health Department, and worked for the Centers for Disease Control. Woodall spent 13 years at the World Health Organization developing and evaluating health programs. After retirement in 2007, he continued as a consultant and professor at the Institute of Medical Biochemistry in Rio de Janeiro, Brazil, where he had worked since 1998. In 1994, he cofounded the Program for Monitoring Emerging Diseases (ProMED-mail). Woodall's emails concluded with a quote from the Calvin and Hobbes cartoon, "God put me on this earth to accomplish a certain number of things. Right now I'm so far behind I will never die."
A notifiable disease is one which the law requires to be reported to government authorities.
Jordi Casals i Ariet was a Catalan physician and epidemiologist.
Scott Halstead is an American physician-scientist, virologist and epidemiologist known for his work in the fields of tropical medicine and vaccine development. He is considered one of the world’s foremost authorities on viruses transmitted by mosquitoes, including Dengue, Japanese encephalitis, chikungunya and Zika. He was one of the first researchers to identify the phenomenon known as antibody-dependent enhancement (ADE), where the antibodies generated from a first dengue infection can sometimes worsen the symptoms from a second infection.