Carboxylesterase 1

CES1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1MX1, 1MX5, 1MX9, 1YA4, 1YA8, 1YAH, 1YAJ, 2DQY, 2DQZ, 2DR0, 2H7C, 2HRQ, 2HRR, 3K9B, 4AB1, 5A7F, 5A7H, 5A7G
Identifiers
Aliases	CES1 , ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1, carboxylesterase 1
External IDs	OMIM: 114835 MGI: 2148202 HomoloGene: 35606 GeneCards: CES1
EC number	3.1.1.1
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16q12.2 Start 55,802,851 bp [1] End 55,833,337 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 C5 Start 93,892,696 bp [2] End 93,924,466 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right lobe of liver gallbladder right lung upper lobe of left lung smooth muscle tissue prostate popliteal artery spleen subcutaneous adipose tissue left uterine tube Top expressed in white adipose tissue lacrimal gland gallbladder right lung lobe olfactory epithelium superior surface of tongue brown adipose tissue left lung left lung lobe subcutaneous adipose tissue More reference expression data BioGPS n/a
Gene ontology Molecular function methylumbelliferyl-acetate deacetylase activity carboxylic ester hydrolase activity hydrolase activity sterol esterase activity triglyceride lipase activity methyl indole-3-acetate esterase activity Cellular component endoplasmic reticulum lumen cytosol endoplasmic reticulum extracellular space Biological process xenobiotic metabolic process epithelial cell differentiation response to toxic substance cholesterol ester hydrolysis involved in cholesterol transport cholesterol biosynthetic process medium-chain fatty acid metabolic process lipid catabolic process angiotensin maturation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1066 104158 Ensembl ENSG00000198848 ENSG00000262243 ENSMUSG00000056973 UniProt P23141 Q8VCT4 RefSeq (mRNA) NM_001025194 NM_001025195 NM_001266 NM_053200 RefSeq (protein) NP_001020365 NP_001020366 NP_001257 NP_444430 Location (UCSC) Chr 16: 55.8 – 55.83 Mb Chr 8: 93.89 – 93.92 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. ^{[5] [6]} The protein is also historically known as serine esterase 1 (SES1), monocyte esterase and cholesterol ester hydrolase (CEH). Three transcript variants encoding three different isoforms have been found for this gene. ^[6] The various protein products from isoform a, b and c range in size from 568, 567 and 566 amino acids long, respectively.

CES1 is present in most tissues with higher levels in the liver and low levels in the gastrointestinal tract. ^[7]

Function

Carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. It is also part of the alpha/beta fold hydrolase family. ^[7] These enzymes are responsible for the hydrolysis of ester- and amide-bond-containing xenobiotics and drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. As part of phase II metabolism, the resulting carboxylates are then often conjugated by other enzymes to increase solubility and eventually excreted.

This enzyme is known to hydrolyze aromatic and aliphatic esters and can manage cellular cholesterol esterification levels. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds. ^[6]

The protein contains an amino acid sequence at its N-terminus that sends it into the endoplasmic reticulum where a C-terminal sequence can bind to a KDEL receptor. ^[7]

Clinical significance

Carboxylesterase 1 deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. Inhibition of CES1 by in particular organophosphates reduces tumor-killing activity by monocytes. ^{[8] [9]} The loss of this protein in monocytes is one product of organophosphate poisoning. ^[9]

CES1 can activate or deactivate various drugs. CES1 is responsible for the activation of many prodrugs such as angiotensin-converting enzyme (ACE) inhibitors, oseltamivir, and dabigatran. ^{[10] [11] [12] [13]} Genetic variants of CES1 can significantly affect both pharmacokinetics and pharmacodynamics of drugs metabolized by CES1, such as methylphenidate and clopidogrel. ^[14] The ability of CES1 to metabolize heroin and cocaine among other drugs has suggested a therapeutic role for the enzyme. ^[15]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. [§ 1] [[File: [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] |alt=Fluorouracil (5-FU) Activity edit]] Fluorouracil (5-FU) Activity edit The interactive pathway map can be edited at WikiPathways: "FluoropyrimidineActivity_WP1601".

Click on genes, proteins and metabolites below to link to respective articles. [§ 1] [[File: [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] [[ ]] |alt=Irinotecan Pathway edit]] Irinotecan Pathway edit The interactive pathway map can be edited at WikiPathways: "IrinotecanPathway_WP229".

