VirCapSeq

Last updated

VirCapSeq is a system to broadly screen for all viral infections in vertebrates including humans. [1] It was designed by W. Ian Lipkin, Thomas Briese, and Amit Kapoor at Columbia University. [2]

The researchers created a library of 2 million 50 to 100-mer oligonucleotides based on viral genome sequences described in the European Molecular Biology Laboratory database that represented the coding sequences of all known vertebrate viruses. [3] They then developed an assay whereby addition of these probes to samples allowed recovery of complete viral genomic sequences, This assay is "VirCapSeq". [3]

References

  1. ↑ Briese, Thomas; Kapoor, Amit; Mishra, Nischay; Jain, Komal; Kumar, Arvind; Jabado, Omar J.; Lipkin, W. Ian (22 September 2015). "Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis". mBio. 6 (5): e01491-15. doi: 10.1128/mBio.01491-15 . PMC   4611031 . PMID   26396248.
  2. ↑ Yong, Ed (Sep 22, 2015). "New Technique Can Cheaply and Efficiently Detect All Known Human Viruses in a Blood Sample". theatlantic.com. Retrieved 6 October 2015.
  3. 1 2 Heger, Monica (25 September 2015). "Columbia University Team Develops NGS Method to Screen for Viruses From Clinical Samples". genomeweb.com. Retrieved 7 October 2015.
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