Azotobacter chroococcum

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Azotobacter chroococcum
Corn.Field.jpg
Image of a corn field on which A. chroococcum could be used as a biofertilizer
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Domain: Bacteria
Phylum: Pseudomonadota
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Azotobacter
Species:
A. chroococcum
Binomial name
Azotobacter chroococcum
Beijerinck 1901 [1]

Azotobacter chroococcum is a bacterium that has the ability to fix atmospheric nitrogen. It was discovered by Martinus Beijerinck in 1901, and was the first aerobic, free-living nitrogen fixer discovered. [2] A. chroococcum could be useful for nitrogen fixation in crops as a biofertilizer, fungicide, and nutrient indicator, and in bioremediation.

Contents

Characteristics

A. chroococcum is a microaerophilic plant growth-promoting rhizobacterium (PGRP), [3] [4] which is bacillus in shape and is Gram negative. [4] As a mesophile, this bacterium grows best in moderate-temperature soils and requires a neutral pH environment.[ citation needed ] It is able to fix nitrogen under aerobic conditions. [3] The soil cannot be poor in phosphorus or else nitrogen fixing can be hindered. In addition to phosphorus, these bacteria needed potassium, "sulphur, magnesium, and calcium" to grow.[ citation needed ] To fix nitrogen A. chroococcum produces three enzymes (catalase, peroxidase, and superoxide dismutase) to "neutralise" reactive oxygen species. It also forms the dark-brown, water-soluble pigment melanin at high levels of metabolism during the fixation of nitrogen, which is thought to protect the nitrogenase system from oxygen. [3] In the presence of some saccharides (such as sucrose and raffinose) while on agar plates, a levan ring can form around the A. chroococcum colony. [5]

Uses

Research has been carried out into A. chroococcum's potential applications in improving crop production. At least one study has so far shown a significant increase in crop production linked to the production of "auxins, cytokinins, and GA–like substances" by A. chroococcum. [6] In addition to these biomolecules, this bacterium has been found to be able to produce "siderophores, ammonia, and ACC deaminase", as well as indoleacetic acid, which all are known to assist with the growth of crops. [4]

On top of helping with the growth of crops in general, A. chroococcum has also been shown to help crops grow in polluted soils. A. chroococcum is able to survive and improve the growth of crops in soils polluted with heavy metals when seeds are inoculated with the bacterium prior to planting. [4] A. chroococcum not only produced growth-positive biomolecules and increased the number and quality of maize kernels, but also reduced the production of "proline, antioxidant enzymes, and MDA" in the plants, all which indicate of the presence of heavy metals in the soil. [4]

In addition to treating soils polluted with heavy metals, A. chroococcum can act as a fungicide that can be used to treat soils and plants inflicted with fungal infections, specifically fungi that are susceptible to "fungicidal substances of the anisomycin group".[ citation needed ]

This bacterium can also be used to determine the nutrient composition of the soil. Since plants and A.chroccoccum both need phosphorus and potassium to grow, this bacterium can be used to determine if the soil is fit for crop growth, as it would thrive in soils that have these nutrients.[ citation needed ]

A. chroococcum is also a possible asset for bioremediation. Melanin produced by this bacterium can bind to heavy metals, subsequently protecting A. chroococcum, which may be useful for removing heavy metals from polluted soils. [4] Since this bacterium is able promote plant growth through nitrogen fixation, it can also reduce the amount of nitrogen that has to be added to fields, which can reduce the amount of nitrogen runoff. This effect of less nitrogen being added in soil was seen in a study involving cotton plants. [7]

Related Research Articles

Nitrogen fixation is a chemical process by which molecular nitrogen (N
2
), which has a strong triple covalent bond, is converted into ammonia (NH
3
) or related nitrogenous compounds, typically in soil or aquatic systems but also in industry. The nitrogen in air is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms. Biological nitrogen fixation or diazotrophy is an important microbe-mediated process that converts dinitrogen (N2) gas to ammonia (NH3) using the nitrogenase protein complex (Nif).

