The Florida Central and Peninsular Railroad was the final name of a system of railroads throughout Florida, becoming part of the Seaboard Air Line Railway in 1900. The system, including some of the first railroads in Florida, stretched from Jacksonville west through Tallahassee and south to Tampa. Much of the FC&P network is still in service under the ownership of CSX Transportation.
The Riceboro Southern Railway began operations in 2004 operating on about 33 miles of track, some of which is leased from CSX Transportation. The track on which it operates is part of the ex-Seaboard Air Line route from Savannah, Georgia to Jacksonville, Florida. It runs generally from Ogeechee, Georgia, where the line splits from the CSX Savannah Subdivision, which is the former Atlantic Coast Line Railroad's Savannah-Jacksonville route, and Riceboro. It does not have any of its own locomotives; it uses Georgia Central power.
The First Coast Railroad is a class III railroad operating in Florida and Georgia, owned by Genesee & Wyoming. The name is derived from its area of operations around the First Coast of Florida.
The Florida Railroad was the first railroad to connect the east and west coasts of Florida, running from Fernandina to Cedar Key. The line later became part of the Seaboard Air Line Railroad, and, where still in use, is operated by CSX Transportation and the First Coast Railroad. The highway corridor of SR 24, US 301, and SR A1A/SR 200 closely parallels the former Florida Railroad.
The Jacksonville & Southwestern Railroad (J&SW) was a railroad that served Florida from 1899 to 1904. It was purchased by the Atlantic Coast Line Railroad in 1904. The Atlantic Coast Line would extend the line further west and it would become their Jacksonville—Wilcox Line. Some of the original right-of-way was converted to a recreational path in the rails to trails program in the 1990s.
The Callahan Subdivision is a CSX Transportation railroad subdivision within the Jacksonville Division on the former Seaboard Air Line Railroad. The sub extends northward 20 miles from Baldwin, Florida, where the Jacksonville Terminal Subdivision's S Line and Florida Gulf & Atlantic Railroad meet just north of Baldwin Yard, a classification yard. According to Jacksonville Division Timetable Number 4 published in 2005, the sub runs from milepost SM 0.18 to milepost SM 20.0, where it joins the Nahunta Subdivision, a former Atlantic Coast Line Railroad, in Callahan, Florida. The line serves as a bypass to Jacksonville.
The Brooker Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the CSX S Line at Wannee Junction in Starke to Newberry for a total of 39.6 miles. At its north end it continues south from the Wildwood Subdivision and at its south end it junctions at a wye with the Florida Northern Railroad.
The Jacksonville Terminal Subdivision is a group of railroad lines owned by CSX Transportation in and around Jacksonville, which was historically a major railroad hub. The Jacksonville Terminal Subdivision includes about 13.0 miles of track.
The Savannah Subdivision is a railroad line owned by CSX Transportation in the U.S. state of Georgia. Through the middle of Savannah, the Savannah Subdivision splits into an east route and a west route. The East Route runs from Savannah, Georgia, to Ogeechee, Georgia, for a total of 15.6 miles (25.1 km). At its north end it continues south from the Charleston Subdivision and the Columbia Subdivision both of the Florence Division and at its south end it continues south as the Nahunta Subdivision. The West Route is located in Savannah, Georgia, and is 9.7 miles (15.6 km) in length. At its north end it branches off of the Savannah Subdivision East Route and at its south end it comes back into the Savannah Subdivision East Route.
The Wildwood Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX's S Line from Baldwin south to Zephyrhills via Ocala and Wildwood for a total of 155.7 miles. The S Line is CSX's designation for the line that was the Seaboard Air Line Railroad main line from 1903 to 1967.
The Yeoman Subdivision is a railroad line owned by CSX Transportation in Florida. It runs along CSX’s S Line from Zephyrhills south to just east of Tampa via Plant City for a total of 31.2 miles.
The Charleston Subdivision is a railroad territory owned by CSX Transportation in the U.S. states of South Carolina and Georgia. The line from Florence, South Carolina, to Savannah, Georgia, for a total of 195.8 miles. At its north end it continues south from the South End Subdivision and at its south end it continues south as the Savannah Subdivision of the Jacksonville Division.
The Columbia Subdivision is a railroad line owned by CSX Transportation in South Carolina and Georgia. The line runs along CSX's S Line from Columbia, South Carolina, to Savannah, Georgia, for a total of 137.9 miles (221.9 km). At its north end it continues south from the Hamlet Subdivision and at its south end it continues south as the Savannah Subdivision of the Jacksonville Division.
The Atlantic Coast Line Railroad's Perry Cutoff was a historic rail line in northern Florida running from Monticello southeast to Perry. The line provided a shortcut through the Big Bend of Florida for rail traffic running between the Midwest and the Tampa Bay area by providing a more direct route and a bypass for the busy rail hub in Jacksonville. It was completed in 1928 to reduce travel times for its passenger trains to the west coast, or Gulf Coast, of Florida during the Florida land boom of the 1920s.
The Live Oak, Tampa and Charlotte Harbor Railroad was a historic railroad in Florida chartered by railroad tycoon Henry B. Plant. It was built as an extension of Plant's Live Oak and Rowlands Bluff Railroad. Together, the two lines ran from Live Oak, Florida to Gainesville via High Springs. The lines were completed in 1884.
The Gross Cutoff was a rail line built by the Seaboard Air Line Railroad in northern Florida. It ran from the Seaboard Air Line Railroad’s main line at a point known as Gross to Callahan connecting two pre-existing tracks.
The Folkston Cutoff is a railroad line in southern Georgia. It runs from Jesup southwest to Folkston, a distance of 54 miles. It was built in 1901 by the Plant System to allow trains from the northeast to Florida to bypass their busy terminal in Waycross.
The Atlantic Coast Line Railroad's DuPont—Lakeland Line was a historic rail line in southern Georgia and the northern west coast of Florida. On employee timetables, the line was actually divided into the DuPont—High Springs Line and the High Springs—Lakeland Line. The line was primarily used for freight, though some passenger services ran on parts of it in Florida. While parts of the line were built as early as 1863, the full line was not complete until 1913. Parts of the line in Florida are still active today.
The Seaboard Air Line Railroad’s Main Line was the backbone of the Seaboard Air Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles. Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida. While some segments of the line have been abandoned as of 2023, most of the line is still in service and is owned by the Seaboard Air Line's successor, CSX Transportation as their S-Line.
