ZebraBox

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The ZebraBox [1] is an automated analysis chamber for the non-intrusive video observation of different types of freshwater marine indicator species, such as Dania rerio and Pimephales promelas . It is a type of Larval Photomoter Response (LPR) assay, which is used to monitor the swimming behaviour of larvae. [2]

The ZebraBox contains a controlled enclosed system of 96-well plates containing a high-resolution camera fitted with an infrared light and a fixed-angle lens. [3] The lighting conditions and illumination patterns can be manually controlled for fish acclimation and the simulation of circadian rhythms. [4] The apparatus allows for the analysis of zebrafish locomotion and activity, thus it is used in the fields of drug discovery and toxicological studies. [5] [6]

References

  1. ↑ "ZebraBox". ViewPoint Life Sciences. Archived from the original on 2013-10-16. Retrieved 2013-10-16.
  2. ↑ Dasgupta S, Simonich MT, Tanguay RL (2022). "Zebrafish Behavioral Assays in Toxicology". In Zhu H, Xia M (eds.). High-Throughput Screening Assays in Toxicology. Methods in Molecular Biology. Vol. 2474. New York, NY: Springer US. pp. 109–122. doi:10.1007/978-1-0716-2213-1_11. ISBN   978-1-0716-2213-1. PMID   35294760.
  3. ↑ Lee DA, Oikonomou G, Prober DA (February 2022). "Large-scale Analysis of Sleep in Zebrafish". Bio-Protocol. 12 (3) e4313. doi:10.21769/BioProtoc.4313. PMC   8855086 . PMID   35284597.
  4. ↑ Wang W, Ma Q, Ding X, Xu Y, He M, Xu J, et al. (March 2022). "Developmental toxicity of bromoacetamide via the thyroid hormone receptors-mediated disruption of thyroid hormone homeostasis in zebrafish embryos". Ecotoxicology and Environmental Safety. 233 113334. Bibcode:2022EcoES.23313334W. doi: 10.1016/j.ecoenv.2022.113334 . PMID   35203007. S2CID   247090575.
  5. ↑ Nasri A, Lafon PA, Mezni A, Clair P, Cubedo N, Mahmoudi E, et al. (April 2022). "Developmental exposure to the A6-pesticide causes changes in tyrosine hydroxylase gene expression, neurochemistry, and locomotors behavior in larval zebrafish". Toxicology Mechanisms and Methods. 32 (8): 569–579. doi:10.1080/15376516.2022.2056100. PMID   35313786. S2CID   247599353.
  6. ↑ Liu J, Xu Y, Liao G, Tu H, Huang Y, Peng T, et al. (February 2022). "The role of ambra1 in Pb-induced developmental neurotoxicity in zebrafish". Biochemical and Biophysical Research Communications. 594: 139–145. Bibcode:2022BBRC..594..139L. doi:10.1016/j.bbrc.2021.12.084. PMID   35085890. S2CID   245496902.
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