Names | |||
---|---|---|---|
Preferred IUPAC name Phenylthiourea | |||
Other names N-Phenylthiourea 1-Phenylthiourea | |||
Identifiers | |||
3D model (JSmol) | |||
ChEBI | |||
ChemSpider | |||
ECHA InfoCard | 100.002.865 | ||
MeSH | Phenylthiourea | ||
PubChem CID | |||
UNII | |||
CompTox Dashboard (EPA) | |||
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Properties | |||
C7H8N2S | |||
Molar mass | 152.22 g·mol−1 | ||
Appearance | White to slightly yellow powder | ||
Density | 1.294 g/cm3 | ||
Melting point | 145 to 150 °C (293 to 302 °F; 418 to 423 K) | ||
Soluble in boiling water | |||
Hazards | |||
GHS labelling: [1] | |||
Danger | |||
H300, H317 | |||
P280, P301+P310+P330, P302+P352 | |||
NFPA 704 (fire diamond) | |||
Lethal dose or concentration (LD, LC): | |||
LD50 (median dose) | 3 mg/kg (oral, rat) | ||
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). |
Phenylthiocarbamide (PTC), also known as phenylthiourea (PTU), is an organosulfur thiourea containing a phenyl ring.
It has the unusual property that it either tastes very bitter or is virtually tasteless, depending on the genetic makeup of the taster. The ability to taste PTC is often treated as a dominant genetic trait, although inheritance and expression of this trait are somewhat more complex. [2] [3]
PTC also inhibits melanogenesis and is used to grow transparent fish. [4]
About 70% of people can taste PTC, varying from a low of 58% for Indigenous Australians and indigenous peoples of New Guinea to 98% for indigenous peoples of the Americas. [5] One study has found that non-smokers and those not habituated to coffee or tea have a statistically higher percentage of tasting PTC than the general population. [6] [7] PTC does not occur in food, but related chemicals do, and food choice is related to a person's ability to taste PTC. [6] [8]
The tested genetic taste phenomenon of PTC was discovered in 1931 when DuPont chemist Arthur Fox accidentally released a cloud of fine crystalline PTC. A nearby colleague complained about the bitter taste, while Fox, who was closer and should have received a strong dose, tasted nothing. Fox then continued to test the taste buds of assorted family and friends, setting the groundwork for future genetic studies. The genetic penetrance was so strong that it was used in paternity tests before the advent of DNA matching. [9]
The PTC taste test has been widely used in school and college practical teaching as an example of Mendelian polymorphism in human populations. Based on a taste test, usually of a piece of paper soaked in PTC (or the less toxic propylthiouracil (PROP)), students are divided into taster and non-taster groups. By assuming that PTC tasting is determined by a dominant allele at a single autosomal gene, and that the class is an unbiased sample from a population in Hardy–Weinberg equilibrium, students then estimate allele and genotype frequencies within the larger population. While this interpretation is broadly consistent with numerous studies of this trait, it is worth noting that other genes, sex, age and environmental factors influence sensitivity to PTC. [2] [3] Also, there are several alleles segregating at the major gene determining the taste of PTC, particularly in African populations, and the common "taster" allele is incompletely dominant (homozygotes for this allele are more sensitive to PTC than are heterozygotes). [3] [10] Additionally, PTC is toxic and sensitivity to the substitute, PROP, does not show a strong association with the gene controlling ability to taste PTC. [3]
There is a large body of evidence linking the ability to taste thiourea compounds and dietary habits. Much of this work has focused on 6-propyl-2-thiouracil (PROP), a compound related to PTC that has lower toxicity. [5] A supertaster has more of an ability to taste PTC. On the other hand, heavy cigarette smokers are more likely to have high PTC and PROP thresholds (i.e. are relatively insensitive).
In 1976, an inverse relationship between taster status for PTC and for a bitter component of the fruit of the tree Antidesma bunius was discovered. [11] Research on the implications still continues.
