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Martina Hingis and Flavia Pennetta were the defending champions, but chose not to participate this year.
Andrea Hlaváčková and Lucie Hradecká were the defending champions, but chose to participate at the 2015 WTA Finals instead.
Bethanie Mattek-Sands and Lucie Šafářová were the defending champions, but Šafářová withdrew before the tournament due to a bacterial infection. Mattek-Sands played alongside Sabine Lisicki, but lost in the second round to Anastasia and Arina Rodionova.
Tímea Babos and Kristina Mladenovic were the defending champions, but chose not to participate together. Babos played alongside Yaroslava Shvedova, but lost in the quarterfinals to Irina-Camelia Begu and Monica Niculescu. Mladenovic teamed up with Caroline Garcia, but lost in the quarterfinals to Andrea Hlaváčková and Lucie Hradecká.
Martina Hingis and Sania Mirza won the title, defeating Ekaterina Makarova and Elena Vesnina in the final, 6–1, 6–7(5–7), [10–3].
Belinda Bencic and Kristina Mladenovic were the defending champions, but chose not to participate this year.
Bethanie Mattek-Sands and Lucie Šafářová were the defending champions, but decided not to compete together. Mattek-Sands played alongside Heather Watson, but lost in the quarterfinals to Andreja Klepač and Katarina Srebotnik. Šafářová teamed up with Tímea Babos, but lost in the first round to Kateryna Bondarenko and Olga Savchuk.
Han Xinyun and Christina McHale were the defending champions, but chose not to participate this year.
Bethanie Mattek-Sands and Lucie Šafářová were the defending champions, but Mattek-Sands was unable to compete due to injury. Šafářová played alongside Barbora Strýcová, but lost in the semifinals to Lucie Hradecká and Kateřina Siniaková.
Sania Mirza and Monica Niculescu were the defending champions, but lost in the first round to Nicole Melichar and Anna Smith.
Ekaterina Alexandrova was the defending champion, but lost in the quarterfinals to Antonia Lottner.
Abigail Spears and Katarina Srebotnik were the defending champions, but chose not to compete together. Spears played alongside Alicja Rosolska, but lost in the first round to Mihaela Buzărnescu and Alizé Cornet. Srebotnik teamed up with Shuko Aoyama, but lost in the first round to Anastasia Pavlyuchenkova and Olga Savchuk.
Latisha Chan and Martina Hingis were the defending champions, but Hingis retired from professional tennis at the end of 2017. Chan played alongside her sister Chan Hao-ching, but lost in the second round to Hsieh Su-wei and Barbora Strýcová.
Gabriela Dabrowski and Xu Yifan were the defending champions, but chose not to participate this year.
Alison Van Uytvanck was the defending champion, but chose not to participate.
Garbiñe Muguruza was the defending champion and successfully defended her title, after Victoria Azarenka retired in the final with a leg injury with the score at 6–1, 3–1.
The 2020 TK Sparta Prague Open was a women's tennis tournament played on Clay in Prague, Czech Republic, and was part of the 2020 WTA 125K series.
Kim Clijsters was the defending champion, having won the previous edition in 2002, but chose not to participate.
Fiona Ferro was the defending champion, but she chose not to participate, as she was competing at the Summer Olympics.
This was the first edition of the tournament.
The women's doubles of the 2021 I.ČLTK Prague Open tournament was played on clay in Prague, Czech Republic.