References

1. ENSG00000262243 GRCh38: Ensembl release 89: ENSG00000198848, ENSG00000262243 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000056973 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Zschunke F, Salmassi A, Kreipe H, Buck F, Parwaresch MR, Radzun HJ (Aug 1991). "cDNA cloning and characterization of human monocyte/macrophage serine esterase-1". Blood. 78 (2): 506–12. doi: 10.1182/blood.V78.2.506.506 . PMID   2070086.
6. "Entrez Gene: CES1 carboxylesterase 1 (monocyte/macrophage serine esterase 1)".
7. Imai T (Jun 2006). "Human Carboxylesterase Isozymes: Catalytic Properties and Rational Drug Design". Drug Metabolism and Pharmacokinetics. 21 (3): 173–185. doi:10.2133/dmpk.21.173. PMID   16858120.
8. Oertel J, Hagner G, Kastner M, Huhn D (Dec 1985). "The relevance of alpha-naphthyl acetate esterases to various monocyte functions". Br J Haematol. 61 (4): 717–26. doi:10.1111/j.1365-2141.1985.tb02886.x. PMID   4084460. S2CID   84899499.
9. Markey GM (Feb 2011). "Carboxylesterase 1 (Ces1): from monocyte marker to major player". J Clin Pathol. 64 (2): 107–9. doi:10.1136/jcp.2010.084657. PMID   21177752. S2CID   32622794.
10. Zhu HJ, Appel DI, Johnson JA, Chavin KD, Markowitz JS (Jan 2009). "Role of carboxylesterase 1 and impact of natural genetic variants on the hydrolysis of trandolapril". Biochem Pharmacol. 77 (7): 1266–72. doi:10.1016/j.bcp.2008.12.017. PMID   19185566.
11. Thomsen R, Rasmussen HB, Linnet K, INDICES Consortium (Jan 2014). "In vitro drug metabolism by human carboxylesterase 1: focus on angiotensin-converting enzyme inhibitors". Drug Metab Dispos. 42 (1): 126–33. doi:10.1124/dmd.113.053512. PMID   24141856. S2CID   206496779.
12. Zhu HJ, Markowitz JS (Feb 2009). "Activation of the antiviral prodrug oseltamivir is impaired by two newly identified carboxylesterase 1 variants". Drug Metab Dispos. 37 (2): 264–7. doi:10.1124/dmd.108.024943. PMID   19022936. S2CID   9277216.
13. Paré G, Eriksson N, Lehr T, Connolly S, Eikelboom J, Ezekowitz MD, Axelsson T, Haertter S, Oldgren J, Reilly P, Siegbahn A, Syvanen AC, Wadelius C, Wadelius M, Zimdahl-Gelling H, Yusuf S, Wallentin L (Apr 2013). "Genetic determinants of dabigatran plasma levels and their relation to bleeding". Circulation. 127 (13): 1404–12. doi: 10.1161/CIRCULATIONAHA.112.001233 . PMID   23467860.
14. Zhu HJ, Patrick KS, Yuan HJ, Wang JS, Donovan JL, DeVane CL, Malcolm R, Johnson JA, Youngblood GL, Sweet DH, Langaee TY, Markowitz JS (Jun 2008). "Two CES1 gene mutations lead to dysfunctional carboxylesterase 1 activity in man: clinical significance and molecular basis". Am J Hum Genet. 82 (6): 1241–8. doi:10.1016/j.ajhg.2008.04.015. PMC   2427248 . PMID   18485328.
15. Redinbo MR, Bencharit S, Potter PM (Jun 2003). "Human carboxylesterase 1: from drug metabolism to drug discovery". Biochem Soc Trans. 31 (Pt 3): 620–4. doi:10.1042/bst0310620. PMID   12773168.