Ability to taste PTC may be correlated with a dislike of plants in the genus Brassica , presumably due to chemical similarities. However, studies in Africa show a poor correlation between PTC tasting and dietary differences. [10]
Much of the variation in tasting of PTC is associated with polymorphism at the TAS2R38 taste receptor gene. [12] In humans, there are three SNPs (single nucleotide polymorphisms) along the gene that may render its proteins unresponsive. [13] There is conflicting evidence as to whether the inheritance of this trait is dominant or incompletely dominant. [3] Any person with a single functional copy of this gene can make the protein and is sensitive to PTC.[ citation needed ] Some studies have shown that homozygous tasters experience a more intense bitterness than people that are heterozygous; other studies have indicated that another gene may determine taste sensitivity. [2]
The frequency of PTC taster and non-taster alleles vary in different human populations. [14] The widespread occurrence of non-taster alleles at intermediate frequencies, much more common than recessive alleles conferring genetic disease, across many isolated populations, suggests that this polymorphism may have been maintained through balancing selection. [10]
Chimpanzees and orangutans also vary in their ability to taste PTC, with the proportions of tasters and non-tasters similar to that in humans. [15] The ability to taste PTC is an ancestral trait of hominids that has been independently lost in humans and chimpanzees, through distinct mutations at TAS2R38. [16]
Location | # of Participants | Non-taster % | References |
---|---|---|---|
Bosnia and Herzegovina | 7,362 | 32.02 | Hadžiselimović et al. (1982) [18] |
Croatia | 200 | 27.5 | Grünwald, Pfeifer (1962) |
Czech Republic | 785 | 32.7 | Kubičkova, Dvořaková (1968) |
Denmark | 251 | 32.7 | Harrison et al. (1977) [19] |
England | 441 | 31.5 | Harrison et al. (1977) [19] |
Hungary | 436 | 32.2 | Forai, Bankovi (1967) |
Italy | 1,031 | 29.19 | Floris et al. (1976) |
Montenegro | 256 | 28.20 | Hadžiselimović et al. (1982) [18] |
Užice, Serbia | 1,129 | 16.65 | Hadžiselimović et al. (1982) [18] |
Voivodina, Serbia | 600 | 26.3 | Božić, Gavrilović (1973) |
Russia | 486 | 36.6 | Boyd (1950) |
Slovenia | 126 | 37.3 | Brodar (1970) |
Spain | 204 | 25.6 | Harrison et al. (1977) [19] |
An allele, or allelomorph, is a variant of the sequence of nucleotides at a particular location, or locus, on a DNA molecule.
Heredity, also called inheritance or biological inheritance, is the passing on of traits from parents to their offspring; either through asexual reproduction or sexual reproduction, the offspring cells or organisms acquire the genetic information of their parents. Through heredity, variations between individuals can accumulate and cause species to evolve by natural selection. The study of heredity in biology is genetics.
In genetics, dominance is defined as the interactions between alleles at the same locus on homologous chromosomes and the associated phenotype. In the case of complete dominance, one allele in a heterozygote individual completely overrides or masks the phenotypic contribution of the other allele. The overriding allele is referred to as dominant and the masked one recessive. Complete dominance, also referred to as Mendelian inheritance, follow Mendel's laws of segregation. The first law states that each allele in a pair of genes is separated at random and have an equal probability of being transferred to the next generation, while the second law states that the distribution of allele variants is done independently of each other. However, this is not always the case as not all genes segregate independently and violations of this law are often referred to as "non-Mendelian inheritance".
Population genetics is a subfield of genetics that deals with genetic differences within and among populations, and is a part of evolutionary biology. Studies in this branch of biology examine such phenomena as adaptation, speciation, and population structure.
Human variability, or human variation, is the range of possible values for any characteristic, physical or mental, of human beings.
In genetics and bioinformatics, a single-nucleotide polymorphism is a germline substitution of a single nucleotide at a specific position in the genome that is present in a sufficiently large fraction of considered population.
Brassica oleracea is a plant species from family Brassicaceae that includes many common cultivars used as vegetables, such as cabbage, broccoli, cauliflower, kale, Brussels sprouts, collard greens, Savoy cabbage, kohlrabi, and gai lan.
