ORC5

Last updated
ORC5
Identifiers
Aliases ORC5 , ORC5L, ORC5P, ORC5T, PPP1R117, origin recognition complex subunit 5
External IDs OMIM: 602331 MGI: 1347044 HomoloGene: 37636 GeneCards: ORC5
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001197292
NM_002553
NM_181747

NM_011959

RefSeq (protein)

NP_002544
NP_859531

NP_036089

Location (UCSC) Chr 7: 104.13 – 104.21 Mb Chr 5: 22.69 – 22.76 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. [5] [6] [7]

Contents

Function

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and 4L. Alternatively spliced transcript variants encoding distinct isoforms have been described. [7]

Interactions

ORC5 has been shown to interact with:

Related Research Articles

<span class="mw-page-title-main">MCM6</span>

DNA replication licensing factor MCM6 is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication.

In molecular biology, origin recognition complex (ORC) is a multi-subunit DNA binding complex that binds in all eukaryotes and archaea in an ATP-dependent manner to origins of replication. The subunits of this complex are encoded by the ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6 genes. ORC is a central component for eukaryotic DNA replication, and remains bound to chromatin at replication origins throughout the cell cycle.

<span class="mw-page-title-main">Eukaryotic DNA replication</span> DNA Replication in eukaryotic

Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.

<span class="mw-page-title-main">Minichromosome maintenance</span>

The minichromosome maintenance protein complex (MCM) is a DNA helicase essential for genomic DNA replication. Eukaryotic MCM consists of six gene products, Mcm2–7, which form a heterohexamer. As a critical protein for cell division, MCM is also the target of various checkpoint pathways, such as the S-phase entry and S-phase arrest checkpoints. Both the loading and activation of MCM helicase are strictly regulated and are coupled to cell growth cycles. Deregulation of MCM function has been linked to genomic instability and a variety of carcinomas.

<span class="mw-page-title-main">Replication protein A1</span> Protein-coding gene in the species Homo sapiens

Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.

<span class="mw-page-title-main">MCM7</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.

<span class="mw-page-title-main">MCM2</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene.

<span class="mw-page-title-main">MCM3</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene.

<span class="mw-page-title-main">CDC6</span> Protein-coding gene in the species Homo sapiens

Cell division control protein 6 homolog is a protein that in humans is encoded by the CDC6 gene.

<span class="mw-page-title-main">MCM4</span> Protein-coding gene in the species Homo sapiens

DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene.

<span class="mw-page-title-main">ORC2</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 2 is a protein that is encoded by the ORC2 (ORC2L) gene in humans.

<span class="mw-page-title-main">Cell division cycle 7-related protein kinase</span> Protein-coding gene in the species Homo sapiens

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The gene CDC7 appears to be conserved throughout eukaryotic evolution; this means that most eukaryotic cells have the Cdc7 kinase protein.

<span class="mw-page-title-main">DBF4</span> Protein-coding gene in the species Homo sapiens

Protein DBF4 homolog A is a protein that is encoded by the DBF4 gene in humans.

<span class="mw-page-title-main">ORC4</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 4 is a protein that in humans is encoded by the ORC4 (ORC4L) gene.

<span class="mw-page-title-main">ORC6</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 6 is a protein that in humans is encoded by the ORC6 (ORC6L) gene.

<span class="mw-page-title-main">MCM10</span> Protein-coding gene in the species Homo sapiens

Protein MCM10 homolog is a protein that in humans is encoded by the MCM10 gene. It is essential for activation of the Cdc45:Mcm2-7:GINS helicase, and thus required for proper DNA replication.

<span class="mw-page-title-main">CDC45-related protein</span> Protein-coding gene in the species Homo sapiens

CDC45 is a protein that in humans is encoded by the CDC45L gene.

<span class="mw-page-title-main">ORC3</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3 (ORC3L) gene.

<span class="mw-page-title-main">Cdc6</span>

Cdc6, or cell division cycle 6, is a protein in eukaryotic cells. It is mainly studied in the budding yeast Saccharomyces cerevisiae. It is an essential regulator of DNA replication and plays important roles in the activation and maintenance of the checkpoint mechanisms in the cell cycle that coordinate S phase and mitosis. It is part of the pre-replicative complex (pre-RC) and is required for loading minichromosome maintenance (MCM) proteins onto the DNA, an essential step in the initiation of DNA synthesis. In addition, it is a member of the family of AAA+ ATPases and highly related to ORC1; both are the same protein in archaea.

<span class="mw-page-title-main">ORC1</span> Protein-coding gene in the species Homo sapiens

Origin recognition complex subunit 1 is a protein that in humans is encoded by the ORC1 gene. It is closely related to CDC6, and both are the same protein in archaea.

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000164815 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000029012 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Ishiai M, Dean FB, Okumura K, Abe M, Moon KY, Amin AA, Kagotani K, Taguchi H, Murakami Y, Hanaoka F, O'Donnell M, Hurwitz J, Eki T (Dec 1997). "Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22". Genomics. 46 (2): 294–8. doi:10.1006/geno.1997.5003. PMID   9417919.
  6. Tugal T, Zou-Yang XH, Gavin K, Pappin D, Canas B, Kobayashi R, Hunt T, Stillman B (Dec 1998). "The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p". The Journal of Biological Chemistry. 273 (49): 32421–9. doi: 10.1074/jbc.273.49.32421 . PMID   9829972.
  7. 1 2 "Entrez Gene: ORC5L origin recognition complex, subunit 5-like (yeast)".
  8. 1 2 3 4 5 6 7 8 Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID   12614612.
  9. 1 2 Quintana DG, Thome KC, Hou ZH, Ligon AH, Morton CC, Dutta A (Oct 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". The Journal of Biological Chemistry. 273 (42): 27137–45. doi: 10.1074/jbc.273.42.27137 . PMID   9765232.
  10. 1 2 3 Dhar SK, Delmolino L, Dutta A (Aug 2001). "Architecture of the human origin recognition complex". The Journal of Biological Chemistry. 276 (31): 29067–71. doi: 10.1074/jbc.M103078200 . PMID   11395502.
  11. 1 2 Vashee S, Simancek P, Challberg MD, Kelly TJ (Jul 2001). "Assembly of the human origin recognition complex". The Journal of Biological Chemistry. 276 (28): 26666–73. doi: 10.1074/jbc.M102493200 . PMID   11323433.
  12. Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A (May 1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant". Neuron. 23 (1): 45–54. doi: 10.1016/s0896-6273(00)80752-7 . PMID   10402192. S2CID   781511.

Further reading