<span class="mw-page-title-main">Fertilizer</span> Substance added to soils to supply plant nutrients for a better growth

A fertilizer or fertiliser is any material of natural or synthetic origin that is applied to soil or to plant tissues to supply plant nutrients. Fertilizers may be distinct from liming materials or other non-nutrient soil amendments. Many sources of fertilizer exist, both natural and industrially produced. For most modern agricultural practices, fertilization focuses on three main macro nutrients: nitrogen (N), phosphorus (P), and potassium (K) with occasional addition of supplements like rock flour for micronutrients. Farmers apply these fertilizers in a variety of ways: through dry or pelletized or liquid application processes, using large agricultural equipment or hand-tool methods.

<span class="mw-page-title-main">Cover crop</span> Crop planted to manage erosion and soil quality

In agriculture, cover crops are plants that are planted to cover the soil rather than for the purpose of being harvested. Cover crops manage soil erosion, soil fertility, soil quality, water, weeds, pests, diseases, biodiversity and wildlife in an agroecosystem—an ecological system managed and shaped by humans. Cover crops can increase microbial activity in the soil, which has a positive effect on nitrogen availability, nitrogen uptake in target crops, and crop yields. Cover crops may be an off-season crop planted after harvesting the cash crop. Cover crops are nurse crops in that they increase the survival of the main crop being harvested, and are often grown over the winter. In the United States, cover cropping may cost as much as $35 per acre.

<span class="mw-page-title-main">Plant nutrition</span> Study of the chemical elements and compounds necessary for normal plant life

Plant nutrition is the study of the chemical elements and compounds necessary for plant growth and reproduction, plant metabolism and their external supply. In its absence the plant is unable to complete a normal life cycle, or that the element is part of some essential plant constituent or metabolite. This is in accordance with Justus von Liebig’s law of the minimum. The total essential plant nutrients include seventeen different elements: carbon, oxygen and hydrogen which are absorbed from the air, whereas other nutrients including nitrogen are typically obtained from the soil.

Diazotrophs are bacteria and archaea that fix gaseous nitrogen in the atmosphere into a more usable form such as ammonia.

<i>Azotobacter</i> Genus of bacteria

Azotobacter is a genus of usually motile, oval or spherical bacteria that form thick-walled cysts and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil. In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, Azotobacter chroococcum, was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. Azotobacter species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants.

Azotobacter vinelandii is Gram-negative diazotroph that can fix nitrogen while grown aerobically. These bacteria are easily cultured and grown.

<span class="mw-page-title-main">Organic fertilizer</span> Fertilizer developed from natural processes

Organic fertilizers are fertilizers that are naturally produced. Fertilizers are materials that can be added to soil or plants, in order to provide nutrients and sustain growth. Typical organic fertilizers include all animal waste including meat processing waste, manure, slurry, and guano; plus plant based fertilizers such as compost; and biosolids. Inorganic "organic fertilizers" include minerals and ash. The organic-mess refers to the Principles of Organic Agriculture, which determines whether a fertilizer can be used for commercial organic agriculture, not whether the fertilizer consists of organic compounds.

<span class="mw-page-title-main">Rhizobacteria</span> Group of bacteria affecting plant growth

Rhizobacteria are root-associated bacteria that can have a detrimental, neutral or beneficial effect on plant growth. The name comes from the Greek rhiza, meaning root. The term usually refers to bacteria that form symbiotic relationships with many plants (mutualism). Rhizobacteria are often referred to as plant growth-promoting rhizobacteria, or PGPRs. The term PGPRs was first used by Joseph W. Kloepper in the late 1970s and has become commonly used in scientific literature.

