| Singles | |
|---|---|
| 2016 San Luis Open Challenger Tour | |
| Champions |  Peđa Krstin |
| Runners-up |  Marcelo Arévalo |
| Score | 6–4, 6–2 |
Guido Pella was the defending champion, but chose not to defend his title.
Peđa Krstin won the title, defeating Marcelo Arévalo 6–4, 6–2 in the final.
Michael Berrer (first round)
Albert Montañés (semifinals)
André Ghem (quarterfinals) Adrián Menéndez Maceiras (first round)
Matteo Donati (second round)
Mathias Bourgue (second round)
Hans Podlipnik (quarterfinals)
James McGee (first round)| Semifinals | Final | ||||||||||||
| Federico Gaio | 1 | 4 | |||||||||||
| Q | Marcelo Arévalo | 6 | 6 | ||||||||||
| Q | Marcelo Arévalo | 4 | 2 | ||||||||||
| Peđa Krstin | 6 | 6 | |||||||||||
| Peđa Krstin | 6 | 6 | |||||||||||
| 2 | Albert Montañés | 2 | 4 | ||||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 1 | M Berrer | 3 | 77 | 5 | |||||||||||||||||||||||
| F Gaio | 6 | 65 | 7 | F Gaio | 6 | 6 | |||||||||||||||||||||
| N Milojević | 6 | 6 | N Milojević | 3 | 1 | ||||||||||||||||||||||
| O Roca Batalla | 4 | 3 | F Gaio | 6 | 6 | ||||||||||||||||||||||
| WC |  M Sánchez | 3 | 2 | 7 | H Podlipnik | 4 | 1 | ||||||||||||||||||||
| J Samper Montaña | 6 | 6 | J Samper Montaña | 3 | 4 | ||||||||||||||||||||||
 J Jung | 4 | 0 | 7 | H Podlipnik | 6 | 6 | |||||||||||||||||||||
| 7 | H Podlipnik | 6 | 6 | F Gaio | 1 | 4 | |||||||||||||||||||||
| 3 | A Ghem | 7 | 77 | Q | M Arévalo | 6 | 6 | ||||||||||||||||||||
 D Novak | 5 | 62 | 3 | A Ghem | 6 | 6 | |||||||||||||||||||||
| WC | L Gómez | 6 | 6 | WC | L Gómez | 3 | 2 | ||||||||||||||||||||
 D Popko | 2 | 0 | 3 | A Ghem | 3 | 65 | |||||||||||||||||||||
| Q | M Arévalo | 6 | 6 | Q | M Arévalo | 6 | 77 | ||||||||||||||||||||
| WC | Mauricio Astorga | 2 | 3 | Q | M Arévalo | 61 | 6 | 6 | |||||||||||||||||||
 D Nguyen | 4 | 4 | 5 | M Donati | 77 | 4 | 3 | ||||||||||||||||||||
| 5 | M Donati | 6 | 6 | ||||||||||||||||||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 6 | M Bourgue | 5 | 6 | 6 | |||||||||||||||||||||||
| Alt |  N Barrientos | 7 | 2 | 0 | 6 | M Bourgue | 3 | 4 | |||||||||||||||||||
| P Krstin | 6 | 6 | P Krstin | 6 | 6 | ||||||||||||||||||||||
| WC | T Hank | 3 | 4 | P Krstin | 77 | 6 | |||||||||||||||||||||
| Q |  Robin Staněk | 6 | 6 | Q | C Zampieri | 64 | 3 | ||||||||||||||||||||
| R Ramírez Hidalgo | 3 | 4 | Q | R Staněk | 3 | 1 | |||||||||||||||||||||
| Q | C Zampieri | 6 | 1 | 7 | Q | C Zampieri | 6 | 6 | |||||||||||||||||||
| 4 | A Menéndez Maceiras | 1 | 6 | 5 | P Krstin | 6 | 6 | ||||||||||||||||||||
| 8 | J McGee | 4 | 2 | 2 | A Montañés | 2 | 4 | ||||||||||||||||||||
 M Safwat | 6 | 6 | M Safwat | 79 | 6 | ||||||||||||||||||||||
 G Lapentti | 4 | 3 | L Giustino | 67 | 4 | ||||||||||||||||||||||
| L Giustino | 6 | 6 | M Safwat | 6 | 2 | 65 | |||||||||||||||||||||
| Q |  A Velotti | 5 | 6 | 0 | 2 | A Montañés | 4 | 6 | 77 | ||||||||||||||||||
| L Djere | 7 | 3 | 6 | L Djere | 5 | 65 | |||||||||||||||||||||
| P Cachin | 7 | 3 | 65 | 2 | A Montañés | 7 | 77 | ||||||||||||||||||||
| 2 | A Montañés | 5 | 6 | 77 | |||||||||||||||||||||||
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