Plot
After the title is shown, the film starts on the ocean, where an intense thunderstorm is raging. A group of red pirates are sailing on their ship, and Red Pirate Ron, who speaks in a language only understood by his parrot, Stan, warns his pirates to lower the sails. Meanwhile, Tom, who is also on the side of Red Pirate Ron, and Jerry, who is on no side at all, travel aboard the same pirate ship. Tom gets in trouble for slacking and is thrown around the ship. Tom then gets mad at Jerry, thinking this was all his fault and chases him, Jerry ends up blowing Tom up in a cannon. It is soon revealed that Ron is searching for the "Lost Treasure of the Spanish Mane" and by chance, a wave carries the map to the treasure aboard. Tom hides the map, but is warned by the ghost of the Spanish Mane's captain, Don Diego de Clippershears, that if the map is not put back into its bottle by sunset, a curse will begin. However, the bottle is swept off the ship, leaving Tom with no way to elude the curse.
At one point, Tom and Jerry escape from a giant squid knocking it out with a cannon. Tom shoots Jerry off Ron's ship, and onto another ship commanded by Ron's brother, Blue Pirate Bob, who also speaks with the help of his parrot, Betty. Jerry tells them about the map to avoid being eaten by Spike and alerts them to Ron's ship. Bob's crew manages to steal the map, but Ron retrieves it and shoots down his brother's ship. Ron's crew celebrates, but Jerry stops them by grabbing the treasure bottle from the ship, explaining the reason Tom lost the treasure bottle.
The sun sets, the ghost appears, and begins the curse as a crew of skeleton pirates are called forth. Ron's crew abandons ship, and their lifeboat lands on top of the giant squid that Tom and Jerry fought earlier. For not telling him about the curse (unaware that he can't speak), Ron makes Tom row. Eventually as they near Yo Ho Island, Tom deserts and paddles off on an oar with Jerry following him.
When Tom and Jerry arrive, they fight over who should keep the map. But when Tom falls in quicksand, Jerry saves him. The duo decide to stop fighting so they find the treasure together. After walking for a while, they meet Purple Pirate Paul, Ron and Bob's brother, and his parrot, Chuck. Paul, who leads a crew of monkeys and can actually speak in real words (whilst Chuck can not), tells him how the three used to make treasure maps when they were little aboard their mother's green pirate ship, but after they fought over the map about the Spanish Mane, their mother took all their maps, bottled them, and threw them overboard. Since Paul saw more of the map then his brothers, he took a rowboat to the island to search for the treasure and remained there for the next 40 years. Then, Ron and his crew show up and reunite with Paul, who insists on being called “Barnacle Paul” rather than Purple Pirate Paul, allowing Tom and Jerry to escape.
Tom and Jerry escape the pirates and monkeys and reach Don Diego's tomb, where the treasure is. Don Diego's spirit explains that they must defeat the tomb's guardian to enter: a giant stone chicken. They manage to defeat it and retrieve a stone egg, which is the key to unlocking the tomb's entrance, though Tom ends their partnership due to Jerry using him as live bait. Because the entrance's lock resembles a frying pan, the duo solve the puzzle by cracking open the egg and placing it on the stone pan. After maneuvering through many traps and tests (including crushing pillars and a memory game), they reach the cave that contains the treasure. Don Diego urges Jerry to take it, but this makes Jerry suspicious, sensing a trick. He is proven correct when he sees that he must deal with the treasure's guardian first. Tom rushes inside and is shocked to see the same giant squid from earlier standing guard. When it recognizes Tom, it shrieks in fear and a stalactite crushes it. Tom and Jerry retrieve some of the treasure and get out. When they exit the tomb, Ron, Paul and Bob (after dismissing their parrots) begin to fight over the treasure. Jerry seizes this opportunity to sneak it onto Bob's ship, which has been fully repaired, and hoist the anchor, causing the ship to start sailing away. Tom and Spike follow Jerry by catapulting themselves onto the ship, where Jerry gives a Spike a gold bone. The three commandeer the ship as it departs from the island, to the pirates’ horror.
In the end, Jerry becomes captain, with Spike being made to pilot the ship, and Tom being made to swab the deck (with Spike ensuring that he doesn't slack); Stan, Betty, and Chuck join Jerry's crew; and all the now stranded pirates run from the stone chicken, which somehow reassembled itself.
