This is a list of Charlotte 49ers football players in the NFL draft.
| B | Back | K | Kicker | NT | Nose tackle |
| C | Center | LB | Linebacker | FB | Fullback |
| DB | Defensive back | P | Punter | HB | Halfback |
| DE | Defensive end | QB | Quarterback | WR | Wide receiver |
| DT | Defensive tackle | RB | Running back | G | Guard |
| E | End | T | Offensive tackle | TE | Tight end |
| Year | Round | Pick | Overall | Player | Team | Position |
|---|---|---|---|---|---|---|
| 2017 | 3 | 1 | 65 | Larry Ogunjobi | Cleveland Browns | DT |
| 2019 | 3 | 18 | 82 | Nate Davis | Tennessee Titans | G |
| 2020 | 3 | 38 | 102 | Alex Highsmith | Pittsburgh Steelers | DE |
| 4 | 23 | 129 | Cameron Clark | New York Jets | G | |
| 2023 | 7 | 39 | 256 | Grant DuBose | Green Bay Packers | WR |