Light Scanning Photomacrography (LSP), also known as Scanning Light Photomacrography (SLP) or Deep-Field Photomacrography, is a photographic film technique that allows for high magnification light imaging with exceptional depth of field (DOF). This method overcomes the limitations of conventional macro photography, which typically only keeps a portion of the subject in acceptable focus at high magnifications.
The principles of LSP were first documented in the early 1960s by Dan McLachlan Jr., who highlighted its capability for extreme focal depth in microscopy [1] and in 1968 patented the process. [2]
The technique was revived and further developed in the 1980s by photographers such as Darwin Dale and Nile Root, a faculty member at the Rochester Institute of Technology. [3] In the early 1990s, William Sharp and Charles Kazilek, both researchers at Arizona State University, also published articles describing their technique and system setup for capturing SLP images. [4]
Light Scanning Photomacrography offered a powerful analog tool for high-detail imaging in the age of film photography. It provided a comprehensive depth of field, making it invaluable in scientific and biomedical photography. [5] As technology and techniques continue to evolve, LSP has been replaced by digital image focus stacking. This technique uses a collection of images captured in series at different focal depths, which are then processed using computer software to create a single image with a greater focus depth than any single image.
LSP involves the use of a thin plane of light that scans across the subject, which is mounted on a stage moving perpendicular to the film plane. The technique utilizes traditional optics and is governed by the physical laws of depth of field. By moving the subject through a narrow band of illumination, the entire subject can be recorded in sharp focus from the nearest details to the farthest ones. This analog process produces sharp and detailed images by slowly recording the image on film as the specimen passes through the sheet of light that is thinner than the effective DOF. [4]
Because the image is captured at the same relative distance from the camera lens, the resulting images are axonometric rather than perspective projection, which is what the human eye sees and is typically captured by a film camera. Because all parts of an LSP image are captured at the same distance from the lens, relative measurements can be taken from an LSP photograph and can be used for comparison. [6]
A typical LSP setup includes:
In 1991, Sharp and Kazilek described their SLP system that used three Kodak Ektagraphic slide projectors with zoom lenses to create a thin plane of light. The projectors each had a slide mount with two razor blades placed edge-to-edge to create a thin slit for the light to pass through. The image was captured using a Nikon FE-2 SLR camera mounted above the specimen. Kodachrome 25 slide film was used to record the image and to minimize film grain size and maximize image sharpness [7]
A commercial SLP instrument was produced by the Irvine Optical Corp. Their DYNAPHOT system was based on a photomacroscope and could capture images on 4x5 film. The instrument came with two or three illumination sources and a motorized specimen stage. The system advertised a 2X – 40X magnification range and the ability to capture images in black and white and color. [4] Other systems have been developed by Nile Root and Theodore Clarke and reported higher magnification (up to 100X). [3]
LSP was particularly useful in biomedical photography, where it was used to document magnified subjects with increased depth of field over traditional macro and micro photography. It has been employed to capture detailed images of biological specimens, such as imaging small insects and their parts. SLP has been used to document shell collections for scientific documentation and research. Other applications include forensic science, mineralogy, and the imaging of fractured surfaces and parts [8] [9] [7] [10]
Enthusiasts and researchers have contributed to the development and accessibility of LSP by creating and sharing DIY guides. These contributions have enabled others to build their own LSP systems using readily available materials and components. Nile Root's publications provide detailed instructions and recommendations for constructing an LSP setup. These DIY systems have allowed a wider audience to explore and utilize the benefits of LSP imaging in various fields. [6] [10] [4]
The depth of field (DOF) is the distance between the nearest and the farthest objects that are in acceptably sharp focus in an image captured with a camera. See also the closely related depth of focus.
A single-lens reflex camera (SLR) is a camera that typically uses a mirror and prism system that permits the photographer to view through the lens and see exactly what will be captured. With twin lens reflex and rangefinder cameras, the viewed image could be significantly different from the final image. When the shutter button is pressed on most SLRs, the mirror flips out of the light path, allowing light to pass through to the light receptor and the image to be captured.
In optics, the aperture of an optical system is a hole or an opening that primarily limits light propagated through the system. More specifically, the entrance pupil as the front side image of the aperture and focal length of an optical system determine the cone angle of a bundle of rays that comes to a focus in the image plane.
A view camera is a large-format camera in which the lens forms an inverted image on a ground-glass screen directly at the film plane. The image is viewed, composed, and focused, then the glass screen is replaced with the film to expose exactly the same image seen on the screen.
The focal length of an optical system is a measure of how strongly the system converges or diverges light; it is the inverse of the system's optical power. A positive focal length indicates that a system converges light, while a negative focal length indicates that the system diverges light. A system with a shorter focal length bends the rays more sharply, bringing them to a focus in a shorter distance or diverging them more quickly. For the special case of a thin lens in air, a positive focal length is the distance over which initially collimated (parallel) rays are brought to a focus, or alternatively a negative focal length indicates how far in front of the lens a point source must be located to form a collimated beam. For more general optical systems, the focal length has no intuitive meaning; it is simply the inverse of the system's optical power.