<span class="mw-page-title-main">Phototrophic biofilm</span> Microbial communities including microorganisms which use light as their energy source

Phototrophic biofilms are microbial communities generally comprising both phototrophic microorganisms, which use light as their energy source, and chemoheterotrophs. Thick laminated multilayered phototrophic biofilms are usually referred to as microbial mats or phototrophic mats. These organisms, which can be prokaryotic or eukaryotic organisms like bacteria, cyanobacteria, fungi, and microalgae, make up diverse microbial communities that are affixed in a mucous matrix, or film. These biofilms occur on contact surfaces in a range of terrestrial and aquatic environments. The formation of biofilms is a complex process and is dependent upon the availability of light as well as the relationships between the microorganisms. Biofilms serve a variety of roles in aquatic, terrestrial, and extreme environments; these roles include functions which are both beneficial and detrimental to the environment. In addition to these natural roles, phototrophic biofilms have also been adapted for applications such as crop production and protection, bioremediation, and wastewater treatment.

Microbial inoculants also known as soil inoculants or bioinoculants are agricultural amendments that use beneficial rhizosphericic or endophytic microbes to promote plant health. Many of the microbes involved form symbiotic relationships with the target crops where both parties benefit (mutualism). While microbial inoculants are applied to improve plant nutrition, they can also be used to promote plant growth by stimulating plant hormone production. Although bacterial and fungal inoculants are common, inoculation with archaea to promote plant growth is being increasingly studied.

<span class="mw-page-title-main">Agricultural pollution</span> Type of pollution caused by agriculture

Agricultural pollution refers to biotic and abiotic byproducts of farming practices that result in contamination or degradation of the environment and surrounding ecosystems, and/or cause injury to humans and their economic interests. The pollution may come from a variety of sources, ranging from point source water pollution to more diffuse, landscape-level causes, also known as non-point source pollution and air pollution. Once in the environment these pollutants can have both direct effects in surrounding ecosystems, i.e. killing local wildlife or contaminating drinking water, and downstream effects such as dead zones caused by agricultural runoff is concentrated in large water bodies.

<span class="mw-page-title-main">Biofertilizer</span> Substance with micro-organisms

A biofertilizer is a substance which contains living micro-organisms which, when applied to seeds, plant surfaces, or soil, colonize the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers add nutrients through the natural processes of nitrogen fixation, solubilizing phosphorus, and stimulating plant growth through the synthesis of growth-promoting substances. The micro-organisms in biofertilizers restore the soil's natural nutrient cycle and build soil organic matter. Through the use of biofertilizers, healthy plants can be grown, while enhancing the sustainability and the health of the soil. Biofertilizers can be expected to reduce the use of synthetic fertilizers and pesticides, but they are not yet able to replace their use. Since they play several roles, a preferred scientific term for such beneficial bacteria is "plant-growth promoting rhizobacteria" (PGPR).

Agricultural microbiology is a branch of microbiology dealing with plant-associated microbes and plant and animal diseases. It also deals with the microbiology of soil fertility, such as microbial degradation of organic matter and soil nutrient transformations.

Azotobacter salinestris is a Gram-negative, nitrogen-fixing bacterium; its specific name, salinestris, comes from the Latin words salinus meaning saline and estris which means "living in". It can be found living in soil or marine habitats as single cells or in chains of six to eight cells. This organism is motile at younger stages, but loses its flagella at older stages. This species is known for its potential use in bioremediation.

Azospirillum is a Gram-negative, microaerophilic, non-fermentative and nitrogen-fixing bacterial genus from the family of Rhodospirillaceae. Azospirillum bacteria can promote plant growth.

<span class="mw-page-title-main">Mycorrhiza helper bacteria</span> Group of organisms

Mycorrhiza helper bacteria (MHB) are a group of organisms that form symbiotic associations with both ectomycorrhiza and arbuscular mycorrhiza. MHBs are diverse and belong to a wide variety of bacterial phyla including both Gram-negative and Gram-positive bacteria. Some of the most common MHBs observed in studies belong to the phylas Pseudomonas and Streptomyces. MHBs have been seen to have extremely specific interactions with their fungal hosts at times, but this specificity is lost with plants. MHBs enhance mycorrhizal function, growth, nutrient uptake to the fungus and plant, improve soil conductance, aid against certain pathogens, and help promote defense mechanisms. These bacteria are naturally present in the soil, and form these complex interactions with fungi as plant root development starts to take shape. The mechanisms through which these interactions take shape are not well-understood and needs further study.

