| Tournament details | |
|---|---|
| Country |  Ukraine |
| Final positions | |
| Champions | FC Luzhany |
| Runner-up | Krystal Parkhomivka |
The 2000 Ukrainian Amateur Cup was the fifth annual season of Ukraine's football knockout competition for amateur football teams. The competition started on 2 July 2000 and concluded on 15 October 2000.
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Notes:
Five regions that were represented last season, chose not to participate in the competition among which are such oblasts Ivano-Frankivsk, Odesa, Poltava, Sumy, and Ternopil.
| Berdychiv | +:- | Yavir Tsuman | cancelled |
| Kolos Bobrovytsia | +:- | Kolos Chornobai | cancelled |
| Dynamo Stakhanov | 0:3 | Krystal Parkhomivka | 0:3 and 0:0 |
| Sokil Zolochiv | 3:3 | Perechyn | 3:1 and 0:2 |
| Kolos Bobrovytsia | -:+ | Krystal Parkhomivka | 1:3 and canc. |
| Borysfen-2 Shchaslyve | -:+ | Dnipro Kyiv | 0:1 and forfeit |
| Berdychiv | 3:3 | Tovtry Chemerivtsi | 2:0 and 1:3 |
| Ikar-MAKBO-94 Kirovohrad | -:+ | HPZ Varva | 0:2 and canc. |
| Perechyn | 1:1, pen. 5:6 | Luzhany | 1:0 and 0:1 |
| Berdychiv | 3:4 | Dnipro Kyiv | 2:1 and 1:3 |
| Monolit Kostiantynivka | -:+ | Krystal Parkhomivka | 0:3 and forfeit |
| Luzhany | 1:0 | Dnipro Kyiv | 0:0 and 1:0 |
| HPZ Varva | 1:3 | Krystal Parkhomivka | 1:3 and 0:0 |
| Luzhany | 9:5 | Krystal Parkhomivka | 5:1 and 4:4 |
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