In photography, angle of view (AOV) describes the angular extent of a given scene that is imaged by a camera. It is used interchangeably with the more general term field of view.
A camera lens is an optical lens or assembly of lenses used in conjunction with a camera body and mechanism to make images of objects either on photographic film or on other media capable of storing an image chemically or electronically.
Underwater photography is the process of taking photographs while under water. It is usually done while scuba diving, but can be done while diving on surface supply, snorkeling, swimming, from a submersible or remotely operated underwater vehicle, or from automated cameras lowered from the surface.
Large format photography refers to any imaging format of 9 cm × 12 cm or larger. Large format is larger than "medium format", the 6 cm × 6 cm or 6 cm × 9 cm size of Hasselblad, Mamiya, Rollei, Kowa, and Pentax cameras, and much larger than the 24 mm × 36 mm frame of 35 mm format.
In photography, bracketing is the general technique of taking several shots of the same subject using different camera settings, typically with the aim of combining the images in postprocessing. Bracketing is useful and often recommended in situations that make it difficult to obtain a satisfactory image with a single shot, especially when a small variation in exposure parameters has a comparatively large effect on the resulting image. Given the time it takes to accomplish multiple shots, it is typically, but not always, used for static subjects. Autobracketing is a feature of many modern cameras. When set, it will automatically take several bracketed shots, rather than the photographer altering the settings by hand between each shot.
Macro photography is extreme close-up photography, usually of very small subjects and living organisms like insects, in which the size of the subject in the photograph is greater than life-size . By the original definition, a macro photograph is one in which the size of the subject on the negative or image sensor is life-size or greater. In some senses, however, it refers to a finished photograph of a subject that is greater than life-size.
Depth of focus is a lens optics concept that measures the tolerance of placement of the image plane in relation to the lens. In a camera, depth of focus indicates the tolerance of the film's displacement within the camera and is therefore sometimes referred to as "lens-to-film tolerance".
In digital photography, the crop factor, format factor, or focal length multiplier of an image sensor format is the ratio of the dimensions of a camera's imaging area compared to a reference format; most often, this term is applied to digital cameras, relative to 35 mm film format as a reference. In the case of digital cameras, the imaging device would be a digital image sensor. The most commonly used definition of crop factor is the ratio of a 35 mm frame's diagonal (43.3 mm) to the diagonal of the image sensor in question; that is, . Given the same 3:2 aspect ratio as 35mm's 36 mm × 24 mm area, this is equivalent to the ratio of heights or ratio of widths; the ratio of sensor areas is the square of the crop factor.
A depth-of-field adapter is used to achieve shallow depth of field on a video camera whose fixed lens or interchangeable lens selection is limited or economically prohibitive at providing such an effect. A DOF adapter could theoretically be used on a multitude of platforms, although it is most useful on prosumer digital camcorders where high resolution is a capability but the sensor size is still small enough to elicit use of the adapter. The term 35mm adapter is common, since most designs use a focusing screen the size of a 35mm film frame and interface with lenses designed for 35mm cameras. The use of adapters has decreased largely due to the video function available on newer DSLR cameras. DOF adapters have been developed for use with smartphones, allowing DSLR lenses to be used with phone cameras.
Tilt–shift photography is the use of camera movements that change the orientation or position of the lens with respect to the film or image sensor on cameras.
Miniature faking, also known as diorama effect or diorama illusion, is a process in which a photograph of a life-size location or object is made to look like a photograph of a miniature scale model. Blurring parts of the photo simulates the shallow depth of field normally encountered in close-up photography, making the scene seem much smaller than it actually is; the blurring can be done either optically when the photograph is taken, or by digital postprocessing. Many diorama effect photographs are taken from a high angle to simulate the effect of looking down on a miniature. Tilt–shift photography is also associated with miniature faking.
Scanography, more commonly referred to as scanner photography, is the process of capturing digitized images of objects for the purpose of creating printable art using a flatbed "photo" scanner with a CCD array capturing device. Fine art scanography differs from traditional document scanning by using atypical objects, often three-dimensional, as well as from photography, due to the nature of the scanner's operation.
Tilted plane photography is a method of employing focus as a descriptive, narrative or symbolic artistic device. It is distinct from the more simple uses of selective focus which highlight or emphasise a single point in an image, create an atmospheric bokeh, or miniaturise an obliquely-viewed landscape. In this method the photographer is consciously using the camera to focus on several points in the image at once while de-focussing others, thus making conceptual connections between these points.
Focus stacking – also called focal plane merging, z-stacking, or focus blending – is a digital image processing technique which combines multiple images taken at different focus distances to give a resulting image with a greater depth of field (DOF) than any of the individual source images. Focus stacking can be used in any situation where individual images have a very shallow depth of field; macro photography and optical microscopy are two typical examples. Focus stacking can also be useful in landscape photography.
The Canon TS-E 90 mm f/2.8 is a tilt-shift, telephoto prime lens that provides the equivalent of the corresponding view camera front movements on Canon EOS camera bodies. Unlike most other EF-mount lenses, it does not provide autofocus.