A supertaster is a person whose sense of taste is of far greater intensity than the average person, having an elevated taste response.
Non-Mendelian inheritance is any pattern in which traits do not segregate in accordance with Mendel's laws. These laws describe the inheritance of traits linked to single genes on chromosomes in the nucleus. In Mendelian inheritance, each parent contributes one of two possible alleles for a trait. If the genotypes of both parents in a genetic cross are known, Mendel's laws can be used to determine the distribution of phenotypes expected for the population of offspring. There are several situations in which the proportions of phenotypes observed in the progeny do not match the predicted values.
TAS2R16 is a bitter taste receptor and one of the 25 TAS2Rs. TAS2Rs are receptors that belong to the G-protein-coupled receptors (GPCRs) family. These receptors detect various bitter substances found in nature as agonists, and get stimulated. TAS2R16 receptor is mainly expressed within taste buds present on the surface of the tongue and palate epithelium. TAS2R16 is activated by bitter β-glucopyranosides
Cruciferous vegetables are vegetables of the family Brassicaceae with many genera, species, and cultivars being raised for food production such as cauliflower, cabbage, kale, garden cress, bok choy, broccoli, Brussels sprouts, mustard plant and similar green leaf vegetables. The family takes its alternative name from the shape of their flowers, whose four petals resemble a cross.
The Kell antigen system is a human blood group system, that is, a group of antigens on the human red blood cell surface which are important determinants of blood type and are targets for autoimmune or alloimmune diseases which destroy red blood cells. The Kell antigens are K, k, Kpa, Kpb, Jsa and Jsb. The Kell antigens are peptides found within the Kell protein, a 93-kilodalton transmembrane zinc-dependent endopeptidase which is responsible for cleaving endothelin-3.
A phene is an individual genetically determined characteristic or trait which can be possessed by an organism, such as eye colour, height, behavior, tooth shape or any other observable characteristic.
Taste receptor 2 member 38 is a protein that in humans is encoded by the TAS2R38 gene. TAS2R38 is a bitter taste receptor; varying genotypes of TAS2R38 influence the ability to taste both 6-n-propylthiouracil (PROP) and phenylthiocarbamide (PTC). Though it has often been proposed that varying taste receptor genotypes could influence tasting ability, TAS2R38 is one of the few taste receptors shown to have this function.
Laurence Hasbrouck Snyder was a pioneer in human genetics and president of the University of Hawaii.
The evolution of bitter taste receptors has been one of the most dynamic evolutionary adaptations to arise in multiple species. This phenomenon has been widely studied in the field of evolutionary biology because of its role in the identification of toxins often found on the leaves of inedible plants. A palate more sensitive to these bitter tastes would, theoretically, have an advantage over members of the population less sensitive to these poisonous substances because they would be much less likely to ingest toxic plants. Bitter-taste genes have been found in a variety of species, and the same genes have been well characterized in several common laboratory animals such as primates and mice, as well as in humans. The primary gene responsible for encoding this ability in humans is the TAS2R gene family which contains 25 functional loci as well as 11 pseudogenes. The development of this gene has been well characterized, with proof that the ability evolved before the human migration out of Africa. The gene continues to evolve in the present day.
A taster is a person, by means of a human genotype, who is able to taste phenylthiocarbamide (PTC) and its derivative 6-n-propylthiouracil (PROP). PTC tastes bitter to many people (tasters) but is tasteless to others (non-tasters).
Mendelian traits behave according to the model of monogenic or simple gene inheritance in which one gene corresponds to one trait. Discrete traits with simple Mendelian inheritance patterns are relatively rare in nature, and many of the clearest examples in humans cause disorders. Discrete traits found in humans are common examples for teaching genetics.
PTC tasting is a classic genetic marker in human population genetics investigations.
Sarah Anne Tishkoff is an American geneticist and the David and Lyn Silfen Professor in the Department of Genetics and Biology at the University of Pennsylvania. She also serves as a director for the American Society of Human Genetics and is an associate editor at PLOS Genetics, G3, and Genome Research. She is also a member of the scientific advisory board at the David and Lucile Packard Foundation.