In microbiology and bacteriology, Gram stain, is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884.
Haematoxylin or hematoxylin, also called natural black 1 or C.I. 75290, is a compound extracted from heartwood of the logwood tree with a chemical formula of C
16H
14O
6. This naturally derived dye has been used as a histologic stain, ink and as a dye in the textile and leather industry. As a dye, haematoxylin has been called Palo de Campeche, logwood extract, bluewood and blackwood. In histology, haematoxylin staining is commonly followed (counterstained), with eosin, when paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology. In addition to its use in the H&E stain, haematoxylin is also a component of the Papanicolaou stain which is widely used in the study of cytology specimens.
Romanowsky staining, also known as Romanowsky–Giemsa staining, is a prototypical staining technique that was the forerunner of several distinct but similar stains widely used in hematology and cytopathology. Romanowsky-type stains are used to differentiate cells for microscopic examination in pathological specimens, especially blood and bone marrow films, and to detect parasites such as malaria within the blood. Stains that are related to or derived from the Romanowsky-type stains include Giemsa, Jenner, Wright, Field, May–Grünwald and Leishman stains. The staining technique is named after the Russian physician Dmitri Leonidovich Romanowsky (1861–1921), who was one of the first to recognize its potential for use as a blood stain.
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology, in cytology, and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues, cell populations, or organelles within individual cells.
The Zoological Journal of the Linnean Society is a monthly peer-reviewed scientific journal covering zoology published by Oxford University Press on behalf of the Linnean Society. The editor-in-chief is Maarten Christenhusz. It was established in 1856 as the Journal of the Proceedings of the Linnean Society of London. Zoology and renamed Journal of the Linnean Society of London, Zoology in 1866. It obtained its current title in 1969.
The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues. The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfite; pararosaniline and new fuchsin are not dye alternatives with comparable detection chemistry.
Developmental Biology is a peer-reviewed scientific journal. It was established in 1959 and is the official journal of the Society for Developmental Biology. It publishes research on the mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, and genetic levels. The journal is published twice a month by Elsevier.
Joachim Wilhelm Robert Feulgen was a German physician and chemist who, in 1914, developed a method for staining DNA and who also discovered plant and animal nuclear DNA congeniality.
Hematein or haematein is an oxidized derivative of haematoxylin, used in staining. Haematein should not be confused with haematin, which is a brown to black iron-containing pigment formed by decomposition of haemoglobin. In the Colour Index, haematein is called haematine.
Orcein, also archil, orchil, lacmus and C.I. Natural Red 28, are names for dyes extracted from several species of lichen, commonly known as "orchella weeds", found in various parts of the world. A major source is the archil lichen, Roccella tinctoria. Orcinol is extracted from such lichens. It is then converted to orcein by ammonia and air. In traditional dye-making methods, urine was used as the ammonia source. If the conversion is carried out in the presence of potassium carbonate, calcium hydroxide, and calcium sulfate, the result is litmus, a more complex molecule. The manufacture was described by Cocq in 1812 and in the UK in 1874. Edmund Roberts noted orchilla as a principal export of the Cape Verde islands, superior to the same kind of "moss" found in Italy or the Canary Islands, that in 1832 was yielding an annual revenue of $200,000. Commercial archil is either a powder or a paste. It is red in acidic pH and blue in alkaline pH.
Hematoxylin and eosin stain is one of the principal tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard. For example, when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E.
BioScience is a monthly peer-reviewed scientific journal that is published by Oxford University Press on behalf of the American Institute of Biological Sciences. It was established in 1964 and was preceded by the AIBS Bulletin (1951–1963).
Neurology is a weekly peer-reviewed medical journal covering research in neurology. It is published by Wolters Kluwer on behalf of the American Academy of Neurology, of which it is the official journal. It has been edited since April 2020 by José G. Merino.
Biebrich scarlet is a molecule used in Lillie's trichrome.
Behavior Genetics is a peer-reviewed scientific journal published monthly by Springer Science+Business Media that is covering "research in the inheritance of behavior". It is the official journal of the Behavior Genetics Association. The journal was established in 1971 with Steven G. Vandenberg as its founding editor-in-chief. The abstracts of the annual meetings are printed in the journal. Each year, the editorial board chooses a particularly meritorious paper in the previous year's volume of the journal for the Fulker Award, acknowledged by "$1000 and a good bottle of wine" as well as a citation made in the journal. This award was created in the honor of David Fulker, a past president of the Behavior Genetics Association (1982) and former editor-in-chief of the journal.
The Journal of Histochemistry and Cytochemistry is a peer-reviewed scientific journal of cell biology established in 1953. It covers research in the structure and function of cells, tissues, and organs as well as components of development, differentiation, and disease, as well as microscopy and imaging techniques. The journal is the official publication of The Histochemical Society and is published by SAGE Publications. The editor-in-chief as of January 1, 2016 is Stephen M. Hewitt of the National Cancer Institute, National Institutes of Health, in Bethesda Maryland, USA. The immediate past editor-in-chief is John R. Couchman. The journal is published online and in print monthly. Journal content is available for free after twelve months.
The Biological Stain Commission (BSC) is an organization that provides third-party testing and certification of dyes and a few other compounds that are used to enhance contrast in specimens examined in biological and medical laboratories.
Animal Welfare is a quarterly, peer-reviewed scientific journal covering studies on the welfare of animals, whether in captivity or in the wild. Its scope includes animal welfare science, animal cognition, ethology, behavioural ecology, evolution of behaviour, sociobiology, behavioural physiology, population biology, neurophysiology and abnormal behaviour. It was established in 1992 and is published by the Universities Federation for Animal Welfare. The editor-in-chief is James K. Kirkwood.
Stains-all is a carbocyanine dye, which stains anionic proteins, nucleic acids, anionic polysaccharides and other anionic molecules.
Harold Joel Conn was an American agricultural bacteriologist, known for his work on soil microbiology and bacterial staining techniques. He was one of the founders of the Biological Stain Commission and also founded their journal, Stain Technology. He served as president of the Society of American Bacteriologists in 1948.
