Nicotiana benthamiana

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Nicotiana benthamiana
Nicotiana benthamiana plant.jpg
Scientific classification OOjs UI icon edit-ltr.svg
Kingdom: Plantae
Clade: Tracheophytes
Clade: Angiosperms
Clade: Eudicots
Clade: Asterids
Order: Solanales
Family: Solanaceae
Genus: Nicotiana
Species:
N. benthamiana
Binomial name
Nicotiana benthamiana

Nicotiana benthamiana, colloquially known as benth or benthi, is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. [1]

Contents

A synonym for this species is Nicotiana suaveolens var. cordifolia, a description given by George Bentham in Flora Australiensis in 1868. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica (1929), honoring the original author in the specific epithet. [2]

History

The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids, before the introduction of commercial tobacco ( N. tabacum and N. rustica ). Indigenous names for it include tjuntiwari and muntju. It was first collected on the north coast of Australia by Benjamin Bynoe on a voyage of HMS Beagle in 1837. [3]

Description

Nicotiana benthamiana, leaf Nicotiana benthamiana leaf.jpg
Nicotiana benthamiana, leaf

The herbaceous plant is found amongst rocks on hills and cliffs throughout the northern regions of Australia. Variable in height and habit, the species may be erect and up to 1.5 metres (59.1 in) or sprawling out no taller than 200 millimetres (7.9 in). The flowers are white. [4]

Research uses

N. benthamiana has been used as a model organism in plant research. For example, the leaves are rather frail and can be injured in experiments to study ethylene synthesis. Ethylene is a plant hormone which is secreted, among other situations, after injuries. Using gas chromatography, the quantity of ethylene emitted can be measured. [5] Due to the large number of plant pathogens able to infect it, N. benthamiana is widely used in the field of plant virology. [6] It is also an excellent target plant for agroinfiltration. [7]

N. benthamiana has a number of wild strains across Australia, and the laboratory strain is an extremophile originating from a population that has retained a loss-of-function mutation in Rdr1 (RNA-dependent RNA polymerase 1), rendering it hypersusceptible to viruses. [8]

Biotechnology

N. benthamiana is also a common plant used for "pharming" of monoclonal antibodies and other recombinant proteins; for example, the drug ZMapp was produced using these plants. [3]

GMO

Cocaine

In 2022, a genetically engineered N. benthamiana was developed that was able to produce 25% of the amount of cocaine found in a coca plant. [9]

COVID-19 vaccine development

The Quebec City-based biotechnology company, Medicago Inc., uses N. benthamiana as a "factory" to produce virus-like particles over short incubation periods (days) and in high volume, enabling rapid manufacturing capability for a potential COVID-19 vaccine. [10] [11]

In February 2022, Health Canada authorised use of the COVID-19 vaccine called CoVLP (brand name Covifenz) developed from N. benthamiana for preventing infection in adults 18 to 64 years old. [12]

Related Research Articles

<i>Nicotiana</i> Genus of flowering plants in the nightshade family Solanaceae

Nicotiana is a genus of herbaceous plants and shrubs in the family Solanaceae, that is indigenous to the Americas, Australia, Southwestern Africa and the South Pacific. Various Nicotiana species, commonly referred to as tobacco plants, are cultivated as ornamental garden plants. N. tabacum is grown worldwide for the cultivation of tobacco leaves used for manufacturing and producing tobacco products, including cigars, cigarillos, cigarettes, chewing tobacco, dipping tobacco, snuff, and snus.

<span class="mw-page-title-main">Texas root rot</span> Pathogenic fungus

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<i>Phytoplasma</i> Genus of bacteria

Phytoplasmas are obligate intracellular parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission. Phytoplasmas were discovered in 1967 by Japanese scientists who termed them mycoplasma-like organisms. Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation in an artificial medium thus remains a major challenge. Phytoplasmas are characterized by the lack of a cell wall, a pleiomorphic or filamentous shape, a diameter normally less than 1 μm, and a very small genome.

Pharming, a portmanteau of farming and pharmaceutical, refers to the use of genetic engineering to insert genes that code for useful pharmaceuticals into host animals or plants that would otherwise not express those genes, thus creating a genetically modified organism (GMO). Pharming is also known as molecular farming, molecular pharming, or biopharming.

Pantoea stewartii is a species of plant pathogenic bacteria that causes Stewart's wilt of corn, as well as jackfruit-bronzing disease, bacterial leaf wilt of sugarcane, and leaf blight in rice. P. stewartii is a gram-negative bacterium in the Enterobacterales, a group that also includes Escherichia coli and several other human, animal, and plant pathogens. Most research on this bacterial pathogen to date has been done on strains infecting corn as the other diseases have been identified much more recently. Due to being relatively easy to work with in laboratory research, P. stewartii has been used to study a range of processes in bacterial physiology including quorum sensing, bacterial pigment production, endoglucanase enzymes, and siderophore-mediated iron acquisition.