Some types of lichen are able to fix nitrogen from the atmosphere. This process relies on the presence of cyanobacteria as a partner species within the lichen. The ability to fix nitrogen enables lichen to live in nutrient-poor environments. Lichen can also extract nitrogen from the rocks on which they grow.

Seventeen elements or nutrients are essential for plant growth and reproduction. They are carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), potassium (K), sulfur (S), calcium (Ca), magnesium (Mg), iron (Fe), boron (B), manganese (Mn), copper (Cu), zinc (Zn), molybdenum (Mo), nickel (Ni) and chlorine (Cl). Nutrients required for plants to complete their life cycle are considered essential nutrients. Nutrients that enhance the growth of plants but are not necessary to complete the plant's life cycle are considered non-essential, although some of them, such as silicon (Si), have been shown to improve nutrent availability, hence the use of stinging nettle and horsetail macerations in Biodynamic agriculture. With the exception of carbon, hydrogen and oxygen, which are supplied by carbon dioxide and water, and nitrogen, provided through nitrogen fixation, the nutrients derive originally from the mineral component of the soil. The Law of the Minimum expresses that when the available form of a nutrient is not in enough proportion in the soil solution, then other nutrients cannot be taken up at an optimum rate by a plant. A particular nutrient ratio of the soil solution is thus mandatory for optimizing plant growth, a value which might differ from nutrient ratios calculated from plant composition.

Seaweed fertiliser is organic fertilizer made from seaweed that is used in agriculture to increase soil fertility and plant growth. The use of seaweed fertilizer dates back to antiquity and has a broad array of benefits for soils. Seaweed fertilizer can be applied in a number of different forms, including refined liquid extracts and dried, pulverized organic material. Through its composition of various bioactive molecules, seaweed functions as a strong soil conditioner, bio-remediator, and biological pest control, with each seaweed phylum offering various benefits to soil and crop health. These benefits can include increased tolerance to abiotic stressors, improved soil texture and water retention, and reduced occurrence of diseases.

References

  1. Parte, A.C. "Azotobacter". LPSN .
  2. Beijerinck M. W. (1901). "Ueber Oligonitrophile Mikroben". Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abteilung II (in German) (7): 561–582.
  3. 1 2 3 Shivprasad S., Page W. J. (1989). "Catechol Formation and Melanization by Na+ -Dependent Azotobacter chroococcum: a Protective Mechanism for Aeroadaptation?". Applied and Environmental Microbiology. 55 (7): 1811–1817. Bibcode:1989ApEnM..55.1811S. doi:10.1128/AEM.55.7.1811-1817.1989. PMC   202955 . PMID   16347974.
  4. 1 2 3 4 5 6 Rizvi, Asfa; Kahn, Mohd. Saghir (August 15, 2018). "Heavy metal induced oxidative damage and root morphology alterations of maize (Zea mays L.) plants and stress mitigation by metal tolerant nitrogen fixing Azotobacter chroococcum". Ecotoxicology and Environmental Safety. 157: 9–20. doi:10.1016/j.ecoenv.2018.03.063. PMID   29605647. S2CID   4552954.
  5. Robson, Robert L.; Jones, Robert; Robson, R. Moyra; Schwartz, Ariel; Richardson, Toby H. (June 10, 2015). "Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003 (ATCC 4412)". PLOS ONE. 10 (6): 5–6. Bibcode:2015PLoSO..1027997R. doi: 10.1371/journal.pone.0127997 . PMC   4465626 . PMID   26061173.
  6. Wani, Sartaj; Chand, Subhash; Ali, Tahir (29 August 2013). "Potential Use of Azotobacter chroococcum in Crop Production: An Overview". Current Agriculture Research Journal. 1 (1): 35–38. doi: 10.12944/CARJ.1.1.04 .
  7. Wang, Juanjuan; Li, Ruochen; Zhang, Hui; Wei, Gehong; Li, Zhefei (2020-02-21). "Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application". BMC Microbiology. 20 (1): 38. doi: 10.1186/s12866-020-1708-z . ISSN   1471-2180. PMC   7035779 . PMID   32085752.