Defending champion Serena Williams successfully defended her title, defeating her sister Venus Williams in a rematch of the previous year's final, 4–6, 6–4, 6–2 to win the ladies' singles tennis title at the 2003 Wimbledon Championships. It was her second Wimbledon singles title and her sixth major singles title overall.
Kimberly Po-Messerli and Nicole Pratt were the defending champions. They were both present but did not compete together.
Po-Messerli partnered with Corina Morariu, but lost in the quarterfinals to Virginia Ruano Pascual and Paola Suárez.
Pratt partnered with Alicia Molik, but withdrew before their second round match against Elena Dementieva and Janette Husárová.
Nicole Arendt and Ai Sugiyama were the defending champions but they competed with different partners that year, Arendt with Liezel Huber and Sugiyama with Elena Tatarkova.
Daniela Hantuchová was the defending champion but lost in the fourth round to Amanda Coetzer.
Monica Seles was the reigning champion but did not compete that year.
Lisa Raymond and Rennae Stubbs were the defending champions, but lost in the quarterfinals to Anna Kournikova and Chanda Rubin.
Lisa Raymond and Rennae Stubbs were the defending champions, but lost in the semifinals to Cara Black and Elena Likhovtseva. Elena Dementieva and Janette Husárová won the titles, defeating Black and Likhovtseva in the final, 4–6, 6–4, 6–3.
Serena Williams and Venus Williams were the defending champions, but Serena withdrew from the tournament and Venus chose not to participate with another player.
Martina Hingis and Anna Kournikova defeated Daniela Hantuchová and Arantxa Sánchez Vicario 6–2, 6–7(4–7), 6–1 in the final to win the title.
Martina Hingis and Anna Kournikova were the defending champions, but Hingis didn't participate in the tournament.
Kournikova partnered up with Chanda Rubin, but they lost in the third round to Conchita Martínez and Nadia Petrova.
Serena Williams and Venus Williams won in the final 4–6, 6–4, 6–3, against Virginia Ruano Pascual and Paola Suárez
Venus Williams was the defending champion but lost to her sister, Serena Williams, 2–6, 2–6 in the semifinals. Serena Williams won the title by beating first seeded Jennifer Capriati, 7–5, 7–6(7–4) in the final.
Conchita Martínez was the defending champion but lost in the quarterfinals to Jennifer Capriati.
Serena and Venus Williams were the defending champions but withdrew in the third round.
Martina Hingis and Mary Pierce were the defending champions, but neither participated in this tournament.
Amélie Mauresmo was the defending champion, but lost in Quarterfinals to Serena Williams.
Lisa Raymond and Rennae Stubbs were the defending champions, but lost in third round to Kim Clijsters and Meghann Shaughnessy.
Virginia Ruano Pascual and Paola Suárez were the defending champions and successfully defended their title, defeating Lisa Raymond and Rennae Stubbs 6–4, 6–2 in the final.
Jennifer Capriati was the defending champion, but lost in semifinals to Patty Schnyder
Arantxa Sánchez Vicario and Nathalie Tauziat were the defending champions, but Tauziat did not compete in this edition. Sánchez Vicario teamed up with Daniela Hantuchová and lost in first round to Elena Dementieva and Janette Husárová.
Elena Dementieva and Janette Husárová were the defending champions, but had different outcomes. Dementieva had to withdraw due to a left foot strain, while Husárová teamed up with Elena Likhovtseva and reached the semifinals.
Amélie Mauresmo was the defending champion, but lost in second round to Anne Kremer.
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Elena Dementieva 
Janette Husárová 
Arantxa Sánchez Vicario 
