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A laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation. The word "laser" is an acronym for "light amplification by stimulated emission of radiation". The first laser was built in 1960 by Theodore H. Maiman at Hughes Research Laboratories, based on theoretical work by Charles Hard Townes and Arthur Leonard Schawlow.
Computational biology involves the development and application of data-analytical and theoretical methods, mathematical modelling and computational simulation techniques to the study of biological, ecological, behavioral, and social systems. The field is broadly defined and includes foundations in biology, applied mathematics, statistics, biochemistry, chemistry, biophysics, molecular biology, genetics, genomics, computer science, ecology, and evolution, but is most commonly thought of as the intersection of computer science, biology, and big data.
Cytogenetics is essentially a branch of genetics, but is also a part of cell biology/cytology, that is concerned with how the chromosomes relate to cell behaviour, particularly to their behaviour during mitosis and meiosis. Techniques used include karyotyping, analysis of G-banded chromosomes, other cytogenetic banding techniques, as well as molecular cytogenetics such as fluorescent in situ hybridization (FISH) and comparative genomic hybridization (CGH).
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.
Ablation is removal or destruction of something from an object by vaporization, chipping, erosive processes or by other means. Examples of ablative materials are described below, and include spacecraft material for ascent and atmospheric reentry, ice and snow in glaciology, biological tissues in medicine and passive fire protection materials.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells. In animal cells, transfection is the preferred term as transformation is also used to refer to progression to a cancerous state (carcinogenesis) in these cells. Transduction is often used to describe virus-mediated gene transfer into eukaryotic cells.
Histopathology refers to the microscopic examination of tissue in order to study the manifestations of disease. Specifically, in clinical medicine, histopathology refers to the examination of a biopsy or surgical specimen by a pathologist, after the specimen has been processed and histological sections have been placed onto glass slides. In contrast, cytopathology examines free cells or tissue micro-fragments.
A diamond anvil cell (DAC) is a high-pressure device used in geology, engineering, and materials science experiments. It enables the compression of a small (sub-millimeter-sized) piece of material to extreme pressures, typically up to around 100–200 gigapascals, although it is possible to achieve pressures up to 770 gigapascals.
The Gravity Recovery and Climate Experiment (GRACE) was a joint mission of NASA and the German Aerospace Center (DLR). Twin satellites took detailed measurements of Earth's gravity field anomalies from its launch in March 2002 to the end of its science mission in October 2017. The GRACE Follow-On (GRACE-FO) is a continuation of the mission on near-identical hardware, launched in May 2018.
A microtome is a cutting tool used to produce extremely thin slices of material known as sections. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light or electron radiation.
Microdissection refers to a variety of techniques where a microscope is used to assist in dissection.
Laser capture microdissection (LCM), also called microdissection, laser microdissection (LMD), or laser-assisted microdissection, is a method for isolating specific cells of interest from microscopic regions of tissue/cells/organisms.
Chromosome microdissection is a technique that physically removes a large section of DNA from a complete chromosome. The smallest portion of DNA that can be isolated using this method comprises 10 million base pairs - hundreds or thousands of individual genes.
Neuromorphology is the study of nervous system form, shape, and structure. The study involves looking at a particular part of the nervous system from a molecular and cellular level and connecting it to a physiological and anatomical point of view. The field also explores the communications and interactions within and between each specialized section of the nervous system. Morphology is distinct from morphogenesis. Morphology is the study of the shape and structure of biological organisms, while morphogenesis is the study of the biological development of the shape and structure of organisms. Therefore, neuromorphology focuses on the specifics of the structure of the nervous system and not the process by which the structure was developed. Neuromorphology and morphogenesis, while two different entities, are nonetheless closely linked.
The proportionator is the most efficient unbiased stereological method used to estimate population size in samples.
A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available.
Microfluidic whole genome haplotyping is a technique for the physical separation of individual chromosomes from a metaphase cell followed by direct resolution of the haplotype for each allele.
ION LMD system is one of the laser microdissection systems and a name of device that follows Gravity-Assisted Microdissection method, also known as GAM method. This non-contact laser microdissection system makes cell isolation for further genetic analysis possible. It is the first developed laser microdissection system in Asia.
Spatial transcriptomics is an overarching term for a range of methods designed for assigning cell types to their locations in the histological sections. This method can also be used to determine subcellular localization of mRNA molecules. The term is a variation of Spatial Genomics, first described by Doyle, et al., in 2000 and then expanded upon by Ståhl et. al. in a technique developed in 2016, which has since undergone a variety of improvements and modifications.
Ranjith Ramasamy is Director of the Reproductive Urology Fellowship program at the University of Miami's Miller School of Medicine.