| Doubles | |
|---|---|
| 2016 Charlottesville Men's Pro Challenger | |
| Champions |  Brian Baker  Sam Groth |
| Runners-up |  Brydan Klein  Ruan Roelofse |
| Final score | 6–3, 6–3 |
Chase Buchanan and Tennys Sandgren were the defending champions but only Sandgren chose to defend his title, partnering Austin Krajicek. Sandgren lost in the quarterfinals to Brian Baker and Sam Groth.
Baker and Groth won the title after defeating Brydan Klein and Ruan Roelofse 6–3, 6–3 in the final.
Brian Baker / Sam Groth (champions)
Peter Polansky / Adil Shamasdin (first round) Eric Quigley / Max Schnur (first round) Jarryd Chaplin / 
Ben McLachlan (first round)| First round | Quarterfinals | Semifinals | Final | ||||||||||||||||||||||||
| 1 | B Baker S Groth | 711 | 6 | ||||||||||||||||||||||||
| B Fratangelo D Kudla | 69 | 2 | 1 | B Baker S Groth | 6 | 6 | |||||||||||||||||||||
| A Krajicek T Sandgren | 4 | 79 | [10] | A Krajicek T Sandgren | 4 | 4 | |||||||||||||||||||||
 R Bemelmans J De Loore | 6 | 67 | [8] | 1 | B Baker S Groth | 6 | 77 | ||||||||||||||||||||
| 3 | E Quigley M Schnur | 3 | 2 |  M Lībietis D Novikov | 3 | 64 | |||||||||||||||||||||
| M Lībietis D Novikov | 6 | 6 | M Lībietis D Novikov | 6 | 6 | ||||||||||||||||||||||
| WC | Jared Hiltzik R Williams | 4 | 65 | L Bambridge L Broady | 1 | 4 | |||||||||||||||||||||
| L Bambridge L Broady | 6 | 77 | 1 | B Baker S Groth | 6 | 6 | |||||||||||||||||||||
 D O'Hare J Salisbury | 6 | 6 | B Klein R Roelofse | 3 | 3 | ||||||||||||||||||||||
| WC |  A Ritschard Henrik Wiersholm | 4 | 3 | D O'Hare J Salisbury | 3 | 4 | |||||||||||||||||||||
| B Klein R Roelofse | 77 | 64 | [10] | B Klein R Roelofse | 6 | 6 | |||||||||||||||||||||
| 4 | Jarryd Chaplin B McLachlan | 65 | 77 | [8] | B Klein R Roelofse | 6 | 77 | ||||||||||||||||||||
| S Bangoura M McDonald | 6 | 7 | S Bangoura M McDonald | 3 | 64 | ||||||||||||||||||||||
| WC | Luca Corinteli F Tiafoe | 1 | 5 | S Bangoura M McDonald | 6 | 6 | |||||||||||||||||||||
| Q |  Dragoș Constantin Ignat T Vorster | 6 | 3 | [10] | Q | DC Ignat T Vorster | 4 | 2 | |||||||||||||||||||
| 2 | P Polansky A Shamasdin | 3 | 6 | [8] | |||||||||||||||||||||||
Marin Čilić was the defending champion, but was defeated 7–5, 5–7, 3–6 in the final by Andy Murray. Winning in the final meant Murray became the first British player to win three titles at the Queen's Club since Arthur Lowe in the 1920s.
Tennys Sandgren and Rhyne Williams were the defending champions, but they lost in the first round.
Tim Smyczek was the defending champion but lost to Erik Crepaldi in the first round.
Unseeded American Tennys Sandgren won the title defeating Australian 7th seed Samuel Groth 3–6, 6–3, 7–6(7–5).
Samuel Groth and Toshihide Matsui were the defending champions but decided not to participate.
Mikhail Elgin and Denis Istomin were the defending champions, but Istomin chose not to participate this year. Elgin played alongside Sergey Betov and lost in the first round to Sam Groth and Chris Guccione.
František Čermák and Jiří Veselý won the title, defeating Sam Groth and Chris Guccione in the final, 7–6(7–2), 7–5.
Sam Groth and Chris Guccione were the defending champions, but lost in the quarterfinals to Wesley Koolhof and Matwé Middelkoop.
Édouard Roger-Vasselin and Radek Štěpánek won the title, defeating Mate Pavić and Michael Venus in the final, 7–5, 6–3.
This was the first edition of the tournament.
Carsten Ball and Dustin Brown were the defending champions but chose not to defend their title.
Johan Brunström and Frederik Nielsen were the defending champions but chose not to defend their title.
David O'Hare and Joe Salisbury were the defending champions but lost in the quarterfinals to Dominik Köpfer and Alex Lawson.
Dan Evans was the defending champion, but decided not to participate this year.
Malek Jaziri was the defending champion but chose not to defend his title.
Juan Mónaco was the defending champion, but lost in the first round to Dustin Brown.
Ivo Karlović was the defending champion, but lost in the quarterfinals to Peter Gojowczyk.
Jamie Murray and Bruno Soares were the defending champions, but lost in the quarterfinals to Jean-Julien Rojer and Horia Tecău.
Brian Baker and Sam Groth were the defending champions but chose not to defend their title.
Sam Groth was the defending champion but retired in the second round against Jan Choinski.
Brian Baker and Sam Groth were the defending champions but chose not to defend their title.
Steve Johnson was the defending champion and successfully defended his title, defeating Tennys Sandgren in the final, 7–6(7–2), 2–6, 6–4.
Mischa Zverev was the defending champion, but chose not to participate this year.