| Singles | |
|---|---|
| 2018 RBC Pro Challenge | |
| Champion |  Whitney Osuigwe |
| Runner-up |  Beatriz Haddad Maia |
| Score | 6–3, 6–4 |
Kristie Ahn was the defending champion, but retired in the first round against Kurumi Nara.
Whitney Osuigwe won the title, defeating Beatriz Haddad Maia in the final, 6–3, 6–4.
Belinda Bencic (semifinals) Madison Brengle (second round)
Fanny Stollár (first round) Jessica Pegula (first round) Claire Liu (second round) Nicole Gibbs (second round) Kristie Ahn (first round, retired) Christina McHale (second round)| Semifinals | Final | ||||||||||||
| 1 | Belinda Bencic | 4 | 6 | 63 | |||||||||
| JE | Whitney Osuigwe | 6 | 2 | 77 | |||||||||
| JE | Whitney Osuigwe | 6 | 6 | ||||||||||
| Beatriz Haddad Maia | 3 | 4 | |||||||||||
| Beatriz Haddad Maia | 6 | 6 | |||||||||||
| Danielle Lao | 2 | 3 | |||||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 1 | B Bencic | 6 | 6 | ||||||||||||||||||||||||
 U Eikeri | 1 | 2 | 1 | B Bencic | 6 | 7 | |||||||||||||||||||||
| V Lepchenko | 6 | 4 | 6 | V Lepchenko | 2 | 5 | |||||||||||||||||||||
 V Flink | 3 | 6 | 4 | 1 | B Bencic | 4 | 6 | 6 | |||||||||||||||||||
| Q | A Li | 2 | 5 | K Day | 6 | 2 | 2 | ||||||||||||||||||||
| K Day | 6 | 7 | K Day | 6 | 77 | ||||||||||||||||||||||
| PR | E Halbauer | 6 | 5 | 3 | 8/WC | C McHale | 4 | 63 | |||||||||||||||||||
| 8/WC | C McHale | 3 | 7 | 6 | 1 | B Bencic | 4 | 6 | 63 | ||||||||||||||||||
| 3 | F Stollár | 4 | 6 | 3 | JE | W Osuigwe | 6 | 2 | 77 | ||||||||||||||||||
| PR |  N Podoroska | 6 | 4 | 6 | PR | N Podoroska | 2 | 1 | |||||||||||||||||||
 V Cepede Royg | 0 | 2 | WC | C McNally | 6 | 6 | |||||||||||||||||||||
| WC | C McNally | 6 | 6 | WC | C McNally | 7 | 63 | 3 | |||||||||||||||||||
| JE | W Osuigwe | 6 | 5 | 6 | JE | W Osuigwe | 5 | 77 | 6 | ||||||||||||||||||
| WC | S Chang | 2 | 7 | 3 | JE | W Osuigwe | 64 | 6 | 6 | ||||||||||||||||||
 K Nara | 6 | 0 | K Nara | 77 | 3 | 2 | |||||||||||||||||||||
| 7 | K Ahn | 2 | 0r | ||||||||||||||||||||||||
| First round | Second round | Quarterfinals | Semifinals | ||||||||||||||||||||||||
| 5 | C Liu | 6 | 3 | 6 | |||||||||||||||||||||||
| Q |  B Turati | 2 | 6 | 3 | 5 | C Liu | 4 | 3 | |||||||||||||||||||
 S Karatantcheva | 6 | 6 | S Karatantcheva | 6 | 6 | ||||||||||||||||||||||
| A Kiick | 1 | 2 | S Karatantcheva | 1 | 6 | 5 | |||||||||||||||||||||
| G Brodsky | 6 | 5 | 3 | B Haddad Maia | 6 | 3 | 7 | ||||||||||||||||||||
| B Haddad Maia | 1 | 7 | 6 | B Haddad Maia | 77 | 6 | |||||||||||||||||||||
| WC | L Chirico | 77 | 6 | WC | L Chirico | 63 | 4 | ||||||||||||||||||||
| 4 | J Pegula | 64 | 0 | B Haddad Maia | 6 | 6 | |||||||||||||||||||||
| 6 | N Gibbs | 6 | 4 | 6 | D Lao | 2 | 3 | ||||||||||||||||||||
| A Muhammad | 3 | 6 | 3 | 6 | N Gibbs | 2 | 3 | ||||||||||||||||||||
| D Lao | 6 | 77 | D Lao | 6 | 6 | ||||||||||||||||||||||
| G Min | 1 | 63 | D Lao | 6 | 7 | ||||||||||||||||||||||
| Q | E Douglas | 5 | 6 | 4 | L Davis | 1 | 5 | ||||||||||||||||||||
| L Davis | 7 | 4 | 6 | L Davis | 3 | 6 | 6 | ||||||||||||||||||||
| Q | Q Gleason | 6 | 1 | 0 | 2 | M Brengle | 6 | 4 | 4 | ||||||||||||||||||
| 2 | M Brengle | 4 | 6 | 6 | |||||||||||||||||||||||
Kristie Ahn was the defending champion, but decided not to participate this year.
Rebecca Peterson was the defending champion, but chose not to participate.
Irina Khromacheva was the defending champion, but retired in the quarterfinals against Valentini Grammatikopoulou.
Karolína Plíšková was the defending champion, but chose not to participate this year.
Christina McHale was the defending champion, but lost in the semifinals to Zarina Diyas.
This was the first edition of the tournament.
Beatriz Haddad Maia was the defending champion, but chose not to participate.
Kristie Ahn was the defending champion but lost in the first round to Barbara Haas.
Vera Lapko was the defending champion, but chose to participate in Montreal.
This was the first edition of the tournament.
Sachia Vickery was the defending champion, but chose not to participate.
Garbiñe Muguruza was the defending champion and successfully defended her title, after Victoria Azarenka retired in the final with a leg injury with the score at 6–1, 3–1.
Anna Karolína Schmiedlová was the defending champion, but lost in the second round to Beatriz Haddad Maia.
Mihaela Buzărnescu was the defending champion, but lost to Daria Kasatkina in the first round.
Kiki Bertens was the defending champion and successfully defended her title, defeating Elena Rybakina in the final, 6–1, 6–3. This would be Bertens' final WTA Tour singles title before her retirement in July 2021.
Jennifer Brady defeated Jil Teichmann in the final, 6–3, 6–4 to win the singles tennis title at the 2020 Top Seed Open. Brady did not drop a set, or face a tiebreak in any set, en route to her maiden WTA Tour singles title.
Katerina Stewart was the defending champion, but lost in the first round to Ann Li.
Taylor Townsend was the defending champion, but chose not to participate as she is on maternity leave.
Lauren Davis was the defending champion having won the previous edition in 2019, but chose not to participate.
Zheng Saisai was the reigning champion but chose not to participate.