Holography is a technique that enables a wavefront to be recorded and later reconstructed. It is best known as a method of generating three-dimensional images, and has a wide range of other uses, including data storage, microscopy, and interferometry. In principle, it is possible to make a hologram for any type of wave.
An optical neural network is a physical implementation of an artificial neural network with optical components. Early optical neural networks used a photorefractive Volume hologram to interconnect arrays of input neurons to arrays of output with synaptic weights in proportion to the multiplexed hologram's strength. Volume holograms were further multiplexed using spectral hole burning to add one dimension of wavelength to space to achieve four dimensional interconnects of two dimensional arrays of neural inputs and outputs. This research led to extensive research on alternative methods using the strength of the optical interconnect for implementing neuronal communications.
Holonomic brain theory is a branch of neuroscience investigating the idea that human consciousness is formed by quantum effects in or between brain cells. Holonomic refers to representations in a Hilbert phase space defined by both spectral and space-time coordinates. Holonomic brain theory is opposed by traditional neuroscience, which investigates the brain's behavior by looking at patterns of neurons and the surrounding chemistry.
DNA origami is the nanoscale folding of DNA to create arbitrary two- and three-dimensional shapes at the nanoscale. The specificity of the interactions between complementary base pairs make DNA a useful construction material, through design of its base sequences. DNA is a well-understood material that is suitable for creating scaffolds that hold other molecules in place or to create structures all on its own.
Acoustic levitation is a method for suspending matter in air against gravity using acoustic radiation pressure from high intensity sound waves.
Acoustic radiation pressure is the apparent pressure difference between the average pressure at a surface moving with the displacement of the wave propagation and the pressure that would have existed in the fluid of the same mean density when at rest. Numerous authors make a distinction between the phenomena of Rayleigh radiation pressure and Langevin radiation pressure.
Computer-generated holography (CGH) is a technique that uses computer algorithms to generate holograms. It involves generating holographic interference patterns. A computer-generated hologram can be displayed on a dynamic holographic display, or it can be printed onto a mask or film using lithography. When a hologram is printed onto a mask or film, it is then illuminated by a coherent light source to display the holographic images.
Acoustic radiation force (ARF) is a physical phenomenon resulting from the interaction of an acoustic wave with an obstacle placed along its path. Generally, the force exerted on the obstacle is evaluated by integrating the acoustic radiation pressure over its time-varying surface.
Multiphoton lithography is similar to standard photolithography techniques; structuring is accomplished by illuminating negative-tone or positive-tone photoresists via light of a well-defined wavelength. The main difference is the avoidance of photomasks. Instead, two-photon absorption is utilized to induce a change in the solubility of the resist for appropriate developers.
Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The image forming lens in traditional microscopy is thus replaced by a computer algorithm. Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. Common to all methods is the use of a reference wave front to obtain amplitude (intensity) and phase information. The information is recorded on a digital image sensor or by a photodetector from which an image of the object is created (reconstructed) by a computer. In traditional microscopy, which do not use a reference wave front, only intensity information is recorded and essential information about the object is lost.
Three dimensional (3D) bioprinting is the use of 3D printing–like techniques to combine cells, growth factors, bio-inks, and biomaterials to fabricate functional structures that were traditionally used for tissue engineering applications but in recent times have seen increased interest in other applications such as biosensing, and environmental remediation. Generally, 3D bioprinting uses a layer-by-layer method to deposit materials known as bio-inks to create tissue-like structures that are later used in various medical and tissue engineering fields. 3D bioprinting covers a broad range of bioprinting techniques and biomaterials. Currently, bioprinting can be used to print tissue and organ models to help research drugs and potential treatments. Nonetheless, translation of bioprinted living cellular constructs into clinical application is met with several issues due to the complexity and cell number necessary to create functional organs. However, innovations span from bioprinting of extracellular matrix to mixing cells with hydrogels deposited layer by layer to produce the desired tissue. In addition, 3D bioprinting has begun to incorporate the printing of scaffolds which can be used to regenerate joints and ligaments. Apart from these, 3D bioprinting has recently been used in environmental remediation applications, including the fabrication of functional biofilms that host functional microorganisms that can facilitate pollutant removal.
Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object.
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg. Since then, several microscopy methods have been developed to study living cells in greater detail with less effort. A newer type of imaging using quantum dots have been used, as they are shown to be more stable. The development of holotomographic microscopy has disregarded phototoxicity and other staining-derived disadvantages by implementing digital staining based on cells’ refractive index.
DNA digital data storage is the process of encoding and decoding binary data to and from synthesized strands of DNA.
Acoustic tweezers are a set of tools that use sound waves to manipulate the position and movement of very small objects. Strictly speaking, only a single-beam based configuration can be called acoustical tweezers. However, the broad concept of acoustical tweezers involves two configurations of beams: single beam and standing waves. The technology works by controlling the position of acoustic pressure nodes that draw objects to specific locations of a standing acoustic field. The target object must be considerably smaller than the wavelength of sound used, and the technology is typically used to manipulate microscopic particles.
Holotomography (HT) is a laser technique to measure the three-dimensional refractive index (RI) tomogram of a microscopic sample such as biological cells and tissues. Because the RI can serve as an intrinsic imaging contrast for transparent or phase objects, measurements of RI tomograms can provide label-free quantitative imaging of microscopic phase objects. In order to measure 3-D RI tomogram of samples, HT employs the principle of holographic imaging and inverse scattering. Typically, multiple 2D holographic images of a sample are measured at various illumination angles, employing the principle of interferometric imaging. Then, a 3D RI tomogram of the sample is reconstructed from these multiple 2D holographic images by inversely solving light scattering in the sample.
Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.
Functional ultrasound imaging (fUS) is a medical ultrasound imaging technique for detecting or measuring changes in neural activities or metabolism, such as brain activity loci, typically through measuring hemodynamic changes. It is an extension of Doppler ultrasonography.
3D printing speed measures the amount of manufactured material over a given time period, where the unit of time is measured in Seconds, and the unit of manufactured material is typically measured in units of either kg, mm or cm3, depending on the type of additive manufacturing technique.
Jonathan Cooper is Professor of Engineering in the College of Science & Engineering at the University of Glasgow. Professor Cooper has held the Wolfson Chair in Bioengineering at the school since 2009.
