Triple smear

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Triple smear
Synonyms Vaginal-cervical-endocervical smear
Test ofSamples of the ectocervix, vagina, and endocervix

Triple smear, also known as vaginal-cervical-endocervical (VCE) smear is a cytopathology technique for identifying lesions of the female genital tract. The smear is prepared on separate areas of a single slide with three distinct samples, each from ectocervix, vagina and endocervix. [1] Each sample occupies one-third of the glass slide. This method requires expertise as three samples need to be collected rapidly to avoid drying up of the smear. Triple smear is recommended in patients who had a previous abnormal smear (or endometrial debris in previous smear in a climacteric woman), in postmenopausal women, women with perimenopausal bleeding and those with high risk for endometrial carcinoma. [2]

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Tzanck test

In dermatopathology, the Tzanck test, also Tzanck smear, is scraping of an ulcer base to look for Tzanck cells. It is sometimes also called the chickenpox skin test and the herpes skin test. It is a simple, low-cost, and rapid office based test.

Endometrial hyperplasia Medical condition

Endometrial hyperplasia is a condition of excessive proliferation of the cells of the endometrium, or inner lining of the uterus.

Endometrial biopsy

The endometrial biopsy is a medical procedure that involves taking a tissue sample of the lining of the uterus. The tissue subsequently undergoes a histologic evaluation which aids the physician in forming a diagnosis.

The Tao brush is a medical instrument used to perform an alternative method of endometrial biopsy, for the purpose of detecting endometrial cancer.

Cervical polyp Medical condition

A cervical polyp is a common benign polyp or tumour on the surface of the cervical canal. They can cause irregular menstrual bleeding but often show no symptoms. Treatment consists of simple removal of the polyp and prognosis is generally good. About 1% of cervical polyps will show neoplastic change which may lead to cancer. They are most common in post-menarche, pre-menopausal women who have been pregnant.

Vaginal wet mount

A vaginal wet mount is a gynecologic test wherein a sample of vaginal discharge is observed by wet mount microscopy by placing the specimen on a glass slide and mixing with a salt solution. It is used to find the cause of vaginitis and vulvitis.

Cervical screening

Cervical screening is the process of detecting and removing abnormal tissue or cells in the cervix before cervical cancer develops. By aiming to detect and treat cervical neoplasia early on, cervical screening aims at secondary prevention of cervical cancer. Several screening methods for cervical cancer are the Pap test, liquid-based cytology, the HPV DNA testing and the visual inspection with acetic acid. Pap test and liquid-based cytology have been effective in diminishing incidence and mortality rates of cervical cancer in developed countries but not in developing countries. Prospective screening methods that can be used in low-resource areas in developing countries are the HPV DNA testing and the visual inspection.

Liquid-based cytology Method of preparing samples for cytopathology

Liquid-based cytology is a method of preparing samples for examination in cytopathology. The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid. At the laboratory, the liquid is treated to remove other elements such as mucus before a layer of cells is placed on a slide. The technique improves specimen adequacy and HSIL detection. The United Kingdom screening programmes changed their cervical screening method from the Pap test to liquid-based cytology in 2008.


  1. Harshmohan (2014-10-31). Harshmohan's Textbook of Pathology. New Delhi: Jaypee Publications. p. 268. ISBN   978-9351523697.
  2. Krunger, TF; Botha, MH (2007). Clinical gynaecology (3 ed.). South Africa: Juta. p. 23. ISBN   9780702173059 . Retrieved 7 December 2016.