Further reading

• Riddles PW, Richards LJ, Bowles MR, Pond SM (1992). "Cloning and analysis of a cDNA encoding a human liver carboxylesterase". Gene. 108 (2): 289–92. doi:10.1016/0378-1119(91)90448-K. PMID   1748313.
• Munger JS, Shi GP, Mark EA, et al. (1991). "A serine esterase released by human alveolar macrophages is closely related to liver microsomal carboxylesterases". J. Biol. Chem. 266 (28): 18832–8. doi: 10.1016/S0021-9258(18)55139-5 . PMID   1918003.
• Long RM, Calabrese MR, Martin BM, Pohl LR (1991). "Cloning and sequencing of a human liver carboxylesterase isoenzyme". Life Sci. 48 (11): PL43–9. doi:10.1016/0024-3205(91)90515-D. PMID   1997784.
• Ketterman AJ, Bowles MR, Pond SM (1990). "Purification and characterization of two human liver carboxylesterases". Int. J. Biochem. 21 (12): 1303–12. doi:10.1016/0020-711X(89)90149-3. PMID   2612723.
• Becker A, Böttcher A, Lackner KJ, et al. (1994). "Purification, cloning, and expression of a human enzyme with acyl coenzyme A: cholesterol acyltransferase activity, which is identical to liver carboxylesterase". Arterioscler. Thromb. 14 (8): 1346–55. doi: 10.1161/01.atv.14.8.1346 . PMID   8049197.
• Kroetz DL, McBride OW, Gonzalez FJ (1993). "Glycosylation-dependent activity of baculovirus-expressed human liver carboxylesterases: cDNA cloning and characterization of two highly similar enzyme forms". Biochemistry. 32 (43): 11606–17. doi:10.1021/bi00094a018. PMID   8218228.
• Shibata F, Takagi Y, Kitajima M, et al. (1993). "Molecular cloning and characterization of a human carboxylesterase gene". Genomics. 17 (1): 76–82. doi:10.1006/geno.1993.1285. PMID   8406473.
• Langmann T, Becker A, Aslanidis C, et al. (1997). "Structural organization and characterization of the promoter region of a human carboxylesterase gene". Biochim. Biophys. Acta. 1350 (1): 65–74. doi:10.1016/S0167-4781(96)00142-X. PMID   9003459.
• Brzezinski MR, Spink BJ, Dean RA, et al. (1997). "Human liver carboxylesterase hCE-1: binding specificity for cocaine, heroin, and their metabolites and analogs". Drug Metab. Dispos. 25 (9): 1089–96. PMID   9311626.
• Yan B, Matoney L, Yang D (1999). "Human carboxylesterases in term placentae: enzymatic characterization, molecular cloning and evidence for the existence of multiple forms". Placenta. 20 (7): 599–607. doi:10.1053/plac.1999.0407. PMID   10452915.
• Mori M, Hosokawa M, Ogasawara Y, et al. (1999). "cDNA cloning, characterization and stable expression of novel human brain carboxylesterase". FEBS Lett. 458 (1): 17–22. doi: 10.1016/S0014-5793(99)01111-4 . PMID   10518925. S2CID   5792413.
• Islam MR, Waheed A, Shah GN, et al. (1999). "Human egasyn binds beta-glucuronidase but neither the esterase active site of egasyn nor the C terminus of beta-glucuronidase is involved in their interaction". Arch. Biochem. Biophys. 372 (1): 53–61. doi:10.1006/abbi.1999.1449. PMID   10562416.
• Ghosh S (2001). "Cholesteryl ester hydrolase in human monocyte/macrophage: cloning, sequencing, and expression of full-length cDNA". Physiol. Genomics. 2 (1): 1–8. doi:10.1152/physiolgenomics.2000.2.1.1. PMID   11015575. S2CID   1657010.
• Ghosh S, Natarajan R (2001). "Cloning of the human cholesteryl ester hydrolase promoter: identification of functional peroxisomal proliferator-activated receptor responsive elements". Biochem. Biophys. Res. Commun. 284 (4): 1065–70. doi:10.1006/bbrc.2001.5078. PMID   11409902.
• Alam M, Ho S, Vance DE, Lehner R (2002). "Heterologous expression, purification, and characterization of human triacylglycerol hydrolase". Protein Expr. Purif. 24 (1): 33–42. doi:10.1006/prep.2001.1553. PMID   11812220.
• Satoh T, Taylor P, Bosron WF, et al. (2002). "Current progress on esterases: from molecular structure to function". Drug Metab. Dispos. 30 (5): 488–93. doi:10.1124/dmd.30.5.488. PMID   11950776. S2CID   5802731.
• Alam M, Vance DE, Lehner R (2002). "Structure-function analysis of human triacylglycerol hydrolase by site-directed mutagenesis: identification of the catalytic triad and a glycosylation site". Biochemistry. 41 (21): 6679–87. doi:10.1021/bi0255625. PMID   12022871.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Bencharit S, Morton CL, Xue Y, et al. (2003). "Structural basis of heroin and cocaine metabolism by a promiscuous human drug-processing enzyme". Nat. Struct. Biol. 10 (5): 349–56. doi:10.1038/nsb919. PMID   12679808. S2CID   1108060.
• Zhu HJ, Brinda B, Froehlich TE, Markowitz JS (2012). "A discriminative analytical method for detection of CES1A1 and CES1A2/CES1A3 genetic variants". Pharmacogenet Genomics. 22 (3): 215–8. doi:10.1097/FPC.0b013e32834f03eb. PMC   3412537 . PMID   22237548.