| 2010 Sparta Prague Open | |
|---|---|
| Date | 10–16 May |
| Edition | 1st |
| Category | ITF Women's Circuit |
| Surface | Clay |
| Location | Prague Czech Republic |
| Venue | TK Sparta Prague |
| Champions | |
| Singles | |
 Lucie Hradecká | |
| Doubles | |
 Ksenia Lykina /  Maša Zec Peškirič | |
The 2010 Sparta Prague Open was a professional tennis tournament played on clay courts. It was part of the 2010 ITF Women's Circuit. It took place at TK Sparta Prague in Prague, Czech Republic between 10 and 16 May 2010 with a $50,000 prize money.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| CZE | Lucie Hradecká | 77 | 1 |
| CZE | Sandra Záhlavová | 86 | 2 |
 AUS | Jelena Dokić | 106 | 3 |
| SLO | Maša Zec Peškirič | 112 | 4 |
| RUS | Ksenia Pervak | 118 | 5 |
 BLR | Darya Kustova | 135 | 6 |
 ITA | Anna Floris | 151 | 7 |
| CZE | Andrea Hlaváčková | 154 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry by a lucky loser spot:
Lucie Hradecká defeated 
Ajla Tomljanović, 6–1, 7–6(7–4)
Ksenia Lykina / Maša Zec Peškirič defeated Petra Cetkovská / Eva Hrdinová, 6–3, 6–4
