Bismuth sulfite agar

Last updated
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). Agarplates.jpg
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars).

Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. BLBG and bismuth stop gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.

Bismuth sulfite agar typically contains (w/v): [1]

1.6% bismuth sulfite Bi2(SO3)3
1.0% pancreatic digest of casein
1.0% pancreatic digest of animal tissue
1.0% beef extract
1.0% glucose
0.8% dibasic sodium phosphate
0.06% ferrous sulfate • 7 water
pH adjusted to 7.7 at 25 °C

This medium is boiled for sterility, not autoclaved.

Related Research Articles

Pancreas An organ of the digestive system and endocrine system of vertebrates

The pancreas is an organ of the digestive system and endocrine system of vertebrates. In humans, it is located in the abdomen behind the stomach and functions as a gland. The pancreas has both an endocrine and a digestive exocrine function. As an endocrine gland, it functions mostly to regulate blood sugar levels, secreting the hormones insulin, glucagon, somatostatin, and pancreatic polypeptide. As a part of the digestive system, it functions as an exocrine gland secreting pancreatic juice into the duodenum through the pancreatic duct. This juice contains bicarbonate, which neutralizes acid entering the duodenum from the stomach; and digestive enzymes, which break down carbohydrates, proteins, and fats in food entering the duodenum from the stomach.

Iron(II) sulfate

Iron(II) sulfate (British English: iron(II) sulphate) or ferrous sulfate denotes a range of salts with the formula FeSO4·xH2O. These compounds exist most commonly as the heptahydrate (x = 7) but are known for several values of x. The hydrated form is used medically to treat iron deficiency, and also for industrial applications. Known since ancient times as copperas and as green vitriol (vitriol is an archaic name for sulfate), the blue-green heptahydrate (hydrate with 7 molecules of water) is the most common form of this material. All the iron(II) sulfates dissolve in water to give the same aquo complex [Fe(H2O)6]2+, which has octahedral molecular geometry and is paramagnetic. The name copperas dates from times when the copper(II) sulfate was known as blue copperas, and perhaps in analogy, iron(II) and zinc sulfate were known respectively as green and white copperas.

Sulfite

Sulfites or sulphites are compounds that contain the sulfite ion, SO2−
3
. The sulfite ion is the conjugate base of bisulfite. Although its acid is elusive, its salts are widely used.

Sulfate-reducing microorganism

Sulfate-reducing microorganisms (SRM) or sulfate-reducing prokaryotes (SRP) are a group composed of sulfate-reducing bacteria (SRB) and sulfate-reducing archaea (SRA), both of which can perform anaerobic respiration utilizing sulfate (SO42–) as terminal electron acceptor, reducing it to hydrogen sulfide (H2S). Therefore, these sulfidogenic microorganisms "breathe" sulfate rather than molecular oxygen (O2), which is the terminal electron acceptor reduced to water (H2O) in aerobic respiration.

Growth medium Solid, liquid or gel used to grow microorganisms or cells

A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation, or small plants like the moss Physcomitrella patens. Different types of media are used for growing different types of cells.

Iron(II) sulfide

Iron(II) sulfide or ferrous sulfide is one of a family chemical compounds and minerals with the approximate formula FeS. Iron sulfides are often iron-deficient non-stoichiometric. All are black, water-insoluble solids.

Sulfur assimilation

Sulfur is an essential element for growth and physiological functioning of plants. However, its content strongly varies between plant species and it ranges from 0.1 to 6% of the plants' dry weight.

Trypticase soy agar or tryptone soya agar (TSA) and Trypticasesoy broth or tryptone soya broth (TSB) with agar are growth media for the culturing of bacteria. They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow. They are used for a wide range of applications, including culture storage, enumeration of cells (counting), isolation of pure cultures, or simply general culture.

The sulfite process produces wood pulp that is almost pure cellulose fibers by treating wood chips with solutions of sulfite and bisulfite ions. These chemicals cleave the bonds between the cellulose and lignin components of the lignocellulose. A variety of sulfite/bisulfite salts are used, including sodium (Na+), calcium (Ca2+), potassium (K+), magnesium (Mg2+), and ammonium (NH4+). The lignin is converted to lignosulfonates, which are soluble and can be separated from the cellulose fibers. For the production of cellulose, the sulfite process competes with the Kraft process, which produces stronger fibers and is less environmentally costly.

De Man, Rogosa and Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors. It contains sodium acetate, which suppresses the growth of many competing bacteria. This medium has a clear brown colour.

In enzymology, a sulfite dehydrogenase (EC 1.8.2.1) is an enzyme that catalyzes the chemical reaction

TSI slant

The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. It is often used to differentiate enteric bacteria including Salmonella and Shigella.

Calcium sulfite

Calcium sulfite, or calcium sulphite, is a chemical compound, the calcium salt of sulfite with the formula CaSO3·x(H2O). Two crystalline forms are known, the hemihydrate and the tetrahydrate, respectively CaSO3·½(H2O) and CaSO3·4(H2O). All forms are white solids. It is most notable as the product of flue-gas desulfurization.

Sulfur is metabolized by all organisms, from bacteria and archaea to plants and animals. Sulfur is reduced or oxidized by organisms in a variety of forms. The element is present in proteins, sulfate esters of polysaccharides, steroids, phenols, and sulfur-containing coenzymes.

Cystine tryptic agar (CTA), also known as cystine trypticase agar, is a growth medium used for the identification of microorganisms.

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Tryptic soy broth or Trypticase soy broth is used in microbiology laboratories as a culture broth to grow aerobic bacteria. It is a complex, general purpose medium that is routinely used to grow certain pathogenic bacteria, which tend to have high nutritional requirements. Its agar counterpart is tryptic soy agar (TSA). One of the components of Tryptic soy broth is Phytone, which is an enzymatic digest of soybean meal.

Lysine iron agar or LIA is a differential media used to distinguish bacteria that are able to decarboxylate lysine and/or produce hydrogen sulfide from those that cannot. This test is particularly useful for distinguishing different Gram-negative bacilli—especially among the Enterobacteriaceae.

This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products. It was originally called M16 medium, but in 1975 Terzaghi and Sandine added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many Lactobacillus species.

A sulfite sulfate is a chemical compound that contains both sulfite and sulfate anions [SO3]2− [SO4]2−. These compounds were discovered in the 1980s as calcium and rare earth element salts. Minerals in this class were later discovered. Minerals may have sulfite as an essential component, or have it substituted for another anion as in alloriite. The related ions [O3SOSO2]2− and [(O2SO)2SO2]2− may be produced in a reaction between sulfur dioxide and sulfate and exist in the solid form as tetramethyl ammonium salts. They have a significant partial pressure of sulfur dioxide.

References

  1. Atlas, R.M. (2004). Handbook of Microbiological Media . London: CRC Press. pp.  68. ISBN   0-8493-1818-1.