<i>Pseudomonas syringae</i> Species of bacterium

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<i>Odontoglossum ringspot virus</i> Species of virus

Odontoglossum ringspot virus (ORSV) is a plant pathogenic virus that belongs to the family Virgaviridae. It is one of the most common viruses affecting cultivated orchids, perhaps second only to the Cymbidium mosaic virus. It causes spots on leaves and colored streaks on flowers. If a plant is also infected with the Cymbidium mosaic virus, it can lead to a condition called blossom brown necrotic streak.

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<span class="mw-page-title-main">ENOD40</span>

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<span class="mw-page-title-main">Medicago Inc.</span> Canadian biotechnology company

Medicago Inc. was a Canadian biotechnology company focused on the discovery, development, and commercialization of virus-like particles using plants as bioreactors to produce proteins, candidate vaccines, and medications. By using live plant leaves as hosts in the discovery and manufacturing process, the Medicago "Proficia" technology intended to create a rapid, high-yield system for its product candidates. Privately owned by a subsidiary of Mitsubishi Tanabe Pharma, Medicago and its product development programs were terminated by Mitsubishi in February 2023.

<span class="mw-page-title-main">CoVLP</span> COVID-19 vaccine candidate produced in a plant

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<span class="mw-page-title-main">Michael Goodin</span> Jamaican plant virologist (1967–2020)

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Cercosporin is a red toxin created by the fungal genus Cercospora.Cercospora act as pathogens on a variety of plants including corn, tobacco, soybean, and coffee. Cercosporin is a perylenequinone natural product that is photoactivated and uses reactive oxygen species (ROS) to damage cell components.

John M. McDowell is the J.B. Stroobants Professor of Biotechnology at Virginia Polytechnic Institute and State University. His major area of research is phytopathology and plant-pathogen interactions. He has used gene-sequencing technology to examine the genome of Phytophthora capsici and to develop strains of soybean plants that are better able to defend against pathogens.

References

  1. Derevnina, Lida; Kamoun, Sophien; Wu, Chih-hang (20 December 2018). "Dude, where is my mutant? Nicotiana benthamiana meets forward genetics". New Phytologist. 221 (2): 607–610. doi: 10.1111/nph.15521 . PMID   30569612.
  2. "Nicotiana benthamiana". Australian Plant Name Index (APNI), IBIS database. Centre for Plant Biodiversity Research, Australian Government. Retrieved 10 March 2008.
  3. 1 2 Patton J (9 August 2014). "How Owensboro tobacco grew a possible miracle drug to treat Ebola". Lexington Herald-Leader. Retrieved 5 March 2019.
  4. "Nicotiana benthamiana". FloraBase . Western Australian Government Department of Biodiversity, Conservation and Attractions.
  5. Matsukawa, Mizuki; Shibata, Yusuke; Ohtsu, Mina; Mizutani, Aki; Mori, Hitoshi; Wang, Ping; Ojika, Makoto; Kawakita, Kazuhito; Takemoto, Daigo (August 2013). "Nicotiana benthamiana Calreticulin 3a Is Required for the Ethylene-Mediated Production of Phytoalexins and Disease Resistance Against Oomycete Pathogen Phytophthora infestans". Molecular Plant-Microbe Interactions. 26 (8): 880–892. doi: 10.1094/MPMI-12-12-0301-R . PMID   23617417.
  6. Goodin, Michael M; Zaitlin, David; Naidu, Rayapati A; Lommel, Steven A (August 2008). "Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions". Molecular Plant-Microbe Interactions. 21 (8): 1015–1026. doi: 10.1094/MPMI-21-8-1015 . PMID   18616398.
  7. Van der Hoorn, Renier A. L.; Laurent, Franck; Roth, Ronelle; De Wit, Pierre J. G. M. (April 2000). "Agroinfiltration Is a Versatile Tool That Facilitates Comparative Analyses of Avr9/Cf-9-Induced and Avr4/Cf-4-Induced Necrosis". Molecular Plant-Microbe Interactions. 13 (4): 439–446. doi: 10.1094/MPMI.2000.13.4.439 . PMID   10755307.
  8. Bally, Julia; Nakasugi, Kenlee; Jia, Fangzhi; Jung, Hyungtaek; Ho, Simon Y.W.; Wong, Mei; Paul, Chloe M.; Naim, Fatima; Wood, Craig C.; Crowhurst, Ross N.; Hellens, Roger P.; Dale, James L.; Waterhouse, Peter M. (2 November 2015). "The extremophile Nicotiana benthamiana has traded viral defence for early vigour". Nature Plants. 1 (11): 15165. doi:10.1038/nplants.2015.165. PMID   27251536.
  9. "Genetically modified tobacco plant produces cocaine in its leaves". New Scientist. Retrieved 27 November 2022.
  10. "Technologies: Production platform". Medicago Inc. 2020. Archived from the original on 26 January 2021. Retrieved 14 July 2020.
  11. Vishwadha Chander (14 July 2020). "Canada's Medicago begins human trials of plant-based COVID-19 vaccine". National Post. Reuters. Retrieved 14 July 2020.
  12. "Health Canada authorizes Medicago COVID-19 vaccine for adults 18 to 64 years of age". Health Canada, Government of Canada. 24 February 2022. Retrieved 24 February 2022.